LOCUS       AP022234                5010 bp    DNA     circular BCT 22-JUL-2020
DEFINITION  Enterobacter cloacae plasmid pWP8-W18-CRE-04_6 DNA, complete genome,
            strain: WP8-W18-CRE-04.
ACCESSION   AP022234
VERSION     AP022234.1
DBLINK      BioProject: PRJDB6962
            BioSample: SAMD00194608
            Sequence Read Archive: DRR199444
KEYWORDS    .
SOURCE      Enterobacter cloacae
  ORGANISM  Enterobacter cloacae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1
  AUTHORS   Sekizuka,T., Itokawa,K., Yatsu,K., Inamine,Y. and Kuroda,M.
  TITLE     complete genome sequences of Enterobacter cloacae str.
            WP8-W18-CRE-04 isolated from wastewater treatment plant effluent
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5010)
  AUTHORS   Sekizuka,T., Itokawa,K., Yatsu,K., Inamine,Y. and Kuroda,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-DEC-2019) Contact:Tsuyoshi Sekizuka National Institute
            of Infectious Diseases, Pathogen Genomics Center; 1-23-1 Toyama,
            Shinjuku-ku, Tokyo 162-8640, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: flye v2.5; minimap v0.2-r124; racon v1.1.0;
            circlator v1.5.3; Pilon v1.18; SPAdes v3.11.1; A5-miseq v20140604
            Genome Coverage       :: 400x
            Sequencing Technology :: Sequel; NextSeq 500
            ##Genome-Assembly-Data-END##
            Annotated by DFAST v.1.2.3 (https://dfast.nig.ac.jp).
FEATURES             Location/Qualifiers
     source          1..5010
                     /organism="Enterobacter cloacae"
                     /mol_type="genomic DNA"
                     /strain="WP8-W18-CRE-04"
                     /isolation_source="wastewater treatment plant effluent"
                     /db_xref="taxon:550"
                     /plasmid="pWP8-W18-CRE-04_6"
                     /geo_loc_name="Japan:Tokyo"
                     /lat_lon="35.6316 N 139.7460 E"
                     /collection_date="2018-02-13"
     gene            complement(101..502)
                     /locus_tag="WP8W18C04_P60010"
     CDS             complement(101..502)
                     /locus_tag="WP8W18C04_P60010"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BBT47453.1"
                     /translation="MTRVSISEAARLTGKSRTTLHRLIKTGELSTCSGERNTKMVDISE
                     LLRVFGPFDQPKNEHLTRQVPEQRDTAPADQSEHVIRQLKQQIEHLNILILSKDSHIDS
                     LKQAMLLLENHQAKLTEPPAPWWKFWKKS"
                     /name="hypothetical protein"
     gene            904..1518
                     /locus_tag="WP8W18C04_P60020"
     CDS             904..1518
                     /locus_tag="WP8W18C04_P60020"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /inference="similar to AA sequence:RefSeq:WP_011183217.1"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BBT47454.1"
                     /translation="MGKHKVFISYHHANDQYYKNALEKMNEEHEIFVNRSVSLGDIDED
                     EAPQKIREIIRDEYLRDTSVLILLVGTETKNRKHVDWELYSSMRDSTINKKSGILVVNL
