LOCUS       AP020334                6237 bp    DNA     circular BCT 14-APR-2020
DEFINITION  Salmonella enterica SESen3709 plasmid pSESen3709_2 DNA, complete
            genome.
ACCESSION   AP020334
VERSION     AP020334.1
DBLINK      BioProject: PRJDB8208
            BioSample: SAMD00168437
            Sequence Read Archive: DRR174990, DRR174991
KEYWORDS    .
SOURCE      Salmonella enterica
  ORGANISM  Salmonella enterica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Salmonella.
REFERENCE   1
  AUTHORS   Shigemura,H., Sakatsume,E., Sekizuka,T., Yokoyama,H., Hamada,K.,
            Etoh,Y., Carle,Y., Mizumoto,S., Hirai,S., Matsui,M., Kimura,H.,
            Suzuki,M., Onozuka,D., Kuroda,M., Inoshima,Y. and Murakami,K.
  TITLE     Food workers as a reservoir of extended-spectrum
            cephalosporin-resistant Salmonella in Japan
  JOURNAL   Appl. Environ. Microbiol. (2020) In press
   PUBMED   32276982
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6237)
  AUTHORS   Shigemura,H., Sekizuka,T., Etoh,E., Carle,Y., Kuroda,M. and
            Murakami,K.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2019) Contact:Hiroaki Shigemura Fukuoka Institute
            of Health and Environmental Sciences; Mukaizano 39, Dazaifu
            818-0135, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: Canu v. 1.4; minimap v. 0.2-r124; racon v.1.1.0;
            circlator v. 1.5.3; Pilon v. 1.18; SPAdes v. 3.11.1; A5-miseq v.
            20140604
            Genome Coverage       :: 286x
            Sequencing Technology :: Sequel; MiSeq
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6237
                     /organism="Salmonella enterica"
                     /mol_type="genomic DNA"
                     /strain="SESen3709"
                     /serovar="Senftenberg"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:28901"
                     /plasmid="pSESen3709_2"
                     /geo_loc_name="Japan"
                     /collection_date="2017"
     gene            101..445
                     /gene="mobC"
                     /locus_tag="SESEF3709_04780"
     CDS             101..445
                     /gene="mobC"
                     /locus_tag="SESEF3709_04780"
                     /inference="ab initio prediction:Prodigal:2.6"
                     /inference="similar to AA sequence:UniProtKB:P13657"
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein MobC"
                     /protein_id="BBN79848.1"
                     /translation="MADKRSKMLTMWVTEDEHRRLLERCDGKQLAAWMRQTCLDEKPAR
                     AGKLPSISPALLRQLAGMGNNLNQIARQVNAGGGSGHDRVQVVAALMAIDAGLERLRHA
                     VLEKGADDDR"
                     /name="mobC"
     gene            435..1928
                     /gene="mbeA"
                     /locus_tag="SESEF3709_04781"
     CDS             435..1928
                     /gene="mbeA"
                     /locus_tag="SESEF3709_04781"
                     /inference="ab initio prediction:Prodigal:2.6"
