LOCUS       AP022235                4820 bp    DNA     circular BCT 22-JUL-2020
DEFINITION  Enterobacter cloacae plasmid pWP8-W18-CRE-04_7 DNA, complete genome,
            strain: WP8-W18-CRE-04.
ACCESSION   AP022235
VERSION     AP022235.1
DBLINK      BioProject: PRJDB6962
            BioSample: SAMD00194608
            Sequence Read Archive: DRR199444
KEYWORDS    .
SOURCE      Enterobacter cloacae
  ORGANISM  Enterobacter cloacae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1
  AUTHORS   Sekizuka,T., Itokawa,K., Yatsu,K., Inamine,Y. and Kuroda,M.
  TITLE     complete genome sequences of Enterobacter cloacae str.
            WP8-W18-CRE-04 isolated from wastewater treatment plant effluent
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4820)
  AUTHORS   Sekizuka,T., Itokawa,K., Yatsu,K., Inamine,Y. and Kuroda,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-DEC-2019) Contact:Tsuyoshi Sekizuka National Institute
            of Infectious Diseases, Pathogen Genomics Center; 1-23-1 Toyama,
            Shinjuku-ku, Tokyo 162-8640, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: flye v2.5; minimap v0.2-r124; racon v1.1.0;
            circlator v1.5.3; Pilon v1.18; SPAdes v3.11.1; A5-miseq v20140604
            Genome Coverage       :: 400x
            Sequencing Technology :: Sequel; NextSeq 500
            ##Genome-Assembly-Data-END##
            Annotated by DFAST v.1.2.3 (https://dfast.nig.ac.jp).
FEATURES             Location/Qualifiers
     source          1..4820
                     /organism="Enterobacter cloacae"
                     /mol_type="genomic DNA"
                     /strain="WP8-W18-CRE-04"
                     /isolation_source="wastewater treatment plant effluent"
                     /db_xref="taxon:550"
                     /plasmid="pWP8-W18-CRE-04_7"
                     /geo_loc_name="Japan:Tokyo"
                     /lat_lon="35.6316 N 139.7460 E"
                     /collection_date="2018-02-13"
     gene            complement(101..301)
                     /locus_tag="WP8W18C04_P70010"
     CDS             complement(101..301)
                     /locus_tag="WP8W18C04_P70010"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BBT47459.1"
                     /translation="MIIKPYRRGSDNVQPIIRPDANPASQPITGVLLGKKSGGGGGCSV
                     STDAGQKYISHKKTPHSGNSY"
                     /name="hypothetical protein"
     gene            complement(694..1095)
                     /locus_tag="WP8W18C04_P70020"
     CDS             complement(694..1095)
                     /locus_tag="WP8W18C04_P70020"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BBT47460.1"
                     /translation="MALSEAKKQQQRDARALLEGFWPEAFNFSQPRPLKLGVFEELVAD
                     AKLRGLPFDAAILKAAMKSYTSRYVYQQAVSKIAERTGLDGSPCGEVSAEQKAYAKVQL
                     QRIDAKAKARKKALEADAVAKPVPNEPDA"
                     /name="hypothetical protein"
     gene            complement(1250..1459)
                     /locus_tag="WP8W18C04_P70030"
     CDS             complement(1250..1459)
                     /locus_tag="WP8W18C04_P70030"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BBT47461.1"
                     /translation="MTELEKQLLSALSQLQQDYSTRLDEWEHAFAEWRTMSGLMQRENA
                     ALNARVSDLSRQIRTLSEQLDRLS"
                     /name="hypothetical protein"
     gene            complement(1463..1948)
                     /locus_tag="WP8W18C04_P70040"
     CDS             complement(1463..1948)
                     /locus_tag="WP8W18C04_P70040"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BBT47462.1"
                     /translation="MSSLFTLAKDLEQKSKAQQQSTGEMLKAAFSEHEKSVRTELNASA
                     KRISAAISAHEQGVNEAMQSNQRSTLRLVGRTWVTVVTVSVLLIATSGSVLWWQGQQMV
                     ANYTTIEAQKATQAMLSARNNGVQLSTCGDARRTCVKVNPGPGAFGDDSTWMILAGK"