                     PSTNTTYIRSTHGTNEKSEVHPTVTEWFSIDKRSTYEERFPYMPARIIDNFMKSGSYIS
                     VVNWNQIENNPETLRKLIDMTYNDREKATYDMSREMRMHNS"
                     /name="hypothetical protein"
     gene            complement(1555..2262)
                     /locus_tag="WP8W18C04_P60030"
     CDS             complement(1555..2262)
                     /locus_tag="WP8W18C04_P60030"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BBT47455.1"
                     /translation="MKTFVTLWTIVFLAFIAVMCTVDISNYIPSVYTVFSKNRPLVTGA
                     LSILLISVFIWLMVSLYLLKKYSVKVEKLSLGGVNILFNESATLYKKSVKNHLDSKRAI
                     FKLRKNVDAFDEVISSYYQTYQFIRDEMKILNPKKDREIYDFSNEMLMVLNRFLTNYQN
                     NYKRWYKYISDKDEVVDRVSGDVMRFHATSINKIQMQYYDYERILKGFKDVNRFFVNDV
                     KPKFGINTDKWNW"
                     /name="hypothetical protein"
     gene            complement(2259..3164)
                     /locus_tag="WP8W18C04_P60040"
     CDS             complement(2259..3164)
                     /locus_tag="WP8W18C04_P60040"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BBT47456.1"
                     /translation="MHNAALELFNERLPHKPYFSDDLHFGVRIAGKERAILAKYIQFNQ
                     PHAMFWLGFDVDRAGSSIDWSDRNAPAPTLTITNPENGHAHLLYALENSIRTAPDGKIK
                     PLRYAASVENALRKKLEADAGYSGLICKNPNHNHWKIAVWQPKLYTLDWLADFLDLNTG
                     NDKEIVADYGLGRNCTLFDKTRKWAYRAIRQGWPEYEQWLQACYERACAYNLQFARPLD
                     ENEVRGIAKSIAKWTSNKFSPEEFSKFVDITHSSEIQSKRGKKSGQSRRKGSLEEIKPW
                     VAMGISRRKYFYIKKNGEIR"
                     /name="hypothetical protein"
     gene            complement(3412..3687)
                     /locus_tag="WP8W18C04_P60050"
     CDS             complement(3412..3687)
                     /locus_tag="WP8W18C04_P60050"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BBT47457.1"
                     /translation="MSKWNIKFTRNGADDLNYWRDTDKKKFERIRQLLLSIEADPLTGI
                     GKPERLRHHKDPALYSRRIDQSHRLVYSIQNGEIIIYAARYHYGDK"
                     /name="hypothetical protein"
     gene            complement(3668..3970)
                     /locus_tag="WP8W18C04_P60060"
     CDS             complement(3668..3970)
                     /locus_tag="WP8W18C04_P60060"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BBT47458.1"
                     /translation="MYLFAYMFYAKPQQMGVNNMHAINFTDARKHFAETMKRVTDDAEP
                     VRVMRRDAPDIVMIDAGEYEALIETVYLFSNPANAEHINESMKQAERGEFVEVEY"
                     /name="hypothetical protein"
ORIGIN
        1 gtatttttcg tggtgaggag cgttcagaga tctgacgatt ttcgggaagg ttcaggaact
       61 ccccggaatg gccggagagg aaaactgaag catgacgggg tcaggatttt ttccagaatt
      121 tccaccacgg cgccgggggt tcagttaatt ttgcctggtg attttcaaga aggagcatgg
      181 cctgtttcag gctgtcaatg tgcgaatcct tcgataaaat caatatgtta aggtgttcaa
      241 tctgctgttt cagttgacga atgacatgtt cactctggtc agcaggcgct gtatcacgct
