LOCUS       AP020331                4593 bp    DNA     circular BCT 14-APR-2020
DEFINITION  Salmonella enterica SEHaa3795 plasmid pSEHaa3795_1 DNA, complete
            genome.
ACCESSION   AP020331
VERSION     AP020331.1
DBLINK      BioProject: PRJDB8208
            BioSample: SAMD00168438
            Sequence Read Archive: DRR174992, DRR174993
KEYWORDS    .
SOURCE      Salmonella enterica
  ORGANISM  Salmonella enterica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Salmonella.
REFERENCE   1
  AUTHORS   Shigemura,H., Sakatsume,E., Sekizuka,T., Yokoyama,H., Hamada,K.,
            Etoh,Y., Carle,Y., Mizumoto,S., Hirai,S., Matsui,M., Kimura,H.,
            Suzuki,M., Onozuka,D., Kuroda,M., Inoshima,Y. and Murakami,K.
  TITLE     Food workers as a reservoir of extended-spectrum
            cephalosporin-resistant Salmonella in Japan
  JOURNAL   Appl. Environ. Microbiol. (2020) In press
   PUBMED   32276982
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4593)
  AUTHORS   Shigemura,H., Sekizuka,T., Etoh,E., Carle,Y., Kuroda,M. and
            Murakami,K.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2019) Contact:Hiroaki Shigemura Fukuoka Institute
            of Health and Environmental Sciences; Mukaizano 39, Dazaifu
            818-0135, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: Canu v. 1.4; minimap v. 0.2-r124; racon v.1.1.0;
            circlator v. 1.5.3; Pilon v. 1.18; SPAdes v. 3.11.1; A5-miseq v.
            20140604
            Genome Coverage       :: 336x
            Sequencing Technology :: Sequel; MiSeq
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4593
                     /organism="Salmonella enterica"
                     /mol_type="genomic DNA"
                     /strain="SEHaa3795"
                     /serovar="Haardt"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:28901"
                     /plasmid="pSEHaa3795_1"
                     /geo_loc_name="Japan"
                     /collection_date="2017"
     gene            order(4366..4593,1..111)
                     /gene="mbeC_1"
                     /locus_tag="SEBLO3795_04573"
     CDS             join(4366..4593,1..111)
                     /gene="mbeC_1"
                     /locus_tag="SEBLO3795_04573"
                     /inference="ab initio prediction:Prodigal:2.6"
                     /inference="similar to AA sequence:UniProtKB:P13657"
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein MbeC"
                     /protein_id="BBN75185.1"
                     /translation="MKREKMLTIRVSDEEHARLLERCEGKRLAEWMRRVCLGEPVARTG
                     KLPTLSPPLLRHLAAIGNNLNQTARKVNSGHWSSIDRVHVVAALMAIEGELRQLRQAVR
                     EQGGRDDS"
                     /name="mbeC_1"
     gene            101..1690
                     /gene="mbeA"
                     /locus_tag="SEBLO3795_04574"
     CDS             101..1690
                     /gene="mbeA"
                     /locus_tag="SEBLO3795_04574"
                     /inference="ab initio prediction:Prodigal:2.6"
                     /inference="similar to AA sequence:UniProtKB:P13658"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA relaxase MbeA"
                     /protein_id="BBN75182.1"
                     /translation="MIVKFHARGKGGGSGPVDYLLGRERNREGATVLRGNPEEIRELID
                     ATPFSKKYTSGVLSFAEKELPPGERERVMTSFERVLMPGLEKDQYSILWVEHQDKGRLE
                     LNFVIPNMELQSGKRLQPYYDRADRPRINAWQTLVNHHYGLRDPNAPENRRTLVTPNNL
                     PKAKQEAAEAITRGLEALYRAGAIKTRQDVTEALTAAGFEVVRTTRSSISIADPEGGRN
                     LRLKGALYEQSFTNGNGLREETERASRFYREHAEQRVQEARRVCRQGTELKQAENQRRY
                     QRTYNIRETATGYAEHHREHAAQHHRGRTVPAAEKPALAGDQRAGTVSGADRAQRHGRH
                     FPGVLGADSVAGKQDCEKYLDHQRAGKHAGETRGKNLGGDVRQWREREISGAAGRGERG
                     KHLDGGRQERREAGQGVNEYDGTGRAFAERLRAFATGLYAAAERVGERLRRIAEDARGY
                     ATEQRDTERACRELESAGAAISRTSRPLESVICHEQQIRDEQERQAQELARRWHEEQEP
