LOCUS       AP022231               11175 bp    DNA     circular BCT 22-JUL-2020
DEFINITION  Enterobacter cloacae plasmid pWP8-W18-CRE-04_3 DNA, complete genome,
            strain: WP8-W18-CRE-04.
ACCESSION   AP022231
VERSION     AP022231.1
DBLINK      BioProject: PRJDB6962
            BioSample: SAMD00194608
            Sequence Read Archive: DRR199444
KEYWORDS    .
SOURCE      Enterobacter cloacae
  ORGANISM  Enterobacter cloacae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1
  AUTHORS   Sekizuka,T., Itokawa,K., Yatsu,K., Inamine,Y. and Kuroda,M.
  TITLE     complete genome sequences of Enterobacter cloacae str.
            WP8-W18-CRE-04 isolated from wastewater treatment plant effluent
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11175)
  AUTHORS   Sekizuka,T., Itokawa,K., Yatsu,K., Inamine,Y. and Kuroda,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-DEC-2019) Contact:Tsuyoshi Sekizuka National Institute
            of Infectious Diseases, Pathogen Genomics Center; 1-23-1 Toyama,
            Shinjuku-ku, Tokyo 162-8640, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: flye v2.5; minimap v0.2-r124; racon v1.1.0;
            circlator v1.5.3; Pilon v1.18; SPAdes v3.11.1; A5-miseq v20140604
            Genome Coverage       :: 400x
            Sequencing Technology :: Sequel; NextSeq 500
            ##Genome-Assembly-Data-END##
            Annotated by DFAST v.1.2.3 (https://dfast.nig.ac.jp).
FEATURES             Location/Qualifiers
     source          1..11175
                     /organism="Enterobacter cloacae"
                     /mol_type="genomic DNA"
                     /strain="WP8-W18-CRE-04"
                     /isolation_source="wastewater treatment plant effluent"
                     /db_xref="taxon:550"
                     /plasmid="pWP8-W18-CRE-04_3"
                     /geo_loc_name="Japan:Tokyo"
                     /lat_lon="35.6316 N 139.7460 E"
                     /collection_date="2018-02-13"
     gene            complement(101..2284)
                     /gene="katG"
                     /locus_tag="WP8W18C04_P30010"
     CDS             complement(101..2284)
                     /gene="katG"
                     /locus_tag="WP8W18C04_P30010"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /inference="similar to AA sequence:UniProtKB:Q9KRS6"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase-peroxidase"
                     /protein_id="BBT47426.1"
                     /translation="MENKDASSSGKCPVMHGGATSASMQTMEWWPKALNLDILHQHDSK
                     TNPLGTDFNYREELKKLDVQALKNDLKALMTDSQSWWPADWGHYGGLMIRMAWHSAGTY
                     RIADGRGGGGTGNQRFAPLNSWPDNANLDKARRLLWPIKKKYGNKLSWADLIILAGTMA
                     YESMGLKTFGFAFGREDIWHPEKDTYWGSEKEWLAPSGSAGSRYSGERDLENPLAAVMM
                     GLIYVNPEGVDGKPDPLKTAHDVRITFARMAMDDEETVALTGGGHTVGKTHGNGSAANL
                     GPAPEGAELEEQGLGWQNHTTRGIGRDTVTSGIEGAWTTHPMKWNTGKGGYFDLLLRYD
                     WWLQKSPAGAHQWEPVNIKEEDMPVDVEDPSIRHKPIMTDADMAMKFDPEYRKIAERFR