      301 gttcaggtac ttgtcgagta aggtgttcat tcttcggttg gtcaaacggt ccgaaaacac
      361 gtaaaagctc tgatatatcg accatttttg tatttcgttc accgctacaa gtggacagtt
      421 cacctgtttt gattaacctg tgcagcgttg tccggctctt acccgtcagc cgtgcagcct
      481 cactaataga cactctggtc atcagttatc atcctttaat acattcagtc tcaaggaaac
      541 aggggccagc ttacgacata ctggtccaga agtgatgtgt ttttcagaag ggcgatgaaa
      601 cacaggagtg accgtgcaaa atgcaggcaa caaatgactt tccgcttcct cgcgcattcg
      661 ctcactacgt tcggtcataa gactgcggcg agcggtacgt aagaataaag tgaggtgata
      721 gctctcttct caaggcgttt agggaagcaa cgagcccgcc ctaaaccagt catggcgaga
      781 ccttgctgaa gggaccctat gtgtgctggc tgagaaaaaa agagctctct aaaagagagc
      841 tgttatctga tataggttca agtgcaaaat agagtgcaca caaacaaaaa aaggacaata
      901 aaaatgggaa agcataaggt attcattagc taccaccatg cgaatgatca gtactacaaa
      961 aacgcacttg aaaaaatgaa tgaggaacat gaaatattcg tgaatcgctc agtatcatta
     1021 ggtgatatag atgaagatga agccccacaa aaaataagag agataataag agatgaatat
     1081 cttagagata catcagttct tatccttctt gttggaactg aaacaaaaaa cagaaaacac
     1141 gtagattggg aactttactc tagcatgaga gatagtacca ttaacaaaaa atcaggaatt
     1201 ttagtcgtca atctcccgtc aacaaatact acgtacattc gttcaacaca tggaactaat
     1261 gaaaaatcag aagttcatcc taccgtaact gaatggtttt ctattgataa gcgctcaact
     1321 tatgaggaaa ggttcccata tatgccagca agaataatag acaacttcat gaagagcggg
     1381 tcttatatat cagtggtcaa ctggaatcaa atagaaaaca acccagaaac actcagaaaa
     1441 ttaatagaca tgacttataa cgatagagaa aaagcaacgt atgatatgtc gagagaaatg
     1501 agaatgcata attcatagta ctatccaagg agtagccttg gctactcctt taatttacca
     1561 gttccactta tctgtattga ttccaaactt aggcttcaca tcattaacaa agaaccggtt
     1621 aacatcttta aaacctttta aaatacgctc ataatcataa tactgcattt gtattttatt
     1681 tatgctggtt gcatgaaatc tcatgacatc accgctaacc ctatcaacaa cctcatcttt
     1741 gtctgatatg tacttatacc aacgcttgta gttgttctgg tagttagtta gaaatctatt
     1801 taacaccatg agcatctcat tgctgaaatc ataaatctca cgatcttttt tagggtttag
     1861 tattttcatt tcatcacgaa taaattggta agtctgataa tatgatgaaa taacctcatc
     1921 aaaggcatca acgttttttc ttaatttgaa aattgctctt tttgaatcta ggtgattttt
     1981 tacactcttt ttgtaaagtg tagcactctc attgaaaagt atattcactc caccaaggct
     2041 aagtttctca accttaaccg aatacttttt aagtaagtag agggatacca tcagccaaat
     2101 aaaaacagat atcaaaagaa tagaaagggc gccagttaca agaggtctat tcttactaaa
     2161 aactgtgtaa acagacggaa tgtaattaga tatatcaaca gtacacatca cggcaataaa
     2221 tgctaaaaaa actatagtcc aaagagttac gaaggttttc atcttatttc accatttttc
     2281 ttgatataaa aatacttccg cctgcttatt cccatagcta cccaaggttt tatttcctct
     2341 aggcttcctt tcctccttga ctgtcctgac tttttccctc tctttgattg aatttcagag
     2401 gaatgagtaa tatcaacaaa tttactgaat tcttcaggtg aaaatttatt tgatgtccat
     2461 ttagcaatgc tcttagctat ccctcttacc tcattttcat ccagaggccg tgcaaattgc
     2521 aggttgtatg cgcaagcgcg ttcgtagcag gcttgcagcc actgctcata ctcaggccag
     2581 ccctgacgga tggcgcgata tgcccactta cgggttttgt cgaacaacgt gcagttacgg
     2641 cctaagccgt agtcggcaac gatttccttg tcattccctg tgttcagatc gagaaagtcg
