LOCUS       AP022233                5943 bp    DNA     circular BCT 22-JUL-2020
DEFINITION  Enterobacter cloacae plasmid pWP8-W18-CRE-04_5 DNA, complete genome,
            strain: WP8-W18-CRE-04.
ACCESSION   AP022233
VERSION     AP022233.1
DBLINK      BioProject: PRJDB6962
            BioSample: SAMD00194608
            Sequence Read Archive: DRR199444
KEYWORDS    .
SOURCE      Enterobacter cloacae
  ORGANISM  Enterobacter cloacae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1
  AUTHORS   Sekizuka,T., Itokawa,K., Yatsu,K., Inamine,Y. and Kuroda,M.
  TITLE     complete genome sequences of Enterobacter cloacae str.
            WP8-W18-CRE-04 isolated from wastewater treatment plant effluent
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5943)
  AUTHORS   Sekizuka,T., Itokawa,K., Yatsu,K., Inamine,Y. and Kuroda,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-DEC-2019) Contact:Tsuyoshi Sekizuka National Institute
            of Infectious Diseases, Pathogen Genomics Center; 1-23-1 Toyama,
            Shinjuku-ku, Tokyo 162-8640, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: flye v2.5; minimap v0.2-r124; racon v1.1.0;
            circlator v1.5.3; Pilon v1.18; SPAdes v3.11.1; A5-miseq v20140604
            Genome Coverage       :: 400x
            Sequencing Technology :: Sequel; NextSeq 500
            ##Genome-Assembly-Data-END##
            Annotated by DFAST v.1.2.3 (https://dfast.nig.ac.jp).
FEATURES             Location/Qualifiers
     source          1..5943
                     /organism="Enterobacter cloacae"
                     /mol_type="genomic DNA"
                     /strain="WP8-W18-CRE-04"
                     /isolation_source="wastewater treatment plant effluent"
                     /db_xref="taxon:550"
                     /plasmid="pWP8-W18-CRE-04_5"
                     /geo_loc_name="Japan:Tokyo"
                     /lat_lon="35.6316 N 139.7460 E"
                     /collection_date="2018-02-13"
     gene            101..745
                     /locus_tag="WP8W18C04_P50010"
     CDS             101..745
                     /locus_tag="WP8W18C04_P50010"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BBT47445.1"
                     /translation="MDKDQVEFVVKSVIRTDGYLNYANTKSTILLTLSSATLATLAVNA
                     GKLVPDKMNDISNILFISLISISFLLILVSMYFSIQSINPYLKASEIENTMSFVDITAY
                     NKNAKEYSQKIASKTNEELCIELASLNFNLSNGLIGKYQKQKKSIKYFMASIISATFSI
                     VMPNLIEALKINQHSQSLTVLLLLVVISLIGVICVMSLAIFMILALKWKNK"
                     /name="hypothetical protein"
     gene            742..2148
                     /locus_tag="WP8W18C04_P50020"
     CDS             742..2148
                     /locus_tag="WP8W18C04_P50020"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BBT47446.1"
                     /translation="MKIDDLYSNLNKDINSISKRKKTNARAFEKMAFDQNAESDLSMLS
                     GAEDYSSSVPSFESYLSESFNRTAVERDSLTPISDKNSILKLRPLFGKSENHSSLTIGC