                     /inference="similar to AA sequence:UniProtKB:P13658"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA relaxase MbeA"
                     /protein_id="BBN79849.1"
                     /translation="MIVKFHPRGRGGGAGPVDYLLGKDRQREGATVLQGKPEEVRELID
                     ASPYAKKYTSGVLSFAEKDLPPGQREKLMASFERVLMPGLDKDQYSVLWVEHQDKGRLE
                     LNFLIPNTELLTGKRLQPYYDRADRPRIDAWQTIVNGRLGLHDPNAPENRRALVTPSAL
                     PETKQEAAQAITRGLLALASSGELKTRQDVTEALESAGFEVVRTTKSSISIADPDGGRN
                     IRLKGAIYEQSFNAGEGLRAEIESAAAEYRRDAESRIQRAREVCQSGTERKREENQRRH
                     PRPRPEAVLSHEPAYERDAAHGQPDVADHRPGLRAADRLERGHSVVAESADTRQLREHP
                     GAEEHAGDAVRAEQRRAALDLRRAETPLREGEPGSGTVRRGFELDDTGGEIAHDRAGKT
                     VAERIRAATAGLLEKAGRVGERLRGMADDVWSYATGERGAERARHGLEQAGAEFERSAA
                     PVVERLNAVELQQVREARHQKALELERSQRQRVYHGPSL"
                     /name="mbeA"
     gene            2098..3012
                     /locus_tag="SESEF3709_04782"
     CDS             2098..3012
                     /locus_tag="SESEF3709_04782"
                     /inference="ab initio prediction:Prodigal:2.6"
                     /inference="protein motif:Pfam:PF05118.9"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl/Asparaginyl beta-hydroxylase"
                     /protein_id="BBN79850.1"
                     /translation="MLSLIIIFVFLISVLYAHSRGKIKQKFSRQLFDHSTFMAPINMFM
                     TGFSKLPSKQAWFPPETFPELQPLIDNWQVIRDEAVNLQGHIKSSEKNNDAGFNTFFKR
                     GWKRFYLKWYGDAHPSAERLCPVTTQLVSSIPCIKAAMFAELPSGSHLGKHRDPYAGSV
                     RYHLGLSTPNDERCFIEVDGVRRSWHDGEVIIFDETFVHWAQNDTEQTRIILFCDIERP
                     MKWRWSQKINRWIGGVLMSAAASPNDNNDRTGAINHLFKYVNAIRDAGQKLKKRNRKLY
                     YLIKNGVISLIFVWLAVSVFRWL"
                     /name="aspartyl/Asparaginyl beta-hydroxylase"
     gene            3675..4211
                     /locus_tag="SESEF3709_04783"
     CDS             3675..4211
                     /locus_tag="SESEF3709_04783"
                     /inference="ab initio prediction:Prodigal:2.6"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BBN79851.1"
                     /translation="MLDTDCITVLTARGITEILNSGGSQAWRLDATHVAKLPYLVCVQN
                     SKKDWGSQEAKHHHAFMIGKISGVSRAPENPKRWIINIDSYAEIDIPGQWDGNRNPVSY
                     RKLEDMGIDISKVDFQPLPKEDSLVLNTIKGGDEYDEYEEDSNRVRPINLKEAKAGLAL
                     YFGVNEDDIQITIKG"
                     /name="hypothetical protein"
     gene            4216..4806
                     /gene="hcpC_4"
                     /locus_tag="SESEF3709_04784"
     CDS             4216..4806
                     /gene="hcpC_4"
                     /locus_tag="SESEF3709_04784"
                     /inference="ab initio prediction:Prodigal:2.6"
                     /inference="similar to AA sequence:UniProtKB:O25728"
                     /codon_start=1
                     /transl_table=11
                     /product="putative beta-lactamase HcpC precursor"
                     /protein_id="BBN79852.1"
