LOCUS       AP022232                9495 bp    DNA     circular BCT 22-JUL-2020
DEFINITION  Enterobacter cloacae plasmid pWP8-W18-CRE-04_4 DNA, complete genome,
            strain: WP8-W18-CRE-04.
ACCESSION   AP022232
VERSION     AP022232.1
DBLINK      BioProject: PRJDB6962
            BioSample: SAMD00194608
            Sequence Read Archive: DRR199444
KEYWORDS    .
SOURCE      Enterobacter cloacae
  ORGANISM  Enterobacter cloacae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1
  AUTHORS   Sekizuka,T., Itokawa,K., Yatsu,K., Inamine,Y. and Kuroda,M.
  TITLE     complete genome sequences of Enterobacter cloacae str.
            WP8-W18-CRE-04 isolated from wastewater treatment plant effluent
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9495)
  AUTHORS   Sekizuka,T., Itokawa,K., Yatsu,K., Inamine,Y. and Kuroda,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-DEC-2019) Contact:Tsuyoshi Sekizuka National Institute
            of Infectious Diseases, Pathogen Genomics Center; 1-23-1 Toyama,
            Shinjuku-ku, Tokyo 162-8640, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: flye v2.5; minimap v0.2-r124; racon v1.1.0;
            circlator v1.5.3; Pilon v1.18; SPAdes v3.11.1; A5-miseq v20140604
            Genome Coverage       :: 400x
            Sequencing Technology :: Sequel; NextSeq 500
            ##Genome-Assembly-Data-END##
            Annotated by DFAST v.1.2.3 (https://dfast.nig.ac.jp).
FEATURES             Location/Qualifiers
     source          1..9495
                     /organism="Enterobacter cloacae"
                     /mol_type="genomic DNA"
                     /strain="WP8-W18-CRE-04"
                     /isolation_source="wastewater treatment plant effluent"
                     /db_xref="taxon:550"
                     /plasmid="pWP8-W18-CRE-04_4"
                     /geo_loc_name="Japan:Tokyo"
                     /lat_lon="35.6316 N 139.7460 E"
                     /collection_date="2018-02-13"
     gene            101..325
                     /locus_tag="WP8W18C04_P40010"
     CDS             101..325
                     /locus_tag="WP8W18C04_P40010"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BBT47434.1"
                     /translation="MAFTIDDTVNQGSPPGYAPAANVLIVPNYSAGHFLPDGSQQIKTL
                     SATGHTMAPPVSFDFPAPEIFTQDQNARF"
                     /name="hypothetical protein"
     gene            506..1099
                     /locus_tag="WP8W18C04_P40020"
     CDS             506..1099
                     /locus_tag="WP8W18C04_P40020"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BBT47435.1"
                     /translation="MKRTALVIALTLATTSAMAAPVFYTHPWGNSVEANLNGKTALLND
                     HQDQITQNRVDSESRDTLLGDRITSNTSALTTKVDKQVFADDQARQDAALKSETTTRTS
                     QVAKLQSGVDQAQATGDYANSRIDVANQNIEANRQASIATLADHDTRITSNTSALTTKV
                     DKQVFADDQARQDAALKSETTTRTSQVAKHFTGS"
                     /name="hypothetical protein"
     gene            1113..1811
                     /locus_tag="WP8W18C04_P40030"
     CDS             1113..1811
