LOCUS       AP022138                2421 bp    DNA     circular BCT 22-JUL-2020
DEFINITION  Enterobacter cloacae plasmid pWP5-S18-ESBL-01_5 DNA, complete
            genome, strain: WP5-S18-ESBL-01.
ACCESSION   AP022138
VERSION     AP022138.1
DBLINK      BioProject: PRJDB6962
            BioSample: SAMD00194528
            Sequence Read Archive: DRR199364
KEYWORDS    .
SOURCE      Enterobacter cloacae
  ORGANISM  Enterobacter cloacae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1
  AUTHORS   Sekizuka,T., Itokawa,K., Yatsu,K., Inamine,Y. and Kuroda,M.
  TITLE     complete genome sequences of Enterobacter cloacae str.
            WP5-S18-ESBL-01 isolated from wastewater treatment plant effluent
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2421)
  AUTHORS   Sekizuka,T., Itokawa,K., Yatsu,K., Inamine,Y. and Kuroda,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-DEC-2019) Contact:Tsuyoshi Sekizuka National Institute
            of Infectious Diseases, Pathogen Genomics Center; 1-23-1 Toyama,
            Shinjuku-ku, Tokyo 162-8640, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: flye v2.5; minimap v0.2-r124; racon v1.1.0;
            circlator v1.5.3; Pilon v1.18; SPAdes v3.11.1; A5-miseq v20140604
            Genome Coverage       :: 362x
            Sequencing Technology :: Sequel; NextSeq 500
            ##Genome-Assembly-Data-END##
            Annotated by DFAST v.1.2.3 (https://dfast.nig.ac.jp).
FEATURES             Location/Qualifiers
     source          1..2421
                     /organism="Enterobacter cloacae"
                     /mol_type="genomic DNA"
                     /strain="WP5-S18-ESBL-01"
                     /isolation_source="wastewater treatment plant effluent"
                     /db_xref="taxon:550"
                     /plasmid="pWP5-S18-ESBL-01_5"
                     /geo_loc_name="Japan:Tokyo"
                     /lat_lon="35.6538 N 139.5126 E"
                     /collection_date="2018-08-06"
     gene            complement(101..418)
                     /locus_tag="WP5S18E01_P50010"
     CDS             complement(101..418)
                     /locus_tag="WP5S18E01_P50010"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /inference="similar to AA sequence:RefSeq:WP_015705687.1"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="BBS39865.1"
                     /translation="MKKIFILSAALIMAGCATKQYPQAANVTGPESSAMDCKAVALEIA
                     KTRSVQQEIEATGEFDGRTVLGFLGDFGIGNGMAKGEARKKAANRLNQLEALKAAKCAD
                     Q"
                     /name="lipoprotein"
     gene            1834..2034
                     /locus_tag="WP5S18E01_P50020"
     CDS             1834..2034
                     /locus_tag="WP5S18E01_P50020"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BBS39866.1"
                     /translation="MNLCPDERLLFVRMISAMLRRSGGDAGAVMFEAYRHIVSDTNQAR
                     RSCMLDLLESVRHDYVHGGYT"
                     /name="hypothetical protein"
     gene            complement(2082..2324)
                     /locus_tag="WP5S18E01_P50030"
     CDS             complement(2082..2324)
                     /locus_tag="WP5S18E01_P50030"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BBS39867.1"
                     /translation="MNEVRFQSAITIKPGKSRMTKQESAALNMAKFIRAQSLLLLEKLD
                     VLDLDDEAADCERMHEQAEALYQKLSARFGIQDGE"