                     /translation="MPSLSVERWLALATAGDSQARFHLGKIFSCGLGVHQDYKQAIYWF
                     QLAAEQGHDDAQFSLGWAYHLGLGGYKDAQKASEWYQKAAEQGHAEAQFQLGRLYAFSD
                     LRNDALAVEWFLCAAKQDHSSAQFKLGWMYHLGMGVEQSDRQAFHWFILAAEQDHAGAQ
                     ASLGEMYKNGWGVEQDDVAAQDWFYRARTMHYP"
                     /name="hcpC_4"
     gene            4943..5350
                     /locus_tag="SESEF3709_04785"
     CDS             4943..5350
                     /locus_tag="SESEF3709_04785"
                     /inference="ab initio prediction:Prodigal:2.6"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BBN79853.1"
                     /translation="MGSDVQWQIDNGYLAQVMGDFSTESPSFETPMFGRYFDTFQAAIH
                     VAKAFPGSTLQPSEYEDKTGMLIRHYYVDCHPYYDRLPPEKSDNFQYDEYPSAIHVAAC
                     IDQASKELDELLFGGGYGENGEGHASPIILY"
                     /name="hypothetical protein"
     Typing          complement(5606..6089)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="88.09"
                     /coverage="92.0"
                     /evalue="3.59e-159"
     Typing          436..1925
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="91.182"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ccgaaacccg ctaaaaccac tgacgcgcct gcggcttgtc caacaccgcg ccgaccggga
       61 aaagatttcc cgcccgtccc ggcgttatca ggaggccgga ttggcagaca aacgcagcaa
      121 aatgctcacg atgtgggtga ccgaggatga gcaccggcgt ctgctggagc gctgcgacgg
      181 taaacagctt gcagcctgga tgcgccagac gtgcctggac gagaaacccg cccgcgccgg
      241 caagcttccc tcgatctcgc cggcgctgct tcgtcagctt gccggcatgg ggaacaacct
      301 taaccagatt gcccggcagg ttaacgccgg tggtggcagc gggcacgacc gcgtgcaggt
      361 cgtcgccgcg ctgatggcca tagatgccgg actcgagcgg ctgcggcatg ccgtgctgga
      421 aaagggtgca gacgatgatc gttaagtttc acccgcgagg gcgcggcggt ggcgccggtc
      481 cggtggatta tctgctgggg aaagaccgac agcgcgaggg cgccaccgtt ctgcagggga
      541 agccggaaga agtcagggag cttatcgacg cctcacccta cgcaaaaaag tacacatccg
      601 gcgtcctgtc ctttgccgaa aaggatttac cgcccgggca gcgcgaaaag ctgatggcga
      661 gctttgaacg ggttctgatg cccggactcg acaaagacca gtacagcgtg ctgtgggttg
      721 agcaccagga taaggggcgg ctggagctga acttcctgat cccgaacacg gagctgctga
      781 ctggcaagcg tctccagccg tattacgacc gcgccgaccg tccgcgcatt gatgcctggc
      841 agaccatcgt gaacggcagg ctggggctgc atgacccgaa cgcgcctgaa aaccggcggg
      901 cgctggtcac gccgtccgcg ctgccggaaa cgaagcagga agccgctcag gcaattacac
      961 ggggtttact ggccttggcc tcgtccgggg agctgaaaac ccgccaggac gtcactgagg
     1021 cgctggaaag cgcaggtttt gaggtggtac gcaccacaaa aagcagcatc agcattgccg
     1081 acccggacgg ggggcgaaac atccgactga agggagccat ctatgaacag tcttttaacg
     1141 ctggcgaagg acttagagca gaaatcgaaa gcgcagcagc agagtaccgg cgagatgctg
     1201 aaagccgcat tcagcgagca cgagaagtct gtcagagcgg aactgagcga aagcgagaag
     1261 agaatcagcg ccgccatcct cgaccacgac cggaagctgt cctcagccat gagccagcgt
     1321 acgaaaggga tgctgcgcat ggtcagccag acgtggctga ccatcgtcct ggtctccgtg
     1381 ctgctgatcg cctcgaacgc ggccattctg tggtggcaga gtcagcagat actcgacaat