                     /locus_tag="WP8W18C04_P40030"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BBT47436.1"
                     /translation="MDQAQATGDYANSRIDVANQNIEANRQASIATLADHDTRITSNTS
                     ALTTKVDKQVFADDQARQDAALKSETTTRTSQVAKLQSGVDQAQATGDYANSRIDVANQ
                     NIEANRQASIATNKRVAANTAQLANHEQRITTLEQNTSARFSDLNSKIDDNRKRASAGI
                     AGVAAMANIPQVLEHQTFAIGAGAGNTDGESALAVGFSARASQNTVVKASVSNDTQHNF
                     VVGAGVAFGW"
                     /name="hypothetical protein"
     gene            complement(2076..2357)
                     /locus_tag="WP8W18C04_P40040"
     CDS             complement(2076..2357)
                     /locus_tag="WP8W18C04_P40040"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BBT47437.1"
                     /translation="MNALSALLTKIELASPTQRDKGTTFENLCVQYFLHEPKYAELYSD
                     VLSYGGWVSQYGETVGVTKKKDDGIDLVAVTKTGEFHAIQCKNYNQGV"
                     /name="hypothetical protein"
     gene            complement(2470..3054)
                     /locus_tag="WP8W18C04_P40050"
     CDS             complement(2470..3054)
                     /locus_tag="WP8W18C04_P40050"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BBT47438.1"
                     /translation="MSDEIKQIAALIGHHQALEKRVTSLTEQFQAASSQLQQQSETLSR
                     VIRELDSASGNMTDTVRKSVSSALTLVEKELKQAGLAQQKPATEALNQAADTAKAMIHE
                     MRREMSRYTWKSAIYLVLTIFFVLASCVTAFTWFMNDGYSQIAEMQQMEAVWQKKAPLA
                     DISRCDGKPCVKVDTRTTYGDKENTWMIIKK"
                     /name="hypothetical protein"
     gene            complement(3067..4176)
                     /locus_tag="WP8W18C04_P40060"
     CDS             complement(3067..4176)
                     /locus_tag="WP8W18C04_P40060"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BBT47439.1"
                     /translation="MASYHLSVKTGGKGSASPHADYISRDGKYAREKDSDLEHKESGNM
                     PAWAAHKPSEFWKAADTSERANGCTYREIEIALPRELKPEQRLELVRDFVQQEIGDRHA
                     YQFAIHNPKAAIAGGEQPHAHIMFSERINDGIHRDPEQYFKRANTKEPERGGAKKARFG
                     ETPTERKEYLVAQRERWADLQNKHLERYQHADRVDSRSLKAQGIDREPERHLGAGQVQR
                     FDTEQLQSILARREAERQAQQSRDERDCVIDVTTSLREAIHERDDASVKPAQQPEPEVA
                     AGITFDFEKEPDKLNALVSDAMKDVQEEIDLQSLVNDAMAEFQGIHQEMERQRERERLA
                     EKQRQQEKERQRLAEQIRQKPDKGWSFSR"
                     /name="hypothetical protein"
     gene            4340..4807
                     /locus_tag="WP8W18C04_P40070"
     CDS             4340..4807
                     /locus_tag="WP8W18C04_P40070"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BBT47440.1"
                     /translation="MNKSEKQEKWAADRVQYIRGLKSPNEQQKLMLILTDKADKTAQDI
                     KTLSLLMKAEQAAEKAQEARAKVMNLIQAEKRAEARAARKARDHALYQSAGLLILAGLV
                     DSQTGKPVDDTAALLGALASLNDLSRDNPKWSDWKIRGQELLNSKKSDSSA"
                     /name="hypothetical protein"
     gene            5402..6358
                     /gene="repA"
                     /locus_tag="WP8W18C04_P40080"