                     /name="hypothetical protein"
     gene            complement(2626..2970)
                     /locus_tag="WP8W18C04_P70050"
     CDS             complement(2626..2970)
                     /locus_tag="WP8W18C04_P70050"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BBT47463.1"
                     /translation="MSEKRNKMLTMWVTEDEHRRLLERCDGKQLAAWIRQTCLDEKPAR
                     AGKLPSIAPALLRQLAGMGNNLNQIARQVNAGGGTGHDRVQVVAALMAIDAGLEQLREA
                     VLAKGPDDDR"
                     /name="hypothetical protein"
     gene            complement(3110..3745)
                     /locus_tag="WP8W18C04_P70060"
     CDS             complement(3110..3745)
                     /locus_tag="WP8W18C04_P70060"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BBT47464.1"
                     /translation="MITRLIFIAAMAALTAPTYAAAIGNKDCSHPLSASGLHALESSPD
                     YAAVKTEFTKALIAACKEGKSRDDIGFPAADELAGKAADHYVSTHDPKTTDPKAIRNQA
                     GAIYNAWLGGYTGFDLPEDSKKKPARQPMNTAACEQLTKDAIQASIPPNVTITLEQYKK
                     SAGQLGMICMIALQQGYDGKPRNNELLGSLSPEVTEMFNRAYDAGAAQ"
                     /name="hypothetical protein"
     Typing          4035..4416
                     /name="rep_cluster_2373"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="80.362"
                     /coverage="87.0"
                     /evalue="1.5500000000000002e-72"
     Typing          complement(1642..2636)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="84.94"
                     /coverage="86.0"
                     /evalue="1.84e-178"
ORIGIN
        1 caacgggcag tccaaggtgt ttaaacccgt cgtgccgacg cgggatagcg gggagagaat
       61 cgtatgtaaa ccctaactca aagtgtacgt aaatcccaac ttaatacgag ttaccagaat
      121 gcggcgtttt cttgtgagat atatatttct gtccggcatc cgtcgataca gaacagcccc
      181 cgccgccgcc tgatttcttt ccgagtaata cgccggttat aggctggctt gctgggttag
      241 cgtccgggcg gataattggc tgaacgttat cactcccacg gcgataaggt ttaataatca
      301 ttcggctaac tccgcttaaa attaaaatgg ttttctgtac gcctatcacg gcgaagataa
      361 tattcttcat tcgttttaat cgtcgcgact aacatattaa acaccgctaa ccacacgaaa
      421 aacaggctct gggctgcgag cgccctataa gatctttata agattaataa aaagatctta
      481 taagtgaaat ctttacgact ggggataaaa atcgattttc gattcggcgg ttttcaaagt
      541 ttaggtttag aagactaatt taccataaca tccgttcagc gcaccgtcta aacggcacac
      601 agggcgattt tacgccctga cgcccataaa tccctgcctt accccccgaa aaccacccag
      661 aacgccgcag gttaattttg aggccgtgca tagctatgca tccggctcgt tgggcaccgg
      721 tttcgccacg gcatcggcct ctaatgcttt ttttcgtgcc tttgctttcg catcaatgcg
      781 ctggagctga acctttgcat aggctttctg ctccgctgac acctcgccgc acggcgaacc
      841 atcaagcccc gtgcgctccg ctatcttgct caccgcttgc tggtagacgt agcggctggt
      901 gtacgacttc atggcggcct tcaggatcgc cgcatcgaaa ggcagtcccc gcaacttcgc
      961 atcggcgacc aactcctcaa aaacccccag tttcaacggg cgcggctggc tgaagttaaa
     1021 cgcctcgggc cagaacccct cgagcagggc ccgggcgtcg cgttgctgct gttttttggc
     1081 ttcggacagc gccataactt ctcctcataa actcactgat gcggccagcc gccctacatt
     1141 gacgggccgt gatacgttcg cggcgggcgc ggcgctggcg cgtcttcaag ttcgcgcgcc
     1201 agcgcctgcc ttctcagcgc cgcgcgctgc tgctctaccg acgcaatttc taggacaagc
     1261 ggtctaactg ctcgcttaaa gtccgtatct gcctgctcaa gtcggagacg cgcgcgttca
     1321 gcgccgcgtt ctcccgttgc ataagaccag acatcgtccg ccattccgcg aaggcgtgct
     1381 cccactcgtc cagccttgtc gaatagtcct gctgtagctg cgataacgcg ctcagcaact
     1441 gcttttccag ctccgtcatt ccctatttcc ccgccagtat catccaggtc gaatcgtccc
     1501 cgaacgcgcc cgggcccggg ttcaccttga cgcacgtccg cctcgcgtcc ccgcaggtcg
     1561 acagctgcac gccgttgttc ctcgccgaca gcatcgcctg cgtggccttc tgcgcctcga
     1621 tggtcgtgta attcgcgacc atctgctgcc cctgccacca cagcacgctc ccgcttgttg
     1681 cgataagcag cactgacacc gtcaccactg tgacccacgt ccgcccgacc agccgcagcg
     1741 tgctgcgctg gttcgactgc atcgcctcgt tcaccccctg ctcgtgggcg ctgatggcgg