     2701 gccagccagt ccagagtgta gagcttcggc tgccacacgg cgattttcca gtggttgtgg
     2761 ttcggatttt tgcagatgag ccccgaatat ccagcgtcag cctctaattt tttacgtagc
     2821 gcattctcca cggaggcggc gtagcgtaac ggttttattt tgccgtccgg cgcggtgcgt
     2881 atcgagtttt ccagcgcgta cagcaggtgc gcgtgtccgt tttcggggtt agttatggtc
     2941 agcgtcggtg caggcgcgtt gcggtcgctc cagtcgatgg aggaaccggc gcggtcaacg
     3001 tcaaatccca gccagaacat ggcgtgaggc tgattgaact ggatatattt cgccagaata
     3061 gcgcgttcct taccggcgat acggacacca aagtgcagat cgtccgaaaa gtacggcttg
     3121 tgtggtaagc gttcgttgaa aagttcgagt gcggcgttat gcaatgtgcc ctgcgcttat
     3181 ggcgggctgt tgtctggcac ggttcatgtg ctaatatcct ttcaaggttt cgacctagcc
     3241 ctggttatca tgcgatccgc caagatatga catgatttcc ggggctttgt tttttctgag
     3301 gctaacgtta caccgacttt gcctttgtgt cgatctgaca gcgacttacc ctaacatccg
     3361 ttgtacgcct tgcatgaaaa gcccgcatca agcgggctat atcgactttt gctatttgtc
     3421 accatagtga taacgggctg cgtagatgat gatttctccg ttctggattg aatagaccag
     3481 acggtggctc tgatcaatcc tgcgggagta gagtgccgga tctttgtggt gccgcagccg
     3541 ttcgggtttt cctatacctg tcagcggatc agcctcgatg ctgagcaaca gttgccggat
     3601 acgctcaaac ttctttttgt cagtatcacg ccagtagtta aggtcatcag cgccattcct
     3661 agtgaactta atattccact tcgacaaatt cgccgcgctc cgcctgtttc attgactcat
     3721 tgatatgctc tgcgttcgcc ggattgctga acagatagac cgtttcgatt agcgcctcat
     3781 actctcccgc atcgatcata acaatatcag gcgcatcacg gcgcatgaca cgtaccggtt
     3841 cagcatcatc agtgacgcgc ttcatcgtct cggcaaaatg tttacgggca tcagtaaaat
     3901 tgattgcatg catgttgtta acccccattt gttggggctt agcatagaac atgtacgcaa
     3961 ataagtacat aaagatattt ccctaaaaac ccacaaccaa atcagctcaa aatgtgaact
     4021 caatcgaata accacaaaaa caacaagtca atacattgaa gcataatttc aagcaattac
     4081 atttatgaag tgcggcacaa tttcgtaccg ctaggagctt agctcctaaa acgagcaaag
     4141 gaaaagtaaa tggcaaaaca ttggtctggg caagaatgtc caaaaacagg tacttacggt
     4201 caatatcatg ataccgacaa ctcatacgct ggttctgaat atgatcgcca tgtgagcaag
     4261 ggtgataagt tccctccgag caaaaacaac caccacttcc aagaaaaata aaatatcttc
     4321 agaagtctaa actaaaagcc ctctggggct ttttttgtac ctgccattca cttgatttac
     4381 cataacatcc gttatcagca ccgcctgaga tgccctcagg gcgtttctga cgggaaaccg
     4441 gtaaacaagg caacaaacca cccgaaaacc cgccagaaac gcgcaggctc tgtttttacg
     4501 gcatgcatga gtatgcgcac aagtgcatag cgcggaaatt gacaccattc agccagggga
     4561 agtcctgttt ccggcgttct tcccatccga aaacggtgaa cggggacgca atttattgcg
     4621 tggccgtgaa gcgttgccgg cgcagccgga aaaccggttt tcagcacgtt caggaatcgg
     4681 ttcgtccacg gccggcaatc acgttatggc aagtcgccgg acgactcacc acacgattcc
     4741 ccggcgactc ttcactagag tcgtacccag agtcgccacg tctaaaaaac aaccaaacgg
     4801 ggagtcggat ctagagtcgg gtcgttatta actcattgtt tttataggcc tgagtcgtta
     4861 ccgactctat gacgactcgc cggggactct atgacgactc gtcatagagt cggatctata
     4921 taaataaaat ttaaaaaacg aaatttcagt aaagtaagct gctgatatta aatacaaatc
     4981 tgcaaaatta aaattttgcg tggggtgtgg
//