     CDS             5402..6358
                     /gene="repA"
                     /locus_tag="WP8W18C04_P40080"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /inference="similar to AA sequence:RefSeq:WP_001293214.1"
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="BBT47441.1"
                     /translation="MAELVVFKANELAVSRYDLTEHETKLILFCVAKLNPTLETPDETE
                     RTVKFSCSEYSSFMGISYENAWGRLNTSTRELFKRSIELIYPTGAVSMRVFNWAEYAEF
                     NRETQEVTLVFSKYIIPLLFHLKRFIKYNLEHVKSFENKYSMRIYEWLLKELTQRKTHK
                     ANISISIDEFKFMLMLEKSYPEYKLLNHWVLKPISKDLNTYSNIKLSIDKRGRPADTLI
                     FQVELDKQIDLVTELAKEPISKKEDNSIRLTPENRLHEGLKTTLHDALTAKIQLTGFEA
                     KFLSDMQSKYDLNGSFTWLTQKQRATLEKILAKYGRN"
                     /name="repA"
     gene            6366..6815
                     /locus_tag="WP8W18C04_P40090"
     CDS             6366..6815
                     /locus_tag="WP8W18C04_P40090"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BBT47442.1"
                     /translation="MALVSISEAARLTGKSRTTVQSYIKQGKLTKCTDSGGASKLDTSE
                     LLRVFGSFTGQQVGSEQSGSTVQHLAGDFVQLPDQNLLHENYALKAEIELVKALLREKE
                     LLLDEKDKRNEDLKHALLLIESKLPNTSEPVTPHVVKKSWQFWKK"
                     /name="hypothetical protein"
     gene            7073..7705
                     /locus_tag="WP8W18C04_P40100"
     CDS             7073..7705
                     /locus_tag="WP8W18C04_P40100"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BBT47443.1"
                     /translation="MLRYELTPNNAGFILWGDSEALNELHELIHYIVDESPLIKVKDGF
                     MLSLAYDIRKAREGNRRVEQHQYDQHDTYKLYGVELLWPLVLVQSSILRNSMGYIQTDK
                     NQLSVMYAFEYLIESALTESERTTSNDIMLTVKYASDSDFNFIEDNIDSRCCYFISLSP
                     EQRKKQLISIVRSFHSLWGKYAREKQDIKMLNEMNNTSWVWPDNINW"
                     /name="hypothetical protein"
     gene            complement(7734..9137)
                     /locus_tag="WP8W18C04_P40110"
     CDS             complement(7734..9137)
                     /locus_tag="WP8W18C04_P40110"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /inference="similar to AA sequence:RefSeq:WP_000974326.1"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="BBT47444.1"
                     /translation="MSAESSGVFTLKEINRIKIIQDVIERRITTRRAAEHLGISDRQCR
                     RLLARYREGGPLGMASRRCGMRGNRQLPPGLADQALELIKTRYADFGPTLAREKLEELH
                     GLFLGKETVRRIMVRAGLWVPRKQRAARIPQPRYRRPCTGELIQIDGCDHDWFEGRGPA
                     CTALVYVDDATSKLMELLFVKSESTFSYFEATRRYIDKHGKPLALYSDKAGVFRVNNKH
                     ATGGDGHTQFGRAMHELNIQTICAETSPAKGRVERAHLTLQDRLVKELRLQGICSMEAA
                     NDFAEAYMADYNRRFGKVPRHDFDVHRAVEHDEDLGLIFTVREKRKVSKSLTIQYDKML
                     YLIENSELSRRAIGKYIDVYHYPDGRKELRLNGTLLPYSTYDRLSEIDQGAIVDNKRLG
                     RTLEFISLVQSKRDNTRSQSIPAGDGPSRRRPKQEGKKSQRSLDNDDMLEALKQLQSRS
                     EDIFGKRAR"
                     /name="transposase"
     Typing          5403..6353