                     /name="hypothetical protein"
     Typing          complement(626..1146)
                     /name="rep_cluster_2358"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="88.868"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 aacgccctga gagcctctca ggcggtgcac gcaacagatg ttatggtaaa tccagctgat
       61 tggcaggtac aaaaaaagcc cggcagggct tttttgtacc ttattgatct gcacactttg
      121 cagccttcag ggcttccagc tgatttagcc ggttagctgc ttttttgcgg gcttcgcctt
      181 ttgccatgcc atttcctatc ccaaaatcac ccaggaaacc taatacggtg cgcccgtcaa
      241 attcgccggt tgcctcaatt tcttgttgta cactgcgcgt tttggcgatt tccagagcta
      301 ccgctttgca atccattgcg gaagattctg gaccggtgac gttcgcggcc tgtggatatt
      361 gctttgttgc gcatcctgcc atgatcagcg ctgccgatag gatgaatatt tttttcaaaa
      421 ttatccttat ttatttaggc atagttaaga gatacccatt ttatcattta ggcatagtta
      481 agagataccc attttatcgg caggaaatga gggtttttta gttgaatttt gggaggggca
      541 aggttccata ttggtcgagg attctggagc tgaccggcaa cggccagcgc ggctatgggc
      601 catgcggtgg ttgagcatag aaaggatctt cttgagttcc tttttttcta cgcgtaatct
      661 cttgctctga aaacgaaaaa accacctggg gaggtggttt ttcgaaggtt aagtcagttg
      721 gggaactgct taacctggta actggcttca gcagagcgca gataccaaat actgtccttc
      781 cagtgtagcc gtagttaggc caccacttca agaactctat agcaccacct aaattaactc
      841 gctctgctaa tcctgttacc agtggctgct gccagtggcg ctttgtcgtg tctttccggg
      901 ttggactcaa gacgatagtt accggataag gcgcagcagt cggactgaac ggggggttcg
      961 tgcatacagt ccagcttgga gcgaactgcc ttcccggaac tgagtgtcag gcgtggaatg
     1021 agataaacgc ggccataaca gcggaatgac accggtaaac cgaaaggcag gaacaggaga
     1081 gcgcacgagg gagccaccag ggggaaacgc ctggtatctt taagtcctgt cgggtttcgc
     1141 caccactgat ttgagcgtca gatttcgtga tgcttgtcag gggggcggag cctatggaaa
     1201 accggcaacg ccgcggcctt ttcttttgcc tctcgatcct ctctcattgc ctgtgcctta
     1261 atgtctccgt tccctccgct cgccgcagcc gaacgaccga gcgtagcgag tcagtgagcg
     1321 aggaagcgga atatccccgg acttatcttc ggcaccgtac gcgccagtgc tgcatttttt
     1381 aatacgatgc aggatagggc aaacgccgca aaatgacgtc atccgacatc attccgccgc
     1441 gttcgtccag tgacctacag tcggctctgg ggtgtcgggg ctgtgccctg accaggcgtt
     1501 ttggagtatc gccaccgtgg tggcggtgct acaaaacata tcctgtccct tacttggcat
     1561 ctctgaagtt tagccagtat acactccgct agcgctacgt gactggttca gggctgcgcc
     1621 ccgacacccg ctaaaagcac tgacgcgcct gcggcttgcc caaccccgcg ccgtccggga
     1681 aagaatttcc cgatcgaccc gacgtttctg tttacctgtg atgttaacgt ttggatcttt
     1741 ttcccgggtt tacctatttt cttctaatga agcgttcaca gctgacgtta atagatgggt
     1801 gctttcatgt ttggtttttc accaggagaa cttatgaacc tttgccccga tgaacgtctg
     1861 ctctttgtgc ggatgatttc cgctatgctt cgccgttcgg gcggtgatgc gggtgctgtt
     1921 atgtttgagg cgtatcgcca tattgtgtcc gataccaatc aagctcgtcg ttcctgcatg
     1981 ctcgacctgc tcgaaagcgt caggcatgac tatgtacacg gtggatatac atagttgccc
     2041 tggatacgct tttcgtctaa gtgagcgttc ttttcgtctc tttattcgcc gtcctgtata
     2101 ccgaagcgtg cgctcagctt ctgataaagc gcttctgcct gttcgtgcat tcgctcacag
     2161 tcggctgcct cgtcatcaag atcgagcacg tcgagttttt cgagcagcag cagcgactgc
     2221 gcccggataa attttgccat gttcagtgcg gctgattcct gttttgtcat acggcttttt
     2281 cccggtttta tggtgatcgc agactgaaat cttacttcat tcacttgagt gcatagccat
     2341 gcaggtacgc agaaatcatc agcgcgtttc tgagcggttt tcagggggta agatgtgttc
     2401 ttttgcaggt gaggtcgtag a
//