                     HPDFIHLEEPNSKPESGYAVTMFIDIVGSTKLGVLYSPNDVFLFKNAVITGAIETITAF
                     DGHVHRIMGDAVMAFFRSKDLEDSVHSLNSAIDAINCASYLIEVMDKIVMPQIKEDGLD
                     KVGIRIGIDLGMKDWVLWSNYGIPGINEITATSFYVDIASKLQHKAPTNSIMIGDTLAK
                     ELGLIESDFIKIKKKKKNEEFVEEPYVINVTSNGNRLKYRQYLLDNKKYFSCLPHGMSE
                     SEIKLVVRHGPSKEITSLSEYMNCSKVIPKNNHVNFDVEYTNSSIKSTDNVEFKFEVIN
                     TGKDAKEKNKEGREYGNHESMVKANKLGGKYTASHWEETKYKGLHHMFISVYINGQQYT
                     EKKKFSLFIA"
                     /name="hypothetical protein"
     gene            complement(2138..3052)
                     /locus_tag="WP8W18C04_P50030"
     CDS             complement(2138..3052)
                     /locus_tag="WP8W18C04_P50030"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BBT47447.1"
                     /translation="MNNAVLELFNDRLPHKPYFSDDLHFGVRIAGKERAILAKYIQFNQ
                     PHAMFWLGFDVDRAGAAIDWSDRNAPAPTLTIINPENGHAHLLYALETPVRTAPDGKMK
                     PLRYAAAVENALRKKLEADAGYSGLICKNPNHGHWKIAVWQPELYTLDWLADFLDLNAA
                     SDKDIVADYGLGRNCTLFDKTRKWAYRAIRQGWPEYDQWLQACYERASVYNLQFAVPLD
                     ENEVKGIAKSIAKWTSIKITEKSFEAFVLATHSSDIQSKRGRVSKGGGRPKKNDSLDLL
                     RPWDDLGISRRKYFYMKKEKTKL"
                     /name="hypothetical protein"
     gene            complement(3245..3529)
                     /locus_tag="WP8W18C04_P50040"
     CDS             complement(3245..3529)
                     /locus_tag="WP8W18C04_P50040"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /inference="similar to AA sequence:RefSeq:WP_000007436.1"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="BBT47448.1"
                     /translation="MSFFDELKASLEEAVEIKNGVREPARVTRYELADVKAIRAQLNVS
                     QSEMAKVLGTSLDTIKSWETGRRNPTGLAAKVLATIQANPKFFQELAAH"
                     /name="transcriptional regulator"
     gene            complement(3531..3863)
                     /locus_tag="WP8W18C04_P50050"
     CDS             complement(3531..3863)
                     /locus_tag="WP8W18C04_P50050"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /inference="similar to AA sequence:RefSeq:WP_000513548.1"
                     /codon_start=1
                     /transl_table=11
                     /product="toxin RelE"
                     /protein_id="BBT47449.1"
                     /translation="MSHAIEFIETPMFTRQIKQIATDEELRELQKTLIESPDKGDLIQK
                     TGGLRKIRMATGNQGKSGSVRVIYFLATAEVIYLVMAYPKSTKDSLTDAEKAALKTLTQ
                     QLKDEV"
                     /name="toxin RelE"
     gene            complement(4019..4252)
                     /locus_tag="WP8W18C04_P50060"
     CDS             complement(4019..4252)
                     /locus_tag="WP8W18C04_P50060"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BBT47450.1"
                     /translation="MTEMEKQLLSALESLQTSYEQQQQAWQDSYSSLQHMFEATSQALT