                     /name="rep_cluster_1155"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="80.376"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          complement(3071..4176)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="89.702"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tgttttccga cggaaatttc tgggattccg gcttacaccc ccaactcccg caagtagtga
       61 cggcatactg gagccctcag acataacgag ggctctactt atggcattca ctatcgacga
      121 cacggtaaac caaggctccc caccgggtta cgcgccagcg gcaaacgtgc tgatcgtccc
      181 gaactacagc gccggacact tcctgccaga tggctcacag cagattaaaa cgctgagcgc
      241 taccgggcac acaatggcac cgcctgtatc gtttgacttc ccggcacctg aaatcttcac
      301 gcaagaccag aacgcccgct tttaatattg acccccaaag caccgggggt taaataaata
      361 ccctttccaa tcagtcacct gaatcattcc cgcaaataaa tcataaaaaa gtatcattgc
      421 gctatctctg acgcgtcacc tatgacgtat attctcctcg tcaacacaaa caaaaccgat
      481 aaaccaccaa atgatgaggc tttacatgaa acgaactgct ttggtaattg cgcttacgct
      541 ggctaccacc tccgcaatgg ctgcgcctgt gttttacact catccatggg gtaacagcgt
      601 cgaagccaac ctgaacggca aaaccgctct cctgaacgac caccaagacc agatcacgca
      661 aaaccgcgtg gattctgaaa gccgcgatac cctgctgggc gaccgcatca ccagcaatac
      721 cagtgcgttg actaccaaag tcgataaaca ggtatttgct gatgaccaag cccgtcagga
      781 tgctgcgctg aaaagcgaaa caaccacgcg cacttcacag gtagctaaac tgcaatccgg
      841 cgtggatcag gctcaggcta ctggtgatta cgctaactct cgcattgatg ttgccaatca
      901 gaacattgaa gcgaaccgcc aggcatccat cgcaaccctg gcggatcacg atacccgcat
      961 caccagcaat accagtgcgt tgactaccaa agtcgataaa caggtatttg ctgatgacca
     1021 agcccgtcag gatgctgcgc tgaaaagcga aacaaccacg cgcacttcac aggtagctaa
     1081 acacttcaca ggtagctaaa ctgcaatccg gcgtggatca ggctcaggct actggtgatt
     1141 acgctaactc tcgcattgat gttgccaatc agaacattga agcgaaccgc caggcatcca
     1201 tcgcaaccct ggcggatcac gatacccgca tcaccagcaa taccagtgcg ttgactacca
     1261 aagtcgataa acaggtattt gctgatgacc aagcccgtca ggatgctgcg ctgaaaagcg
     1321 aaacaaccac gcgcacttca caggtagcta aactgcaatc cggcgtggat caggctcagg
     1381 ctactggtga ttacgctaac tctcgcattg atgttgccaa tcagaacatt gaagcgaacc
     1441 gccaggcatc catcgcaacc aataagcgcg tcgcggctaa cacggcacag ctggctaacc
     1501 atgagcaacg catcaccacg ctggagcaga acaccagtgc gcgtttctct gacctgaata
     1561 gcaagattga tgacaaccgc aaacgtgcgt cagcaggtat cgcaggcgtc gcggcaatgg
     1621 cgaatatccc gcaggtactg gagcaccaga cctttgcaat cggcgctggc gctggtaaca
     1681 ccgatggcga gtctgcgctg gctgtaggct tctccgctcg cgcttctcag aacaccgttg
     1741 tgaaggcgtc cgtatcgaac gacacccagc ataactttgt ggtaggcgct ggcgtggcat
     1801 ttggatggta agaaaatcag caagaaccaa aggggctacg gcccctttcc cactgtctgg
     1861 ggttgtaagc ccaaacccca gatttttccg tctggccagc aaagccagga ctggcgaggg
     1921 ttgtcggtaa acagttgatt tccggcaaca acgctgcaaa gccccaaacg gcccgattga
     1981 gctgccaaaa catgcacgac aaaccatatc ggatagcaaa acactcgaaa atctctcgga
     2041 tgatgttttc cgacggaaat ttctgggatt ccggcttaca ccccctgatt gtagttttta
     2101 cactgaatgg catgaaattc acccgtttta gttacggcga ccaaatcaat gccatcatct
     2161 tttttcttcg tgaccccaac ggtttcaccg tactgagaaa cccatccgcc atagctcagc