                     DDPAYFEKVFARAWFKLTHRDMGPKSRYIGPDVPAEDLIWQDPVPAGRKDYDVATVKAK
                     IVAAGLTIGEMVSTAWDSARTFRGSDKRGGANGARIRFAPQKDWEGNEPGRLAKVLAVY
                     EKIAVETGASVADVIVLAGNIGVEQAAKAAGVDVTVPFAPGRGDATPEQTDVESFEVLE
                     PLADGFRNWLKKDYVVSAEELLLDRTQLMRLTGCEMTALVGGMRVLGTNFGGTAHGVFT
                     DRVGALTNDFFVNLTDMAYSWKPTGRNSYAIVDRKTGATKWTATRVDLVFGSNSVLRAY
                     AEVYAQDDNKAKFVQDFVAAWTKVMNADRFDLH"
                     /name="katG"
     gene            complement(2349..2843)
                     /locus_tag="WP8W18C04_P30020"
     CDS             complement(2349..2843)
                     /locus_tag="WP8W18C04_P30020"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /inference="similar to AA sequence:RefSeq:WP_004200519.1"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="BBT47427.1"
                     /translation="MKEKKVKSMPMNTGISEADRKKIVEGLSALLADSYTLYLMTHNFH
                     WNVTGPQFNSLHMMFMDQYTEQWNALDVIAERIRALGFPAPGTYKEFVKLASIQEVEGV
                     PKANEMIAHLVAAQEATARTARKLFPVVDQANDQPTADVLTQRIDVHEKTAWMLRSLLE
                     D"
                     /name="DNA starvation/stationary phase protection protein"
     gene            complement(2894..3508)
                     /locus_tag="WP8W18C04_P30030"
     CDS             complement(2894..3508)
                     /locus_tag="WP8W18C04_P30030"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /inference="similar to AA sequence:RefSeq:WP_000904941.1"
                     /codon_start=1
                     /transl_table=11
                     /product="TniR protein"
                     /protein_id="BBT47428.1"
                     /translation="MLIGYVRVSKADGSQTTDMQRDALLAAGVDSAQIYEDHASGKRED
                     RPGLANCLKALRPGDTLIIWKLDRLGRDLRHLVNTVHDLTGRNIGLKVLTGHGAAIDTT
                     TAAGKLVFGIFAALAEFERELIAERTVAGLASARARGRKGGRPFKMTPAKLRLAMAAMG
                     QPETKVGDLCVELGIARQTLYRCISPQGELRPDGIRLLNQD"
                     /name="TniR protein"
     gene            3656..6622
                     /locus_tag="WP8W18C04_P30040"
     CDS             3656..6622
                     /locus_tag="WP8W18C04_P30040"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /inference="similar to AA sequence:RefSeq:WP_001138064.1"
                     /codon_start=1
                     /transl_table=11
                     /product="DDE transposase"
                     /protein_id="BBT47429.1"
                     /translation="MPRRSILSAAERESLLALPDTKDDLIRHYTFSESDLSIIRQRRGP
                     ANRLGFAVQLCYLRFPGVILGVDEPPFPPLLKLVANQLKVSIESWGEYGQREQTRREHL
                     VELQTVFGFQPFTMSHYRQAVHTLTELATQTDKGIVLANALIEHLRRQSVILPAPNAIE
                     QASAEAITRANRRIYDALAEPLSDAHRSRLDDLLKRRDNGKTTWLAWLRQSPVKPNSRH
                     MLEHIERLKAWQALDLPSGIERLVHQNRLLKIAREGGQMTPADLAKFEPQRRYATLVAL
                     AIEGMATVTDEIIDLHDRILGKLFNAAKNKHQQQFQASGKAINAKVRLFGRIGQALIEA