                     HSDRVCQHLSSQVDSLRAQVESLSRNVSRLMR"
                     /name="hypothetical protein"
     gene            complement(4313..4798)
                     /locus_tag="WP8W18C04_P50070"
     CDS             complement(4313..4798)
                     /locus_tag="WP8W18C04_P50070"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BBT47451.1"
                     /translation="MSEILNLAKGFEEKSKQQAQDTEQSVKREFARLETSISAELQSSS
                     QSISNAIREQNGRLTGMIRTSVSAAMAWVFLCFALLIGIMGGIIWFQGTIITSRLSEMD
                     SYSQQLANLKAKSGAGVTIYSDDTRRNTYLVVLPKQAHGVETYTSTTDNTVVKYTAK"
                     /name="hypothetical protein"
     gene            complement(5476..5802)
                     /locus_tag="WP8W18C04_P50080"
     CDS             complement(5476..5802)
                     /locus_tag="WP8W18C04_P50080"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BBT47452.1"
                     /translation="MEKRTKEIKIRLTETEHQRLLDRCDRTHLAEWLRQLGLGEHTSRK
                     RRVPEVAPELLRQVSGIGNNLNQIARRLNQTDSLTPSERASLLVVLTSLDRQLGDLLEQ
                     NRDR"
                     /name="hypothetical protein"
     Typing          complement(3928..5486)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="92.337"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tagtgtgaat gtgtaaggta gtatataagt gtcacataag gaaagaaaaa aaggctgtta
       61 tctgatatag gttgttttag tgcaaatgtg agggttgggt atggataagg atcaggttga
      121 atttgtagta aaatcggtga taagaacaga tgggtatctg aactatgcaa acactaaatc
      181 aacaatactt ttaacattat cctccgcaac gcttgcaacg ctggctgtaa acgcagggaa
      241 actagtgcca gataaaatga atgatatttc aaacatttta tttatatcgt taatatcaat
      301 ttctttttta ttgatattag tgtcgatgta cttttcaatt cagtctataa acccatattt
      361 gaaggctagt gaaatagaga acacaatgtc attcgttgac attacagcat acaataaaaa
      421 cgccaaggag tattcgcaaa aaatagcatc aaaaaccaac gaggaattgt gcattgaact
      481 agcatcatta aacttcaacc tatcaaatgg tttgataggg aagtatcaaa aacaaaagaa
      541 atcaataaaa tacttcatgg catctataat atcagcaaca ttttcgatag taatgcctaa
      601 tttgatcgaa gcgttaaaga taaatcagca tagtcagtca ttaaccgtgt tgttgctact
      661 ggtggtaatc tcactcattg gagtgatatg tgtaatgtca ttagccatat tcatgattct
      721 agcattaaaa tggaaaaaca aatgaaaata gatgatttgt actcaaatct taataaagat
      781 ataaactcaa tatctaaaag aaaaaaaaca aatgcacggg catttgaaaa aatggcattt
      841 gaccaaaatg cagaaagcga cttatccatg ttatcaggag cagaggacta tagttcaagc
      901 gttccgagtt ttgaaagtta cttatcagag tcatttaatc gaaccgccgt tgagagagat
      961 tccttaacac cgatatcaga taaaaactca atattaaagc taagaccatt gtttggtaaa
     1021 agtgagaatc atagctcgct aaccattgga tgccatccag atttcataca cttggaggaa
     1081 ccaaattcaa agccagaatc tggatatgca gtaacaatgt ttatagatat cgtaggttct
     1141 actaaattag gcgttttata ctcaccgaat gatgtatttc tgttcaagaa tgcagtaata
     1201 acaggagcca tcgagacaat aacagccttt gacggtcatg tgcataggat tatgggggat
     1261 gctgtaatgg catttttcag aagcaaagac ctagaggata gcgttcatag cttaaacagc