     2221 acatcgctat acagctcggc atatttaggt tcatggagga aatactgcac acaaaggttt
     2281 tcaaaggttg tacctttgtc acgttgtgta ggtgaagcca gttctatctt tgtcagcaat
     2341 gcagaaagtg cattcatcgt tgatatcctt aatgtccttt atttgctggg gttatcagat
     2401 ccccccgaca cgtttaatta atgctttctc cgccagagat cgacgcacag cgttctgtgc
     2461 tccatgatgt tatttcttaa taatcatcca ggtattctct ttatcaccat acgtagtgcg
     2521 agtgtccacc ttaacgcagg gctttccgtc acagcgcgat atgtcagcca gcggggcttt
     2581 cttctgccag accgcttcca tctgctgcat ttcagcaatc tggctgtacc cgtcattcat
     2641 aaaccacgta aatgccgtca cgcaggaagc caggacgaag aatatcgtca gtacaagata
     2701 aatcgcggat ttccacgtat agcgtgacat ctcacgacgc atttcatgga tcatcgcttt
     2761 cgccgtatcg gcagcctgat tcagcgcttc tgtcgccggt ttctgctgtg ctaatccggc
     2821 ttgtttcagt tctttctcaa ccagagtgag cgcggaactc accgatttcc tgacggtgtc
     2881 agtcatatta ccggacgcgc tgtccagctc acgaatgacc ctgctcagcg tttcactttg
     2941 ctgctgtaat tgtgatgagg cggcctgaaa ctgttctgtc agagaagtaa cacgcttttc
     3001 cagcgcctga tgatgcccga taagggcggc aatttgttta atttcgtcgc tcatacaaaa
     3061 tcctgtctat cgtgagaatg accagccttt atccggcttc tgtcgtatct gttcggcgag
     3121 tcgctgtcgt tctttctcct gctgacgctg tttttccgcc agacgttcgc gctctctctg
     3181 cctttccatc tcctgatgta tcccctggaa ctccgccatc gcatcgttaa caagggactg
     3241 aagatcgatt tcttcctgta cgtccttcat ggcatcactg accagtgcgt tcagcttgtc
     3301 aggctctttt tcaaaatcga aggtgatgcc ggcagcgact tccggctcag gttgctgcgc
     3361 cggcttaacg gacgcatcat cccgttcgtg gatggcttcc cgcagcgagg tggtaacatc
     3421 aatgacgcag tcccgttcat cacgggactg ctgcgcctgt ctttccgctt ccctgcgcgc
     3481 aagaatggac tgtaactgtt cagtatcgaa ccgctgaacc tgacccgcgc ccagatgccg
     3541 ctcaggctca cggtcaatgc cctgcgcctt cagggaacgg gaatcaaccc ggtcagcgtg
     3601 ctgataccgt tcaaggtgct tattctggag gtcagcccag cgttcccgct gggcaacaag
     3661 gtattctttg cgttcggtcg gtgtttcccc gaaacgtgcc ttttttgcgc caccgcgctc
     3721 cggctctttg gtgttagccc gtttaaaata ctgctcaggg tcacggtgaa taccgtcatt
     3781 aatgcgttca gagaacatga tatgggcgtg gggctgctcg ccaccggcta tcgctgcttt
     3841 cggattatgg atagcgaact gataggcatg gcggtcgcca atttcctgtt ggacaaaatc
     3901 gcggacaagc tcaagacgtt gttcgggttt taactcacgc ggcagggcaa tctcgatttc
     3961 acggtaggta cagccgttgg cacgttcaga cgtgtcagcg gctttccaga actcggacgg
     4021 tttatgcgct gcccacgcag gcatattgcc ggactcctta tgctcaaggt cggagtcttt
     4081 ttcacgggca tactttccat cacgcgaaat gtaatcggca tggggagagg cactgccttt
     4141 cccgccggtt tttacgctga gatgataaga tgccatcgtg ttttatcccg ctgaaggcgc
     4201 gcaccgtttc tgaacaaagt gaagaaacgt ctaagtgcgc actgataata aaaagcttat
     4261 cagtgattct acctgcattt gacctcggcc gccattcctg accataattt ccacatcaac
     4321 attcagagga taaatgggca tgaataaaag cgaaaaacag gaaaaatggg cagcagacag
     4381 agtgcagtac attcgcggct taaagtcgcc gaatgaacag cagaaactca tgctgatact
     4441 gacggataaa gcggataaaa cagcgcagga tatcaaaacg ctgtccctgc tgatgaaggc
     4501 tgaacaggca gcagaaaaag cgcaggaagc cagagcaaaa gtcatgaatc tgatacaggc
     4561 cgaaaaacgg gcagaagcca gagccgcccg taaagcccgt gaccatgcgc tgtaccagtc
     4621 agccggactg cttatcctgg cgggtctggt tgacagccag acggggaagc ctgttgatga