                     KQSGRDPFAAIEAVMCWDAFAESVTEAQKLAQPEDFDFLHRIGESYATLRRYAPEFLEV
                     LKLRAAPAAKNVLDAIEVLRGMNSDKARKVPADAPTEFIKPRWQKLVMTDTGIDRRYYE
                     LCALSEMKNALRSGDIWVQGSRQFKDFEDYLVPPAKFASLKLASELPLAVATDCDQYLH
                     ERLTLLETQLATVNRMALANELPDAIITESGLRITPLDAAVPDTAQALIDQMAMILPHV
                     KITELLLEVDEWTGFTRHFAHLKSGDLAKDKNLLMTTILADAINLGLTKMAESCPGTTY
                     AKLAWLQAWHIRDETYGAALAELVNAQFRHPFAEHWGDGTTSSSDGQNFRTGSKAESTG
                     HINPKYGSSPGRTFYTHISDQYAPFHTKVVNVGVRDSTYVLDGLLYHESDLRIEEHYTD
                     TAGFTDHVFALMHLLGFRFAPRIRDLGDTKLYIPKGDAAYDGLKPMIGGTLNIKHVRAH
                     WDEILRLATSIKQGTVTASLMLRKLGSYPRQNGLAVALRELGRIERTLFILDWLQSVEL
                     RRRVHAGLNKGEARNALARAVFFNRLGEIRDRSFEQQRYRASGLNLVTAAVVLWNTVYL
                     ERAANALRGHAQAVDDGLLQYLSPLGWEHINLTGDYLWRSSAKIGGGKFRPLRPLQPA"
                     /name="DDE transposase"
     gene            6701..7705
                     /locus_tag="WP8W18C04_P30050"
     CDS             6701..7705
                     /locus_tag="WP8W18C04_P30050"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /inference="similar to AA sequence:RefSeq:WP_000427614.1"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="BBT47430.1"
                     /translation="MENIALIGIDLGKNSFHIHCQDHRGKAVYRKKFTRPKLIEFLATC
                     PATTIAMEACGGSHFMARKLAELGHFPKLISPQFVRPFVKSNKNDFVDAEAICEAASRP
                     SMRFVQPRTESQQAMRALHRVRESLVQDKVKTTNQMHAFLLEFGISVPRGAAVISRLST
                     LLEDSSLPLYLSQLLLKLQQHYHYLVEQIKDLESQLKRKLDEDEVGQRLLSIPCVGTLT
                     ASTISTEIGDGKQYASSRDFAAATGLVPRQYSTGGRTTLLGISKRGNKKIRTLLVQCAR
                     VFIQKLEHQSGKLADWVRELLCRKSNFVVTCALANKLARIAWALTARQQTYEA"
                     /name="IS110 family transposase"
     gene            9202..9600
                     /locus_tag="WP8W18C04_P30060"
     CDS             9202..9600
                     /locus_tag="WP8W18C04_P30060"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /inference="similar to AA sequence:RefSeq:WP_003688254.1"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BBT47431.1"
                     /translation="MNSDQSYWLVGASWDGVDHQDKKFVDNKIWVLGYKEKDDESQYLK
                     AKKIKKGDRIAIKRMMGKGSANIKILHTGIVEGVVDYGSLVICVVNWLVLEKNIEVDSK
                     GAFASIHGPYKQGKDKDTDAWLNKVFSL"
                     /name="hypothetical protein"
     gene            9634..9882
                     /locus_tag="WP8W18C04_P30070"
     CDS             9634..9882
                     /locus_tag="WP8W18C04_P30070"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BBT47432.1"
                     /translation="MMKIDRLVRVTKKENSNGSSSTELEWEPGSKKEILKGAAAGAALG
                     SIVPVIGVASENGIYGHSRFCNTDFDDKLACLNLSGV"
                     /name="hypothetical protein"
     gene            complement(10064..11068)
                     /locus_tag="WP8W18C04_P30080"
     CDS             complement(10064..11068)