                     EARIWRGPSMGM"
                     /name="mbeA"
     gene            complement(2277..3116)
                     /locus_tag="SEBLO3795_04575"
     CDS             complement(2277..3116)
                     /locus_tag="SEBLO3795_04575"
                     /inference="ab initio prediction:Prodigal:2.6"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BBN75183.1"
                     /translation="MLTLFIFFVLLIAACFFCFAPPRRGYDRNEIIPYKIKLSINKYRL
                     YIYSSGKVRQYLLFLVILSLYYSIAEPFKSELIKNISYSLMAAFIFDTGLNFSKENITK
                     GVISTRWHNDLYSSFERMKAINKIYYPSNKEINTEGLSKAITSSLFNDDANSFAKRDFR
                     LMWDLSSEKYLSYKEIIIRKGDKLDAVCLRFINDDYKFLVNFNRDEEVFKYFPSIMQPS
                     LKTYRALSRLVNSIKDPSRFKFTTESLEMELLEYLELRNELFNDIEEVMGSYAQRAP"
                     /name="hypothetical protein"
     gene            complement(4127..4318)
                     /gene="rop"
                     /locus_tag="SEBLO3795_04576"
     CDS             complement(4127..4318)
                     /gene="rop"
                     /locus_tag="SEBLO3795_04576"
                     /inference="ab initio prediction:Prodigal:2.6"
                     /inference="similar to AA sequence:UniProtKB:P03051"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein rop"
                     /protein_id="BBN75184.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGESN"
                     /name="rop"
     Typing          3955..4039
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="3.03e-37"
     Typing          complement(3386..3944)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.761"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          102..1687
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.811"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          4349..4593
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="84.146"
                     /coverage="100.0"
                     /evalue="6.87e-40"
ORIGIN
        1 tggtcttcga ttgaccgggt tcatgtggtg gccgctctga tggccatcga gggtgaattg
       61 cgccagttgc ggcaggctgt cagggaacag gggggacggg atgatagtta aatttcatgc
      121 cagggggaaa ggcggtggaa gtggcccggt cgattacctg ctggggcggg agcgtaaccg
      181 cgaaggcgca acggtgctcc ggggtaaccc ggaagaaatc cgggaactca ttgatgccac
      241 gccattttcg aagaaataca cgtcaggcgt tctgtcgttc gcggagaagg agttaccgcc
      301 gggtgaacgc gaaagagtga tgacgagctt tgagcgtgtt cttatgcccg gtctcgaaaa
      361 agaccagtac agcattctct gggtggaaca ccaggataag gggcggctcg aactgaattt
      421 tgtcattccg aacatggaac tacagagcgg taagcgcctc cagccgtact acgaccgtgc
      481 ggacaggccg aggataaatg cctggcagac gctggttaat catcattacg ggctgcgtga
      541 cccgaacgca ccggagaaca gacggacgct tgtcacaccg aataatctgc caaaagcgaa
      601 acaggaggcc gcagaagcga ttacacgggg tcttgaggcg ctttaccgtg ccggagcaat
      661 aaaaacgcgt caggatgtca ctgaggcgct cacagcggca ggttttgagg tggtgaggac
      721 aaccagaagc agtatcagta ttgccgaccc ggaggggggg cggaatctca ggctaaaggg
      781 ggcgctgtat gagcaatctt ttacaaatgg gaacggactt cgagaagaaa ctgaaagagc
      841 gagccgcttc taccgagaac atgctgaaca gcgagttcag gaagctagaa gagtctgtag
      901 acaaggcact gagcttaaac aggcagaaaa tcagagacgc tatcagcgaa catacaacat
      961 ccgtgaaaca gcaactggat acgctgagca ccaccgtgag cacgcagctc agcaccacag
     1021 aggccgaact gtcccggcag cagaaaaacc tgctctggca ggtgatcaaa gggcgggtac
     1081 tgtttccggc gctgaccgcg ctcagcgtca cgggcggcat tttcctgggg tgctgggggc
     1141 tgattcagtg gcaggaaagc aggattgcga aaaatatctt gaccatcaga gagcaggaaa
     1201 acacgctggc gaaactcgag gcaaaaacct ggggggtgac gttcgtcaat ggcgagaacg
     1261 ggaaatttct ggtgctgccg gacggggtga aaggggaaaa cacctggacg gtgggcgaca
     1321 agaacgccgt gaggctggtc agggagtaaa cgagtatgac ggaactggaa gggcatttgc
     1381 tgaacgcctt agagcatttg caacaggact atatgcggcg gctgaacgag tgggagagcg
     1441 ccttcgcaga attgcagaag atgcacgcgg ttacgcaaca gaacaacgcg atactgaacg