     4681 taccgctgcc ttgctgggtg cattagccag tctgaatgac ctgtcacggg ataatccgaa
     4741 gtggtcagac tggaaaatca gaggtcagga actgctgaac agcaagaagt ctgacagctc
     4801 tgcatagcag acctgccata aaacgccatg agaagcccgt gacgggcttt tcttgtgtca
     4861 tgggtagatt tcctcgtgtg agtccatgaa aagcgcctgt agcatcattt acccccattc
     4921 cctgccagag ccgtgagcgt agcgaactga atgtcacgaa aaagacagcg actcaggtgt
     4981 ctgatggtcg gagacgaaag gaatattcag cgatttgccc gagcttgcga gggtgctacc
     5041 tcattctttc gtgtttgaag gtatgttttt tggaggagca aacgctgttt gcgacatcct
     5101 tttatcatat cgctcaactg gcactggcgg ttagttatcc acaaagctgg gatctattct
     5161 tttttatctt ttttattctt tctttattct agtgatttta atattttgtt ttataagtaa
     5221 aaaacacatg aaaggttaag tgaaaattag gaaaggttaa gcaaaaatta ggatccccct
     5281 tcaaaaggtt aagtgaaaat taggtaatcc acacaaccac ataaaaggtt atgacaatga
     5341 aattgacata accaagcgga agggttatgt tgttttaaaa tcacaaccaa tggttttagt
     5401 catggctgaa ttggtagtat tcaaagcaaa cgagcttgcg gttagtcgtt atgacctaac
     5461 agagcacgaa acgaaactaa tcctgttttg tgttgcaaag ctaaacccta cattagaaac
     5521 accagatgaa actgaacgaa cggttaaatt ttcatgctcc gaatattctt catttatggg
     5581 gataagttat gagaatgcgt gggggcgatt aaatacatca acacgagagt tatttaagcg
     5641 gtctattgaa ttaatatatc caacaggtgc tgtatctatg cgtgttttta attgggctga
     5701 atatgcagag ttcaataggg aaacacaaga ggtaacttta gtttttagta aatatataat
     5761 cccattgcta tttcacctaa agagattcat taaatacaac ctcgagcatg ttaagtcctt
     5821 tgaaaataaa tactcaatgc gcatttatga atggctgcta aaagaactaa cgcaaagaaa
     5881 aacacataaa gccaatattt cgataagcat tgatgagttt aaattcatgc taatgttaga
     5941 aaaaagctac ccagaatata aattactaaa tcattgggtt ttaaaaccaa tttcaaaaga
     6001 tttaaatacc tatagcaata taaaactatc tattgataag cgcggtcgtc ctgctgatac
     6061 gctgatcttc caagttgaac tggataaaca aattgacctt gtgactgagc tagcaaaaga
     6121 accaatatca aaaaaagaag ataactcaat ccgtttaacg cctgaaaatc gtctgcatga
     6181 ggggctaaaa acaacattac atgatgcttt aactgcgaaa atacaactta ctggatttga
     6241 agctaaattc ctgagtgaca tgcagagtaa gtatgatctc aacggctcat ttacatggct
     6301 gactcaaaaa caacgagcca cgctagagaa aattttagct aagtacggac ggaactgagg
     6361 tatctatggc tttagtatca ataagcgaag cagccagact aactggaaaa agccgcacaa
     6421 cagtacagtc atacattaag cagggaaaac tgaccaagtg cacagatagc ggtggtgctt
     6481 caaaactaga cacatcagag cttctacgtg ttttcggatc atttactggt cagcaggttg
     6541 gcagtgaaca atcaggcagt actgtgcagc atttggcagg cgattttgtg cagttacctg
     6601 accagaattt gctacatgaa aactatgcct taaaagctga aatagaactc gtaaaggcat
     6661 tgctgcgtga gaaagagcta ctactcgatg aaaaagacaa acgcaatgag gatctcaaac
     6721 atgctttgct gctaatcgag tctaaactgc cgaatacatc ggaaccagtg acacctcatg
     6781 tggtgaaaaa atcatggcaa ttctggaaga aatagcgctt tcagccggca aaccggctga
     6841 agccggatct gcgattctga taacaaacta gcaacaccag aacagcccgt ttgcgggcag
     6901 caaaacccgt acttttggac gttccggcgg gtttttgtgg cgagtggtgt tcgggctgtc
     6961 aaggctcaat tgaaatgtcc gttatccagc tcaattaaaa tgaccacttt gatctctcat
     7021 tctctttact gatagacttt cctccgactg aaacaacagg atgattgagc ccatgcttcg