                     /locus_tag="WP8W18C04_P30080"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /inference="similar to AA sequence:RefSeq:WP_000427614.1"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="BBT47433.1"
                     /translation="MENIALIGIDLGKNSFHIHCQDHRGKAVYRKKFTRPKLIEFLATC
                     PATTIAMEACGGSHFMARKLAELGHFPKLISPQFVRPFVKSNKNDFVDAEAICEAASRP
                     SMRFVQPRTESQQAMRALHRVRESLVQDKVKTTNQMHAFLLEFGISVPRGAAVISRLST
                     LLEDSSLPLYLSQLLLKLQQHYHYLVEQIKDLESQLKRKLDEDEVGQRLLSIPCVGTLT
                     ASTISTEIGDGKQYASSRDFAAATGLVPRQYSTGGRTTLLGISKRGNKKIRTLLVQCAR
                     VFIQKLEHQSGKLADWVRELLCRKSNFVVTCALANKLARIAWALTARQQTYEA"
                     /name="IS110 family transposase"
ORIGIN
        1 ttcgctgctc caagtaccac ggatgcaaaa agagagaccg agaaacgatt gcttttcggt
       61 ctctccgaag gatgttcagc aggctgtcct gccatccaac ctagtgtaga tcaaagcgat
      121 ccgcattcat gactttcgtc catgcagcca caaagtcctg cacgaacttg gccttgttgt
      181 catcctgcgc gtagacctcc gcataggcac gcaacactga atttgaaccg aacaccagat
      241 caacgcgggt cgctgtccat tttgttgccc ccgtcttgcg gtcaacgatg gcgtagctgt
      301 tgcgccccgt cggtttccag ctgtaggcca tatcggtcag gttaacgaag aaatcattcg
      361 tcagcgcgcc aacacgatct gtgaacacgc catgcgcagt accgccgaag ttggtgccca
      421 gcacccgcat gcctcctacc aaggcggtca tttcgcaccc tgtcaaccgc atcaattggg
      481 tacgatcaag cagcagttcc tcagcactga ccacataatc cttcttcagc cagttgcgga
      541 aaccgtcggc caagggctca agcacctcga aggactcaac atcggtctgc tctggcgtgg
      601 catccccgcg tcctggcgcg aatggaaccg tcacgtcaac gccggcagcc ttagctgctt
      661 gttcgacgcc gatgttaccg gcaagcacga tcacatcagc gacactagca ccggtttcga
      721 cggcgatctt ttcatagact gccagcacct tggcaagacg ccccggttcg ttgccttccc
      781 agtccttttg cggggcgaag cgaatacgcg cgccgttcgc tccgccgcgc ttgtccgagc
      841 cacggaaagt gcgagcgctg tcccaagcgg ttgagaccat ctcaccgatt gtgagaccgg
      901 cggcgacaat ctttgccttg acggtggcaa cgtcgtagtc cttgcggccg gctggcaccg
      961 ggtcttgcca gatgaggtct tcggccggca cgtccgggcc gatgtaacgc gacttcggcc
     1021 ccatgtcacg gtgggtcagc ttgaaccagg cacgcgcgaa gactttctca aaatatgccg
     1081 gatcgtcgcg gaagcgctcg gcaatcttgc gatattcagg atcgaacttc atcgccatgt
     1141 ccgcgtcggt catgatcggc ttatggcgga tcgacgggtc ttcgacatcg accggcatat
     1201 cttcttcttt gatgttgact ggttcccact ggtgcgcgcc ggccggcgac ttctgtagcc
     1261 accagtcgta gcgaagcagc aaatcgaagt agccaccttt gccggtgttc catttcatcg
     1321 gatgcgtggt ccaagcgcct tcgataccgc tcgtcaccgt atcgcggccg atcccgcgag
     1381 tggtatggtt ctgccagccg agaccctgtt cttcgagttc ggctccttcg ggtgccgggc
     1441 cgaggttggc tgcactccca tttccgtgtg tcttgccaac cgtatggcca ccgcccgtta
     1501 gcgccacggt ttcctcatca tccattgcca tgcgcgcgaa ggtaatacgc acgtcgtgtg
     1561 ccgtcttcag cgggtcgggc ttgccgtcga caccttccgg gttcacatat atcaaaccca
     1621 tcatcacggc ggccagcggg ttttcgaggt cgcgctcgcc ggagtagcgg ctgccagcgc
     1681 tcccactggg ggcaagccat tctttttcag aaccccaata ggtgtctttc tcggggtgcc