     1801 cgctgattcg cttcgcgctt gcgttcagtt ccgttcttac agacttctcg tgctcgctga
     1861 aggcggcttt cagcatctcg ccggtactct gctgctgcgc tttcgatttc tgctctaagt
     1921 ccttcgccag cgtgaaaaga ctgctcatag agggcccctt ttagtcgaat gttttgcccc
     1981 ccgtccgggt cggcaatgct gatactgctt ttcgtggtgc gtaccacctc aaaccctgac
     2041 cgcgtcagcg cctcggtgac gtcctgccgc gttgcaagct caccggagga ggccagcgcg
     2101 agcagcccgc gcgtaatggc ctgagcggct tcctgcttcg ttttgggcag ggccgacggc
     2161 gtgaccagcg cccggcggtt ctccggcgcg ttcgggtcgt gaagccccag cctgccgttg
     2221 accagcgttt gccaggcatc gatgcgcggg cggtcggccc ggtcgtaata gggctggagc
     2281 cgccttcccg tcaacaggtc ggtattcggg ataaggaaat tcagctccag ccgccccttg
     2341 tcggcgtgct ccacccacag cacgctgtac tggtcttttt cgaggccggg catcagaacc
     2401 cgctcgaagc ttgccatcaa tttttcgcgc tgaccgggcg gcaggtcggc ctccgcaaac
     2461 gacagcacgc cggaggtgta cttcttggcg tagggcgacg cgtcgataag ttcacgcacc
     2521 gactctgcgc ggccctgcag aacgctggcg ccctcgcgct gccggtcttt ccccagcagg
     2581 tagtccaccg gcccggcgcc gcccccgcgc ccccgggggt gaaatttaac gatcatcgtc
     2641 cggccccttt gccagcaccg cctcgcggag ctgctccagt ccggcatcga tggccatcag
     2701 tgccgccacg acctgcaccc ggtcgtgccc ggtaccgccc ccggcgttaa cctgccgggc
     2761 gatctggttc aggttattgc ccatgcccgc cagttggcgc agtagcgccg gcgcgatcga
     2821 aggaagtttg ccggcacgcg ccggcttttc atcaaggcac gtttgccgaa tccatgccgc
     2881 gagctgttta ccgtcgcaac gctccagcag tcgacggtgc tcgtcctcag tcacccacat
     2941 cgtgagcatt ttgttgcgtt tctctgacaa gcgggcctcc tgagaacacc gggacagacg
     3001 gggaactttt tccggtctgc acatggttag gcaagattaa atgatgcggt tatcaaacct
     3061 tgttaatacg atacccatgc atattgagtc gggattgccg ccaaaaaacc tactgtgccg
     3121 ctcctgcatc atatgcacgg ttaaacattt cagtgacttc aggacttaac gagccaagta
     3181 attcattgtt tcgtggcttg ccgtcgtaac cctgctgcaa ggcgatcatg caaatcatac
     3241 cgagttggcc agcactcttt ttgtactgtt ccaacgtgat agtgacgttt ggcggaatgc
     3301 tggcctgtat cgcatctttc gtcagttgtt cgcatgcagc agtattcatg ggctgacgcg
     3361 cgggtttctt ttttgaatct tcaggtaggt caaaacctgt atagccccct aaccaggcgt
     3421 tataaatagc cccggcctga ttacggatag ctttagggtc tgtggtttta gggtcatgcg
     3481 tcgaaacgta gtgatcagca gccttccctg ccaattcgtc agcggcagga aagcctatgt
     3541 catcacggct ttttccctct ttacaggctg caatgagagc cttggtaaac tcggttttca
     3601 cagcagcata gtcaggtgaa ctttcaagcg catggagacc agaggcgctg aggggatggg
     3661 agcaatcttt attccctata gcggcagcat aagtaggtgc cgtgagagcc gccatggcag
     3721 caataaatat caatcgtgta atcatttttt cctttgggca ggtttcgggg cgcagccctg
     3781 aaccagtcac gtagcgctag cggagtgtat actggcttaa tatgctaaat cggagtggtt
     3841 gaaaggtcaa aaggatcatg ggattaaggc gtaatcagac cggcagagga gtggcacggg
     3901 taaatggcag tggagatgat atgacgcctc ctcgctcatt cgctcactgc gttcggtcat
     3961 gaggctgcgg cgggcgttgc gggtcatcgt gaggaagtgt aggacaggtg cggcaggcaa
     4021 gaagggtcag agcaaagggc gcggggaacc cgttttacca taggctccgc ccccctgacg
     4081 agactcacag aaatcggatg atttccggcg ctcaaatcag tggtggcgaa acccgacaca
     4141 gcttaaagat gccagacgtt tccctctggc ggctccctcg tgcgctcctg tgttcccgtc
     4201 ttatgggttg tcggtgtctt ccgcaattat ggctgcggtt tctcatacca cgcctgacac
     4261 tcaactccac gtagacggtt cgctccaagc tggactgtat gcacgaaccc cccgttcagt
     4321 ccgactacca cgccaagtaa ggtaactatc aacttgagtc caacccttac agacacgacg
     4381 aatcgccatt ggcggtagcc actggtaact gatttagagg agtgagtctt gaagttatgg
     4441 gccgtttaag gctaaactga aaggacagat ttggtgactg cgctcctcca agccagttac
     4501 cttggttcaa agagttgctg ctctgcgaac cttcgaaaaa accgccttgc tgggcggttt
     4561 tttcgtttca gagattacat ctcgtcacac gtaactgtca acgcaacgat ttaacccagg
     4621 cccacacaat caaacccttt gcagagcaag acaaacaata aacgacctag acgacgggcg
     4681 ctacgccctc ttggttttta tatacccact agagcaggca caacaggcac agtacagcca
     4741 gccggtaacg ctacgtggat tctgtagctg tggcagggct acgacattgc agaactcaca
     4801 ggataaaaga cctaccacgg
//