     1441 tacgtgagca tccgggagca gaagagcacg caggcgatgc tgtccgagcg gaacagcggc
     1501 gtgcagctct cgacctgcgg cgagcagaga cgccgctgcg tgagggtgaa cccggaagcg
     1561 ggacggttcg gagaggattc gagctggatg atactggcgg ggaaatagca catgacagag
     1621 ctggaaaaac agttgctgag cgcattagag cagctacagc aggactactc gaaaaggctg
     1681 gacgagtggg agagcgcctt cgcggaatgg cggacgatgt ctggtcttat gcaacgggag
     1741 aacgcggcgc tgagcgagcg cgtcacgggc ttgagcaggc aggtgcagag tttgagcgat
     1801 cagctgcgcc ggttgtcgag cggctgaatg ccgtcgagct gcagcaggtg cgggaggcgc
     1861 ggcatcagaa agcgctggag ctcgaacgat cacagcgaca gcgggtatat cacggtccgt
     1921 ccctttgatt catttcagca taattcctgc tgaaatcctc tctgcctgtg tagtagatat
     1981 gttctggtga agcttcgatt atactattga taaatagcat ttagattata atttaagtgc
     2041 attcattatg ttatctttaa gagattttta ttattggcgt gaacaaggaa tatacacatg
     2101 ctgtcactta ttattatttt tgtttttctt atcagtgtat tgtatgcgca ttctcgcggt
     2161 aaaataaaac aaaagttttc caggcagtta tttgatcatt caacatttat ggctccgata
     2221 aatatgttta tgacaggctt ttcgaagctg ccgtcaaagc aggcatggtt tcccccagaa
     2281 acatttcctg aacttcaacc attaattgat aactggcagg ttataagaga cgaagctgtt
     2341 aatttgcaag ggcacattaa atcatctgaa aaaaataatg atgcaggatt taatacattt
     2401 tttaagcgcg gttggaagcg cttttacctc aagtggtatg gagatgctca tccatcagcg
     2461 gagcggcttt gcccagtaac aacccaactg gttagctcta ttccctgtat taaagcagcg
     2521 atgtttgctg aattgccttc aggaagccat ttaggaaagc atcgtgaccc ttatgccggt
     2581 tcagttcgtt atcatttagg attatcaaca ccaaatgatg aacggtgttt tattgaagtt
     2641 gatggtgtac gccgcagttg gcatgatggt gaagtgataa tttttgacga aacttttgtt
     2701 cactgggctc agaatgatac cgagcagaca cgtattattt tgttctgtga tatagaaagg
     2761 ccgatgaagt ggcggtggtc acaaaaaata aataggtgga taggtggggt gctaatgtca
     2821 gcggcagcat cacccaacga taataatgac aggactggtg ctataaatca tttatttaaa
     2881 tatgttaatg ctattcgtga tgcaggtcag aagctaaaaa aaaggaatcg aaaactatat
     2941 tatttgatta aaaatggagt tatttctttg atttttgttt ggcttgctgt ctcagtgttt
     3001 agatggctgt gatttgtttt tttctattga gtcatacata aatttgggtc ttctatgctc
     3061 aatgtatggt tatagataaa aatcgcctgc taaaaaattc tattgaaatc tttttagtaa
     3121 gaaaggcccg aatctgacgc tcagcgggcc tttcgtgaag aagtgcggca gtagacagat
     3181 ttcatctgat cacgttcatg tgctgctgtg caattaagcg tttctttcgg agctttcacg
     3241 gtatttcctt cacgaaaccg gccgccattc actcttgtgc atagccatgc agccgcgcag
     3301 aaattatcag gggcgttttt cagggtttgg gccggggaaa ggtgccggaa acggccctca
     3361 ggccgcagaa acgcgctgag gggctctcag ggggtgcggc caagaactct tatgttaaat
     3421 gcggcggtgt ggcgggttct tacttcacac atcatgttgt cactcagact gagtccagag
     3481 agaaaaagcc ctccggccga agtcgagtag tgttttactg gtttttttgc agccctagga
     3541 gtaatcgtcg tagtacttcg gatagttatt tgtatatatc ttgtggtttt atgttgagct
     3601 cttccgtttt tgatgctaaa aagattgtca ttacgcggtg acttgccttt tttaaaacaa
     3661 aaatggagat atctatgctt gatactgact gcattactgt tcttacggcc aggggtatta
     3721 ctgagatttt aaatagtgga ggaagccagg cttggagact tgatgctact cacgtagcta
     3781 agcttccgta cctggtttgc gtccaaaatt cgaaaaaaga ctggggtagt caggaggcta