     1741 agatgtcttc gcgaccgaag gcgaagccaa aggttttcag gcccatcgac tcgtaggcca
     1801 tggtaccggc gaggatgatc agatcggccc aggagagctt gttgccgtat tttttcttga
     1861 tcggccagag gagacggcgc gccttgtcga ggttggcatt gtctggccag gagttaagtg
     1921 gtgcgaagcg ctgattgccg gtaccaccac cgccacggcc gtcggcgatg cggtaggtgc
     1981 cggccgagtg ccaagccatg cggatcatca acccgccgta gtgaccccaa tcggccggcc
     2041 accaggactg gctatcggtc atcagtgctt tgaggtcatt cttcagcgct tgaacatcga
     2101 gcttcttgag ttcttcacga tagttgaagt ccgtacccag cgggttcgtc ttgctatcgt
     2161 gctgatgcag gatatccagg ttcagggcct tgggccacca ttccatggtc tgcatgctgg
     2221 ccgatgtagc gcctccgtgc atgaccggac attttccaga agaacttgcg tctttgtttt
     2281 ccatgtctca ttcctttgtg taatgaacag aaattgttag cccaatctag gccgactggt
     2341 gatctgcgtc agtcttcgag caagctacgc agcatccaag ccgttttttc atggacatcg
     2401 atacgctggg tcagcacgtc cgccgttggt tggtcgttgg cttgatccac caccgggaaa
     2461 agtttgcggg cagttctggc ggtagcttct tgcgctgcaa ccagatgggc gatcatctca
     2521 ttggccttgg gtactccctc gacctcctgg attgaagcga gcttgacgaa ttccttgtag
     2581 gtgccaggag ccgggaaacc caaggcacgg attcgctcgg cgatgacgtc caacgcattc
     2641 cactgttcgg tgtactgatc catgaacatc atgtgcaggc tgttgaactg cggcccggtc
     2701 acattccaat ggaagttgtg cgtcatcaag tacagggtgt agctgtcggc taacagagcg
     2761 gataacccct cgacaatctt cttgcggtca gcctctgaaa ttccagtatt catgggcatt
     2821 gatttaactt tcttttcttt catggtgctt tcctttcgtt gagataaatt gaatggacat
     2881 gttaacgcgg tcatcaatcc tgattgagaa gcctgattcc atcgggacgt agctcgcctt
     2941 gtggtgaaat acatcgatac aaggtctgac gtgcaatacc gagttcgaca cacaaatcgc
     3001 caaccttggt ttctggctgt cccattgccg ccattgccaa gcgcaacttg gcgggcgtca
     3061 tcttgaacgg ccgaccaccc tttcgaccgc gggcccgtgc tgaagccagg cccgccacgg
     3121 tgcgttcagc gattagctct cgctcgaatt ctgccagcgc agcgaagatg ccaaagacca
     3181 acttcccggc ggcggtcgtc gtatcgatgg ccgcgccgtg gcccgttaac accttgaggc
     3241 cgatgttgcg cccggtcaga tcatgcactg tgttgacgag atggcgcaga tcgcgtccaa
     3301 gacggtcgag tttccagatg attagcgtgt cacctggccg tagcgccttc aggcaatttg
     3361 ccaagcctgg gcggtcctct cgcttgccgg atgcgtggtc ttcgtagatc tgtgcggaat
     3421 caacgcccgc agctagcagc gcatcgcgtt gcatatcggt ggtctgagaa ccatccgctt
     3481 tcgatactcg cacgtagcca atcagcatgt tgagcctgtc acatatacgt tcgattatgt
     3541 gacactgtga ttttggcacg cgacggtgtc aatatatgtc acttaatccg tcaccttttc
     3601 taaggtgtgt aaacggtcat tgtagtaaaa aatgtgacaa ataaaaggaa ttcgcatgcc
     3661 ccgccgttcg attctctctg ccgccgagcg cgaaagcctg ctggcgttac cggacactaa
     3721 agacgatctg atccgacatt acacgttcag cgaaagcgac ctgtccatca tccggcaacg
     3781 gcgcggccca gctaaccggc tgggctttgc cgtacagctt tgctacctgc gctttcctgg
     3841 cgtcatcctg ggcgtcgatg aaccgccatt tccgcccttg ctgaaattgg tcgccaacca
     3901 gctcaaggtc agcatcgaaa gctggggcga gtacggacag cgggagcaga cccggcgcga
     3961 gcacctggtc gaactgcaaa cggtgtttgg cttccagccg ttcaccatga gccactaccg
     4021 gcaggccgtc cacacgctga ctgagctggc cacgcaaacc gacaagggca ttgtgctggc
     4081 caacgccttg atcgagcatc tgcggcggca gtcagtcatt ttgcctgccc cgaacgccat