     7081 atacgagtta acgccgaaca atgcaggttt tatactgtgg ggagattcag aagccctgaa
     7141 tgaattacat gaactcattc attacatcgt ggatgaaagc ccactgatta aagttaaaga
     7201 cggatttatg ttatcccttg cctatgatat tcgtaaagca cgggaaggta atcgtcgtgt
     7261 tgagcaacat cagtatgatc aacatgatac atataagctt tatggtgttg agcttttatg
     7321 gcctctggtc ctggtacagt cctcaatact cagaaactca atgggttata ttcagacaga
     7381 caaaaaccag ctgtctgtca tgtatgcctt tgaatacctg atagaatcag cattaacaga
     7441 gtctgagaga acaacgtcga atgatattat gctaacagta aaatatgcat cagactctga
     7501 ttttaatttc attgaggata atattgacag caggtgctgc tattttatca gcctatctcc
     7561 ggagcaaaga aaaaagcagt taatcagtat tgttcgttct tttcattcat tatggggtaa
     7621 gtatgcccgt gaaaagcagg acataaagat gctgaacgaa atgaataata catcatgggt
     7681 ctggccggac aatatcaact ggtgagcaac cactgtccgg ccagtgagta ccatcagcgg
     7741 gctcttttac caaaaatgtc ctctgaacgt gactgaagct gtttgagtgc ttcgagcatg
     7801 tcatcattat ccagtgagcg ctgggatttc ttcccttcct gctttggccg tcgtcgggaa
     7861 gggccatctc cagcgggaat tgactgagag cgcgtgttat cccgcttgct ctgcaccaga
     7921 ctgataaact ccagggttcg gccaagacgc ttgttatcga caatcgcgcc ctggtcgatt
     7981 tctgacagtc ggtcgtaggt agagtaggga agtagcgtac cgttcaggcg cagctctttt
     8041 ctgccatcag gatagtgata cacatcgata tatttaccta ttgcacggcg actcagttcg
     8101 ctgttttcaa tcaggtacaa cattttatca tattgtatcg tcaacgattt tgagacttta
     8161 cgtttttcac gaacagtgaa aataagcccc aggtcctcat catgttctac agcacggtgt
     8221 acgtcaaaat catgtcgcgg tactttgcca aaacggcggt tatagtcagc catataggcc
     8281 tcagcgaagt catttgcagc ctccattgaa caaatgccct gtaaccgcag ctctttgacc
     8341 agacgatcct gtaaagtgag gtgagctcgt tctacacgcc ctttggcggg actggtttct
     8401 gcacagatag tctggatgtt cagttcatgc atggctcgcc caaactgagt atgcccgtct
     8461 ccgcctgtgg cgtgtttatt gttaacacga aaaacaccgg ctttatcgct gtacagtgcc
     8521 agcggtttac catgcttatc gatatagcgc cgcgtggctt cgaagtaaga aaacgtggac
     8581 tccgatttaa caaacaacag ttccatcagt ttgctggttg catcatcaac atagaccagc
     8641 gcggtgcagg ccgggccacg gccttcaaac cagtcgtgat cacagccatc tatttgtatc
     8701 agctcaccag tacacggacg ccggtaccgt ggttgaggga tccttgcggc acgttgttta
     8761 cggggaaccc ataagccagc ccgcaccatg atgcgccgga cagtttcttt gccaagaaac
     8821 agtccgtgga gttcttcgag cttttcacgc gccagagtcg gaccgaaatc agcataacgc
     8881 gtcttgatca gttccagagc ctgatctgcg agcccgggtg gcaactggcg gttaccacgc
     8941 atgccacatc gtctgctggc cataccaagc ggtccgcctt cacggtaacg ggcaagaagt
     9001 ctgcggcatt gcctgtcgct gataccgagg tgctcggccg cacggcgcgt tgtgatgcga
     9061 cgttcaatga cgtcctgtat aatcttgatc cggttgatct ctttcaaagt aaacactcct
     9121 gagctttctg cgctcatgat atgcctcccg gtagtttaaa cggaccagtg aagcttacca
     9181 tagcgcggac atctgaattg agccacaggc ggacattact attgagccat tacacgggcg
     9241 gtgcgcgcaa gatcgcttat gttaaatact acctgtttgg atgcattttt ccgcaactgt
     9301 acccagtgtt ggggttgtaa gcccaaaccc cagatttttc cgtctggcca gcaaagccag
     9361 gactggcgag ggttgtcggt aaacagttga tttccggcaa caacgctgca aagccccaaa
     9421 cggcccgatt gagctgccaa aacatgcacg acaaaccata tcggatagca aaacactcga
     9481 aaatctctcg gatga
//