     1321 gctatagatg ccataaattg cgcatcatat ctcattgaag ttatggataa aattgtcatg
     1381 ccgcagataa aagaggatgg gctagataaa gtcggcatta gaattggtat agatttaggg
     1441 atgaaagatt gggttttatg gagtaattat ggaatacctg gcataaacga aatcactgca
     1501 acatctttct atgttgatat tgcgtcaaaa ctacagcaca aagctccaac aaactcgata
     1561 atgattggag atactttagc aaaagaactt gggttaatag aaagtgattt tataaaaata
     1621 aaaaagaaaa agaagaatga agagtttgta gaggaaccat atgttattaa cgtcactagc
     1681 aatggaaata gattgaaata taggcagtat ctattagata acaagaaata tttttcttgc
     1741 ctacctcatg ggatgagtga atcagaaata aagctagtag ttcgacatgg accatcaaaa
     1801 gaaataacaa gtcttagtga gtacatgaac tgttcaaagg tgataccgaa aaacaatcac
     1861 gtgaatttcg atgttgaata tacaaactca agcataaaaa gcaccgacaa cgttgaattt
     1921 aaatttgaag ttatcaatac agggaaagat gcaaaagaaa aaaacaaaga aggacgtgaa
     1981 tacggaaacc atgagagcat ggtaaaagcc aataaattag gtggcaagta taccgcaagt
     2041 cactgggaag aaacaaagta caaaggattg caccatatgt tcataagtgt ttacatcaat
     2101 gggcagcagt acacagaaaa aaagaaattc agcttattta tagcttagtt ttttcttttt
     2161 tcatgtaaaa atacttcctc ctactaatac ccaaatcatc ccaagggcgt aataaatcta
     2221 aagaatcatt ttttttagga cgccctccac ctttgctaac cctccctctt ttggattgaa
     2281 tatcggatga atgagtagcc aaaacaaaag cttcgaatga tttttccgtt atttttatac
     2341 ttgtccattt agctatgctt ttagctattc cctttacctc attttcatcc agaggcacag
     2401 caaattgcag gttgtagacg ctagctcgtt cgtagcaggc ttgtagccac tggtcatact
     2461 ccggccagcc ctgacggatg gcgcggtatg cccatttgcg ggttttgtcg aacagggtgc
     2521 agttacggcc taagccgtag tcagcaacga tgtctttgtc gctcgctgca ttcaggtcga
     2581 gaaaatcagc cagccagtcc agcgtgtaga gttccggctg ccatacggca attttccagt
     2641 ggccgtggtt cggatttttg cagattagtc ccgaataccc cgcgtcagcc tctaattttt
     2701 tacgcagcgc gttctccacg gcggcggcgt agcgcagcgg cttcattttt ccgtcaggcg
     2761 cggtgcgtac cggggtttcc agcgcgtaca gcaggtgtgc gtgtccgttt tccgggttga
     2821 tgatggtcag cgtgggcgca ggcgcgttcc ggtcactcca gtcgatggca gcaccggcgc
     2881 gatcgacgtc gaaacccagc cagaacatgg cgtgaggctg gttgaactgg atgtatttcg
     2941 ccagaatggc gcgttcctta ccggcaatgc gtacaccgaa gtgtaaatca tccgagaagt
     3001 acggcttgtg cggtaagcgg tcgttgaaaa gttcgagtac tgcgttattc aattcgccct
     3061 gcgcttatgg cgggctgttg tctggcacgt ttcatgtgct aatatccttt caaggtttcg
     3121 acctggccct ggttatcatg tccgctcgcc aaagtagagc atgatttccg gggctttgtt
     3181 ttttctgttg ccgttaaatt acacgcgaac ggcctcgcct gtcgaatcct tctgtcacaa
     3241 cggcttagtg cgccgccagc tcctggaaga atttcgggtt cgcctggatg gttgccagca
     3301 cctttgctgc cagcccggtc gggttacggc gtccggtttc ccagctcttg atggtatcga
     3361 gactggttcc cagcactttc gccatctcgc tctgtgaaac gttcagctgt gccctgatgg
     3421 ccttcacgtc tgccagctcg taacgggtga cgcgggcagg ctctctgacg ccgtttttga
     3481 tttcaacggc ttcttccagc gatgctttca gctcgtcaaa aaaactcatg tcacacctcg
     3541 tctttcagtt gttgggtcag cgtcttcagc gcggcttttt ccgcgtccgt caggctgtct