     4141 cgagcaggcg agcgctgaag cgatcacccg cgccaatcgg cgcatctacg acgcattagc
     4201 cgaaccgctg tcggacgctc atcgcagtcg cctcgatgac ctgcttaagc gccgggacaa
     4261 cggcaaaacg acctggctgg cctggttgcg ccagtcaccg gtcaagccca attcgcggca
     4321 catgctggag cacatcgaac gcctcaaggc gtggcaggca cttgacctgc cttctggcat
     4381 cgagcggttg gttcaccaga accggctact caagatcgcc cgtgagggcg gtcagatgac
     4441 gcccgccgac ttggctaagt tcgagccgca gcgccgctac gcgaccttgg tagctctcgc
     4501 catcgagggc atggcgaccg tcaccgacga aatcattgac ctgcacgacc gtatcttggg
     4561 caagctgttc aacgccgcca agaacaagca tcagcagcag ttccaggcgt ccggcaaggc
     4621 gatcaacgca aaggtgcgat tgttcggtcg cattggtcag gcgctgatcg aggccaagca
     4681 gtcgggccgc gatccgttcg ccgccatcga ggccgtcatg tgctgggatg ctttcgccga
     4741 gagcgtcacc gaagcccaga agctcgcgca acccgaggac ttcgatttcc ttcaccgcat
     4801 cggcgagagc tacgccacgc tgcgacgcta cgcgccggaa ttccttgaag tgctcaagct
     4861 gcgggctgcg cccgccgcca agaacgtgct cgatgccatc gaggtgctgc gcggcatgaa
     4921 cagcgacaaa gcccgtaagg tgcctgcgga cgcgccgacc gaattcatca agccgcgctg
     4981 gcagaaactg gtgatgaccg acaccggcat cgaccggcgt tactacgaac tgtgcgcgct
     5041 gtcggagatg aagaacgcgc tgcgctcggg cgacatctgg gtgcaaggct cccgccagtt
     5101 caaggatttc gaggactact tggtgccgcc tgcgaaattc gccagcctca agctggccag
     5161 cgaattgccg ctagccgtgg ccaccgactg cgaccagtac ctgcatgagc gactgacgct
     5221 actggaaaca cagctcgcca ccgtcaaccg catggcgctg gcgaatgaat tgccggacgc
     5281 gatcatcacc gagtcgggcc tgaggatcac gccgctcgat gcggccgtgc ccgataccgc
     5341 gcaggccctg atcgaccaga tggcgatgat cctgccgcac gtcaagatca ccgaactgct
     5401 gctagaggtg gacgagtgga cgggcttcac ccggcacttc gcgcacctga aatcgggcga
     5461 tctggccaag gacaagaacc tgctgatgac cacgatcctg gccgacgcaa tcaaccttgg
     5521 cttgaccaag atggccgagt cctgccccgg cacgacctac gccaaactgg cttggcttca
     5581 agcctggcac atccgcgatg aaacctacgg ggcggcgctg gccgaactgg tcaacgcgca
     5641 attccgccat cccttcgccg agcattgggg cgatggcacc acgtcatcgt cagacggcca
     5701 gaacttccgc acaggcagca aggccgagag cacaggccat atcaatccga aatacggcag
     5761 cagtccggga cggacgttct acacccacat ctccgaccag tacgcgccgt tccacaccaa
     5821 ggtcgtgaat gtcggcgtgc gcgattcaac ctacgtgctc gacggcctgc tgtatcacga
     5881 atccgacctg cgcatcgagg aacactacac cgatacggca ggcttcaccg atcacgtctt
     5941 cgcactgatg cacctcttgg gcttccggtt cgcgccgcgc atccgcgacc tgggcgacac
     6001 caagctctat atcccgaagg gtgatgcagc ctacgacggg ctgaaaccga tgatcggcgg
     6061 cacgctcaac atcaagcacg tccgcgccca ttgggacgaa attttgcgtc tagccacctc
     6121 gatcaagcag ggcacggtga cggcctcgct gatgctcaga aagctcggca gctacccgcg
     6181 ccagaacggc ctggctgtgg cattgcgcga gctgggccgc attgagcgca cactgttcat
     6241 tttggactgg ctgcaaagcg tcgagctgcg ccgccgtgtg catgccgggt tgaacaaggg
     6301 cgaagcccgc aacgcactgg cccgggccgt gttcttcaac cgcttgggcg aaatccgcga
     6361 ccgcagtttc gagcagcagc gctatcgggc cagcggcctc aacctggtga cggcggccgt
     6421 tgtgctgtgg aacacggtct atctggagcg ggccgcgaac gccttacgtg gccacgctca