     3841 agcatcatca tgcatttatg atcgggaaaa tatccggagt aagtcgagcc cctgaaaacc
     3901 ctaagcgctg gatcataaat attgattcct atgctgaaat tgatatccct gggcagtggg
     3961 acggcaatcg taatccagtc tcctatagaa aacttgaaga catgggaatt gatatctcca
     4021 aagtggattt ccaaccccta cctaaagagg attccttagt cttgaatacg ataaaaggcg
     4081 gggatgaata tgatgagtat gaggaggata gcaacagagt taggccgata aatctaaaag
     4141 aggctaaagc agggttggct ttatattttg gtgtgaatga ggatgatatt cagatcacaa
     4201 ttaagggatg accttatgcc gtcactctct gttgagcgct ggttagcact agctactgct
     4261 ggagatagcc aggctagatt tcatctgggt aagatattct catgcggtct tggtgtgcat
     4321 caggattata agcaggctat atactggttt cagcttgcag ctgagcaagg ccatgatgat
     4381 gcccagttta gtcttggttg ggcatatcat ctggggttag gtggatataa agatgctcaa
     4441 aaagcttcgg aatggtatca gaaagctgct gagcaaggtc atgcagaggc tcagtttcag
     4501 cttggaaggc tgtatgcttt ctctgattta agaaatgatg cgttagcagt cgaatggttt
     4561 ctctgtgctg cgaagcaaga ccacagtagt gcccaattta aacttggctg gatgtaccat
     4621 ctcggtatgg gcgttgagca aagtgatagg caggctttcc attggtttat tctggccgct
     4681 gagcaagatc atgcaggagc tcaggcatct cttggtgaga tgtataaaaa tggatggggg
     4741 gtcgagcaag atgatgttgc tgcgcaggat tggttttatc gtgccagaac catgcactat
     4801 ccctaaaccc gccctgtgcg ggttttttta cgcctacgtg taagaggctt gcggagctgg
     4861 ctgctgctga ttcataacat gaagcgttat ggtaaataca tattatggtt atttattagg
     4921 agtaactctg ggagagtggg ctatgggatc agatgttcaa tggcagatag ataatggtta
     4981 tttggcgcaa gttatggggg atttttcgac agagtcgcct agctttgaaa cgccaatgtt
     5041 tgggcgatat tttgatacct ttcaagctgc tatacatgtt gcaaaagctt tcccagggag
     5101 tacgttgcag ccatcagagt acgaagataa aactgggatg ctaatacgtc attattatgt
     5161 ggattgtcat ccgtattatg atcgactccc tcctgaaaaa agcgataatt ttcagtatga
     5221 tgagtaccct tctgctattc atgtggctgc gtgtattgat caagctagca aagaactcga
     5281 cgaactattg tttggtggtg gatatgggga aaatggtgag gggcatgcaa gcccgataat
     5341 tttatattag tgttaggggc ggagggatat tatccaattt gtaaaatgat cttcttgaga
     5401 tcgttttgct ctgcgcgtaa tctcttgctc tgaaaacgaa aaaaccacct ggggaggtgg
     5461 tttttcgaag gttcagtaag ttggggaact tctgaaccgt ggtaacagtc ttgtgcgaga
     5521 cgtgtcacca aatctgtcct tccagtgtag ccgtagtccg gccaccactt caagaactct
     5581 cgatacatct ctcgcacatc ctgcttacca gtggctaccg ccaatggcga ttttgtcgtg
     5641 tctttccggg ttggactcaa gttgatagtt accggaatgg gcgtggtagt cggactgaac
     5701 gggggggtcg tgcatacagt ccagcttgga gcgaactgcc tacccggaac tgagtgtcag
     5761 gcgtggaatg agacaaacgc ggccacataa cagcggaatg acaccggtaa accgaaaggc
     5821 aggaacagga gagcgcacga gggagctacc agggggaaac gcctgggatc tttatagtcc
     5881 tgtcgggttt cgccgccact gatttgagcg tcatatttcg tgatgcttgt caggggggcg
     5941 gagcctatgg aaaaacggca acgccgcatc cttttcccag cccttcctga tttgagcgcc
     6001 atctctttat cccgccattc gtgagccggt aacgcccgcc gcagtctcac ggcaggagcg
     6061 tagcgaccga gtgagcgagg aggcgtcatt tcatcgtccc cagcatttcg cacttacgcc
     6121 ccacagccgc attttttacc tgccacatga agcacttccc tgaactccca cttcgattta
     6181 ccatattaag ccagtataca ctccgctagc gctacgtgac tggttcaggg ctgcgcc
//