     3601 ttggtgcttt tcgggtaggc catcaccaga tagatcacct cggcggtggc cagaaagtaa
     3661 atcaccctca cgctgccgct ttttccctgg ttgcccgtcg ccatcctgat tttgcgcagc
     3721 ccgccggttt tctgaatgag atcgcctttg tccggcgact caatcagcgt cttttgcagc
     3781 tccctgagtt cttcatccgt ggcgatctgt tttatctgac gggtaaacat cggggtctcg
     3841 ataaactcaa tcgcgtgact cacggctgtc tcctgttttc aatggagtac atagtacact
     3901 taattctgat tccggaaaag ccgtcacata gagggaccat agtagcgggt cggttttttc
     3961 tgcggctctg gctgctgctc acgcagcttc tcggcgtagg cgcggtcgtg ctgcctgatc
     4021 agcgcatcaa gcggctgaca ttcctgctca agctctcgac ctgcgcgcgc aagctgtcca
     4081 cctgactgct caagtgctga cacaccctgt cgctgtgcgt tagcgcctgc gaggtagcct
     4141 cgaacatgtg ctgcaagctg ctgtagctgt cctgccacgc ctgctgctgt tgctcgtagc
     4201 ttgtctgtaa gctctctaaa gcgctcagaa gctgtttttc catctcggtc atggttttgg
     4261 tcgtcctcag tgttaaaaac gcgctgtggc gtttcagggc gctctgacgg ggttatttcg
     4321 ctgtgtattt caccacggtg ttgtcggtcg tcgacgtgta ggtttccacg ccgtgcgcct
     4381 gcttcggcag cactaccaga taggtgttcc ggcgcgtgtc gtcgctgtaa atcgtcacgc
     4441 ccgcgccgct tttcgctttc aggttcgcca gctgctggct gtagctgtcc atttcgctca
     4501 ggcgcgaggt gatgatcgtg ccctgaaacc agattatccc ccccatgatg ccgatcaaca
     4561 gggcgaagca gaggaatacc cacgccatcg ccgctgaaac ggaggttctg atcatgccag
     4621 tcagcctgcc gttctgctcg cggatagcgt tgctgatgct ctgcgagctt gattgcagtt
     4681 ccgcgctgat agacgtctcc agcctcgcga actcgcgctt cacgctctgc tcggtatcct
     4741 gcgcctgctg cttcgatttc tcctcgaagc ccttcgccaa atttaaaatc tcgctcataa
     4801 atcattcctt tcagccggat attccggccg ccgtccggat cggcaatgct gatgctgctt
     4861 ttcgtttccc gcgctacggt caggccgtag ctttccagct gcgtcaccac gtcctgccgg
     4921 ctgcggatca cgccctgctg catcaggttc atcaggccgg cggtgattct ctctgccgcc
     4981 tgctgtttgt cacggggcag atcgctggcc tgtgtcagcg ggcggcggta ggtcgggtcg
     5041 ttcgggtcac gcagccccag ccggtcgttg gtcagggtct gccaggcgtt gacgcgggga
     5101 cggtcggcgc ggtcaaagta gggttgcagt cgtttgccac tctgcaattc gatgttcggg
     5161 atgacaaaat tcagctcaag ccgtcccttg tcgcggtgct ccacccacag gcaggcgtac
     5221 tggtcacggt cgagaccagg cagcagcgtg tactcccatt cgtccatcag gcgtgatttc
     5281 tcggcatctg caatgtccgg ctcctgaaat gacagcacgc cggaagtata ggcgcgggca
     5341 aagtcgcagc cgtctatcaa ctcgcggacg tattcaggat tgccgcgcag tacgctggcc
     5401 tgttcgcgct gccggtcttt tccaagcagg taatccacag gaccactgcc ggaacctgca
     5461 ccgcgtgagt gaattttaac gatcacggtt ctgctccagc aggtcgccaa gctgacggtc
     5521 aaggctggtc agtacgacga gcaacgaggc acgttcagaa ggcgtcaggg aatcagtctg
     5581 gttcaggcgg cgggcaatct ggttcagatt gttgccaatc ccgctgacct gtcgaagcag
     5641 ttcgggcgcc acctcaggca cacggcgctt gcgtgaggtg tgttcaccca gacctagctg
     5701 acgcagccac tccgcaaggt gagtccggtc acagcgatca agcagtcgct gatgctcggt
     5761 ttcggtgagc cggattttga tctctttcgt tcgcttttcc ataggctccg ctgagaaaaa
     5821 attcgcacca ctgtgagaat ttttcgcagt ggatgccggg ggtttcgggg ggcggcgagc
     5881 cccccgaacc agtcacagac ggcacctcat agaggggacg ctgtgtgtac tggcttaaag
     5941 agg
//