     6481 agccgtcgat gacggcctgt tgcagtacct gtcgccgctc ggctgggagc acatcaacct
     6541 gaccggcgat tacctctggc gcagcagcgc caagatcggc ggtggtaagt tcaggccgct
     6601 acgacccttg caacctgctt agcgtgcttt atttaatgag atggtcactc cctccttccc
     6661 agtactatgc tgaggacagg ctttcattcg gagaaccatc atggaaaaca ttgcgcttat
     6721 tggtatcgat ctgggtaaga actctttcca tattcattgt caggatcatc gtgggaaggc
     6781 cgtttaccgt aaaaaattca cccgaccaaa gctaatcgaa tttctggcga catgcccggc
     6841 aacaaccatc gcgatggaag cctgtggcgg ttctcacttt atggcacgca agctggcaga
     6901 gttagggcat tttccaaagc tgatatcacc gcaatttgtc cgcccattcg ttaaaagcaa
     6961 caaaaatgac ttcgttgatg ctgaagctat ctgtgaagca gcatcacgtc catctatgcg
     7021 tttcgtgcag cccagaaccg aatctcagca ggcaatgcga gctctgcatc gtgtccgtga
     7081 atccctggtt caggataagg tgaaaacaac taatcagatg catgcttttc tgctggaatt
     7141 tggtatcagc gttccgcgag gtgctgccgt tattagtcga ctgagtaccc ttcttgagga
     7201 cagtagtttg cctctttatc tcagccagtt actgctgaaa ttacaacagc attatcacta
     7261 tcttgttgag cagattaaag atctggaatc tcagttgaaa cgaaagttgg acgaagatga
     7321 ggttggacag cgcttgctga gtattccctg cgttggaacg ctgactgcca gtactatttc
     7381 aactgagatt ggcgacggga agcagtacgc cagcagccgt gactttgcgg cggcaacagg
     7441 gctggtaccc cgacagtaca gcacgggagg tcggacgaca ttgttaggga ttagcaagcg
     7501 gggcaacaaa aagatccgaa ctttgttggt tcagtgtgcc agggtattca tacaaaaact
     7561 ggaacaccag tctggcaagt tggccgactg ggtcagggag ttgttgtgtc ggaaaagcaa
     7621 ctttgtcgtc acctgtgctc tggcaaacaa gctggccaga atagcctggg cactgacggc
     7681 gcgacagcaa acttacgaag cataaaggca gaaatacacc agtttaaaca atcattcatc
     7741 tggttttgcg aatactgata ttgatgatac taacggccca ccggcctgtt gaggaacctg
     7801 taaaacggaa aggctcattg aagccgtata ttttctggag gttcatcagg cgcggaactc
     7861 atcgaggcgc gggaataaaa tcccattcag acgccggata gattcaagca agccaacttg
     7921 tcgtcaaaat cggtgttgca aaaacgggag tgaccataga ttccgttttc tgagacgacc
     7981 ccttattgga accggaattg gcgcagctat tggtgggact ctaggactga tttttgggaa
     8041 atctgaagaa aaaaaagata gtaaatgatt taccataaca cctcattacg cgcaccgctc
     8101 agacggcctg tgcgcgattt tccctcctga gcctcaaaac cacacacctt acccactgaa
     8161 aaccgctcag aatggcgcag atgatttctg aggccgtgaa tcgttatgca gccggctgcc
     8221 tggcgtccgg tttcagtgcg atcccatact taactgccaa aaaaacaaaa aatatgactt
     8281 tcacgaaaac ggtgaacggg caggcaattt attgccgaat cgtgaagcgt tggcggggta
     8341 tcggggcgca gccctgacca ggggatcaaa ggggatgaaa tccctttgca aggacgccga
     8401 taagagacag agcgcgaagc gttttgtata tacatcgggt agccttgttg cctttgaatt
     8461 tacgcaccgt ttctcccatt tatggagaaa cgtctaagtg cgcgttgctt ttatttttgg
     8521 ttttataaat tcagatctca aagccagcta acttgcctgg tttaaaaggt gaaagatgta
     8581 gattcagagt gaccgcgaat catgtgatct caggtccaat gacgcctcct cgctcattcg
     8641 ctcactccgt tcggtcatga gtctgtggcg ggcgttgaga gtgttactga aagggatttg
     8701 ggatttacta tggcccagat gtacaaaagc cccgtgacat aatcacaggg cttaggtacg
     8761 cttattcgtg cagatacctg ctacagataa gacctagaca atcaaatctt agcagggctg