     1501 agcgtgtcgt gaacttgagt cagcaggtgc agctattagc cggacaagtc gaccgcttga
     1561 gtcggttatt tgtcacgaac aacagataag ggatgaacag gagcgtcagg cgcaggagct
     1621 tgcccggcgc tggcatgaag agcaggaacc cgaagcgcgg atctggcgtg gcccgtcaat
     1681 ggggatgtga ccggggtacc ccctgaaatc gagccagttt gtggcacgcc ctacccggat
     1741 ggcgccaatg ttaatgtacg tacattagca ttaacaatat ccaaacctcc ccattttgcc
     1801 ccgttcgacg gctggaacgt gcgaaactga tcgtcattca gtttcgcgca taactatgca
     1861 tgaggttaaa atttaccagg cgcgatcgcg gcagtttttc gggtggtttg ttgctgtttt
     1921 tacctgtctg ctgccgtgat cgcgctgaac gcgtttaagc ggtacgcgca atgcgacgtt
     1981 atggtaaata cggagtattt taattgcggg cctttgttgt atatctctgt cgtttgtccc
     2041 cggggggaag cattactgct aacaagtccc gtgagaaaac tgcgcaatcg tattgcgcac
     2101 catgaaccta ttctcaaccg caatctggag gatgattttg ctacgataaa gagaattatc
     2161 gcgtatcggt gtcagcattc attggagtgg atgctgaaaa accaggtgtt actccctcta
     2221 cttacactta aacctctcta aaaatataag taagtactat cgagggctat catgaactat
     2281 ggtgccctct gagcatagct acccattact tcttctatgt cattgaataa ttcatttctt
     2341 aactctaagt attctagtaa ctccatttct aatgactctg tcgtgaactt aaatctagaa
     2401 ggatctttga ttgagtttac tagtcttgat aacgctctat atgttttgag tgaaggttgc
     2461 attatagatg gaaagtattt gaaaacctct tcatctctgt tgaaattaac taaaaacttg
     2521 tagtcatcat ttataaatct caagcataca gcgtcaagct tatcgccttt tcttattatt
     2581 atttctttat aggaaagata tttttcagaa gataagtccc acatcaagcg aaaatctctc
     2641 tttgcgaacg agttcgcgtc atcattgaaa agagatgatg tgatggcttt ggatagacct
     2701 tctgtgttta tctctttgtt ggatgggtag taaattttat taattgcttt cattctctca
     2761 aatgatgaat ataggtcatt atgccacctt gttgaaatca ctcctttagt tatattctcc
     2821 ttgctaaaat taagccctgt atcaaaaata aatgcagcca tcagtgaata tgatatgttt
     2881 tttattaact cagacttaaa tggttccgct atggagtagt acagtgataa aataactaaa
     2941 aataaaaggt attgtcttac ctttcccgat gagtatatgt aaaggcgata tttatttatt
     3001 gatagtttta ttttgtatgg aatgatctca tttctgtcat atcctcttcg tggtggagca
     3061 aaacaaaaga aacatgcagc tattaataaa acaaaaaaga tgaacagtgt aagcaagttc
     3121 ggctcctgtg tatgctcact atgcatgatt gtcttgagat ctaacgatcc tgaaggtagt
     3181 cttgtccgta aacgaaaaaa ccgcccagca aggcggcttt tcgaaggttc tcagagcagc
     3241 aactctttga accaaggtaa ctggcttgga ggagcacagc caccaaatct gtcctttcag
     3301 tttagcctta accggcgcat aacttcaaga ctaactcctc taaacggtta ccaatggctg
     3361 ctgccagtgg cgtttttacg tgtctttccg ggttggactc aagacgatag ttaccggata
     3421 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     3481 cctacaccgg gccgagatac caacagcgtg agctatgaga aagcgccacg cttcccgaag
     3541 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     3601 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     3661 ttgagcatct gtttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagcg
     3721 gcgcggcctt cttcctgttc ctggcctttt gctcacatgt ttcttttggc gttatcccct
     3781 gattctgtgg ataaccgcat caccgtcttt gagtgagctg ataccgctcg ccgcagccga
     3841 acgaccgagc gtagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggcatttt
     3901 ctccttacgc atctgtgcgg tatttcacac cgcatatggt gcacttttct tataatccgc
     3961 actgatgcca catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcaggg
     4021 ctgcgccccg acacccgcta aaacccgctg acgcgccctg acgggcttgt ctgctcccgg
     4081 catccgctca caggcaagct gtgaacgtct ccgggagctg catgtttcag ttgctttcac
     4141 cgtcatcgcc gaagcgtgcg aggcaactac ggtagagttc gtcggcgtgg tcgtgaagtg
     4201 attcgcagat gtctgcctgt tcgtccgcgt ccagctcgtt gagtttttcc agcagggtca
     4261 gcgtctggct tctgataaag cgggccatgt taagggctgt tttttcctgt ttggtcatgg
     4321 tcttaaggct ccttaatagg ggatttttta gggggaatga taccgatgaa acgcgagaag
     4381 atgctcacga tacgggttag cgatgaggag cacgcccggt tactggaacg ctgcgagggt
     4441 aaacgactgg cagaatggat gcggcgggtc tgtctggggg agccggttgc gcgcacagga
     4501 aaactgccga cactatcgcc gccattgctg cggcatctgg ctgccatcgg gaacaatctg
     4561 aaccagacag cccggaaggt gaacagcggc cac
//