     8821 gctagccatg tcactaccag cttaatacca ttcaacgaca ttaaaatcat atctgcacct
     8881 tagacgctca ggtctacgcc ccggactccg gcaggataaa caggcaaatt ggtgggaacg
     8941 taacttaacc gcgaaagagt taggctctac cccgtctcgc tgtacgataa caagcacctg
     9001 ataaaatgag cgaacaaggc agcagtaagc gggaaagtgt gattgtgaga agtctgacac
     9061 taaccccaca caaatcgaat cagttgctgg ataagacaca caagaaaaga gcttgacgaa
     9121 ggtcgctccg ctccccatgg tttttatata cccaccatgg gggcattagc tttctattct
     9181 tataaaaaac caggagacag aatgaatagc gatcaaagtt actggcttgt gggtgcaagc
     9241 tgggatggtg tagatcacca agacaaaaaa tttgtagata acaaaatctg ggttttgggc
     9301 tacaaagaaa aagatgatga aagtcagtat ttaaaggcaa aaaaaataaa aaaaggcgat
     9361 cgtatcgcta taaaaaggat gatgggaaaa ggctcagcca acataaaaat actccacaca
     9421 ggaatagtgg aaggagtagt cgactatggc tcattggtca tttgcgtagt caattggcta
     9481 gtactagaaa agaacattga agttgacagt aaaggtgcct ttgccagcat tcacggtcca
     9541 tacaagcaag ggaaggacaa ggataccgat gcatggctga acaaagtatt ctcactgtaa
     9601 ttagccgaca atcgaaaaca aaagggagaa cagatgatga aaatagatcg tcttgtaagg
     9661 gtcacaaaaa aagagaatag caacggttca tcgtcaaccg agttggaatg ggagcctggt
     9721 tcgaaaaaag agattctcaa aggagcagca gctggagcag cattagggag cattgtccct
     9781 gttattgggg tcgcttcaga aaacggaatc tatggtcact cccgtttttg caacaccgat
     9841 tttgacgaca agttggcttg cttgaatcta tccggcgtct gaatgggatt ttattcccgc
     9901 gcctcgatga gttccgcgcc tgatgaacct ccagaaaata tacggcttca atgagccttt
     9961 ccgttttaca ggttcctcaa caggccggtg ggccgttagt atcatcaata tcagtattcg
    10021 caaaaccaga tgaatgattg tttaaactgg tgtatttctg cctttatgct tcgtaagttt
    10081 gctgtcgcgc cgtcagtgcc caggctattc tggccagctt gtttgccaga gcacaggtga
    10141 cgacaaagtt gcttttccga cacaacaact ccctgaccca gtcggccaac ttgccagact
    10201 ggtgttccag tttttgtatg aataccctgg cacactgaac caacaaagtt cggatctttt
    10261 tgttgccccg cttgctaatc cctaacaatg tcgtccgacc tcccgtgctg tactgtcggg
    10321 gtaccagccc tgttgccgcc gcaaagtcac ggctgctggc gtactgcttc ccgtcgccaa
    10381 tctcagttga aatagtactg gcagtcagcg ttccaacgca gggaatactc agcaagcgct
    10441 gtccaacctc atcttcgtcc aactttcgtt tcaactgaga ttccagatct ttaatctgct
    10501 caacaagata gtgataatgc tgttgtaatt tcagcagtaa ctggctgaga taaagaggca
    10561 aactactgtc ctcaagaagg gtactcagtc gactaataac ggcagcacct cgcggaacgc
    10621 tgataccaaa ttccagcaga aaagcatgca tctgattagt tgttttcacc ttatcctgaa
    10681 ccagggattc acggacacga tgcagagctc gcattgcctg ctgagattcg gttctgggct
    10741 gcacgaaacg catagatgga cgtgatgctg cttcacagat agcttcagca tcaacgaagt
    10801 catttttgtt gcttttaacg aatgggcgga caaattgcgg tgatatcagc tttggaaaat
    10861 gccctaactc tgccagcttg cgtgccataa agtgagaacc gccacaggct tccatcgcga
    10921 tggttgttgc cgggcatgtc gccagaaatt cgattagctt tggtcgggtg aattttttac
    10981 ggtaaacggc cttcccacga tgatcctgac aatgaatatg gaaagagttc ttacccagat
    11041 cgataccaat aagcgcaatg ttttccatga tggttctccg aatgaaagcc tgtcctcagc
    11101 atagtactgg gaaggaggga gtgaccatct cattaaataa agcacgctaa ggaagttacg
    11161 agtttcagta catca
//
