LOCUS       AP014955                3897 bp    DNA     circular BCT 15-SEP-2015
DEFINITION  Klebsiella oxytoca plasmid pKO_JKo3_4 DNA, complete genome, strain:
            JKo3.
ACCESSION   AP014955
VERSION     AP014955.1
DBLINK      BioProject: PRJDB4173
            BioSample: SAMD00039250
KEYWORDS    .
SOURCE      Klebsiella oxytoca
  ORGANISM  Klebsiella oxytoca
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1
  AUTHORS   Iwase,T., Ogura,Y., Ishiwata,K., Takeuchi,H., Hayashi,T., Yoneda,M.
            and Mizunoe,Y.
  TITLE     Complete genome sequence of Klebsiella oxytoca JKo3
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3897)
  AUTHORS   Iwase,T., Ogura,Y., Ishiwata,K., Takeuchi,H., Hayashi,T., Yoneda,M.
            and Mizunoe,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-SEP-2015) Contact:Tadayuki Iwase Jikei University
            School of Medicine, Bacteriology; 3-25-8 Nishi-shinbashi, Minato-ku,
            Tokyo 105-8461, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: GS Assembler software v. 2.6
            Genome Coverage       :: 27x
            Sequencing Technology :: 454 GS FLX; Illumina MiSeq
            ##Genome-Assembly-Data-END##
            The genome sequence of JKo3 was determined using 454 GS FLX
            Titanium system. A total of 466,772 single-end reads (162 Mb) and
            141,520 8-kb paired-end reads (44 Mb) were assembled with GS
            Assembler software version 2.6 into 8 scaffold containing 92 gaps.
            The finishing was first performed by in silico analysis using
            GenoFinisher software_ (http://www.ige.tohoku.ac.jp/joho/gf_e/) and
            then remaining gaps were closed by sequencing of gap-spanning PCR
            products using an ABI3130xl DNA sequencer. To correct sequence
            error by resequencing of JKo3 genome, a paired-end library of Jko3
            was constructed using the TruSeq DNA Sample Prep kit and sequenced
            by the Illumina MiSeq platform (2x150 bp). The mapping of obtained
            1,998,167 reads (599 Mb) to Jko3 genome sequence and the
            SNPs-calling were performed using BWA and SAMtools, respectively.
            Total 20 in-dels were corrected by the resequencing. The gene
            identification and annotation were conducted by the Microbial
            Genome Annotation Pipeline (MiGAP).
FEATURES             Location/Qualifiers
     source          1..3897
                     /organism="Klebsiella oxytoca"
                     /mol_type="genomic DNA"
                     /strain="JKo3"
                     /db_xref="taxon:571"
                     /plasmid="pKO_JKo3_4"
     gene            1..513
                     /locus_tag="KOJKO3_p4.1"
     CDS             1..513
                     /locus_tag="KOJKO3_p4.1"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BAS44081.1"
                     /translation="MGGIMIRFVIAAFISILTIKGAFADINVFIAPNDLNYETNSTSEI
                     NHYLDELKKNNTKVDVDKDPKISNELKKYIGIVNDRVSDVFSESEFKKYRGDVCKISIT
                     TGNTAPSDPVLTQVFTYDELADNKKQSCQKLNNELIKKVAKDKGLSYPKVVKDYGSLSI
                     DMIFLFK"
                     /name="hypothetical protein"
     gene            1418..1609
                     /locus_tag="KOJKO3_p4.2"
     CDS             1418..1609
                     /locus_tag="KOJKO3_p4.2"
                     /codon_start=1
                     /transl_table=11
                     /product="rop protein"
                     /protein_id="BAS44082.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDVEADLCEKLHDDA
                     EHLFRTLSSRLDALQDGN"
                     /name="rop protein"
     gene            1609..2010
                     /locus_tag="KOJKO3_p4.3"
     CDS             1609..2010
                     /locus_tag="KOJKO3_p4.3"
                     /codon_start=1
                     /transl_table=11
                     /product="Excl1 protein"
                     /protein_id="BAS44083.1"
                     /translation="MARVSISEAARLTGKSRTTLHRLIKAGDLSTCSGERNAKMVDTSE
                     LLRVFGPFEQPKGEHHSGLVSEQRDTASADHSEQVIRHLKQEIEHLNTLLLSKDSHIDS
                     LKQAMLLLENHQAKLTESPAPWWKFWKKS"
                     /name="Excl1 protein"
     gene            3056..3808
                     /locus_tag="KOJKO3_p4.4"
     CDS             3056..3808
                     /locus_tag="KOJKO3_p4.4"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BAS44084.1"
                     /translation="MGIENSNQGPGWHDHDTPYGTIHSYDGDTGTYGGGGTGGNAGGGH
                     TGLTANTWVDIATTKDYNGRYMSMCLNNLKDHEGSINTMYRDSKGNITVGIGHYLGSAE
                     MAQALPFNRTKVMHVHGDDLESEVAVSKSDIASAFNAYKSNPRDVPSMHLSNADVIGQC
                     IKDVQTTENGLRGLYPGYDRFSDPRKTALVDMGFNLGIPTLKSKFPKFNDAVNRGDWNV
                     AADESHRDLNQRGDKRNKDTADQLRYNR"
                     /name="hypothetical protein"
     Typing          complement(683..1184)
                     /name="rep_cluster_2358"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="86.415"
                     /coverage="99.0"
                     /evalue="1.02e-154"
ORIGIN
        1 atgggtggta ttatgattcg ttttgtgatt gctgctttta tttcaatctt gacaattaaa
       61 ggagcttttg ctgatatcaa tgtttttata gcgcctaacg atctaaatta tgaaacaaat
      121 tctactagtg aaattaatca ttatttagat gagctgaaaa aaaataatac taaagttgat
      181 gttgataaag acccaaaaat tagcaatgag ttaaaaaaat atattggtat tgttaacgat
      241 agagtgtctg atgttttcag tgaaagtgaa tttaagaagt atcgtggcga tgtctgtaaa
      301 atatcaataa caactgggaa tacggctcca tctgatcctg ttttgactca agtgtttact
      361 tatgatgaac tagctgataa taaaaaacaa tcatgtcaaa aattaaataa tgagctaata
      421 aaaaaagttg ctaaagataa gggtttatca tatccgaaag ttgttaaaga ttatggatct
      481 ttatcaattg atatgatctt tttatttaaa taaaaataaa tttacggtgt tgtttatgcc
      541 ttttaaaaaa gtcttttcca atttctattt gtgtaattat attgttggct gcctgccaag
      601 ctaactatat tagggacgta aaaggaggaa ctgttgctcc atcagcttct tcagatataa
      661 gaacgtcgag ttcgcaaccc ataggatctt cttgagatcc ttagcggata gtcgtaatct
      721 ttgctctgta aacgaaaaaa ccacccggca aggtggtttt ttcgaaggtt cattaaccgg
      781 caagtttctg aaccgtggta actggctttc gcagagcaca gaaaccgaat tagttctaac
      841 agtgtagctg tagtttacct cacacttcaa cacttctttg ttctgcgaat gcgttaccag
      901 tggctgctgc cagtggtgat aagtcgtgtc ttttcgggtt ggactcaagt tgatagttac
      961 cgaaaaggcg cagcagtcgg actgaacggg gggttcgtgc atacagtcca gcttggagcg
     1021 aactgcctac ccggaactga gtgtcaggcg tggaatgaga taaacgcggc cataacagcg
     1081 gaatgacacc ggtaaaccga atggcaggaa caggagagcg cacgagggag ccatcagggg
     1141 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca ccactgattt gagcgtcaga
     1201 ttccgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cggccgcctt gcggcctttc
     1261 cttcctctcc tccgttgaac gctctgcttt ctcgagcttt tatacggatg ctcctgccgc
     1321 tcgccgcagc cgaacggcgg aagcgtagcg actgagtgag cgaggaagcg gaaaagagaa
     1381 catccctata ctgagcactg acgctctgag gcctcttatg acgaaacaac agaaaacagc
     1441 gctgaacatg gctaaattca tccagaatca gtctctgctc ttgctggaga agcttaacga
     1501 gcttgatctc gatgttgagg ccgacctttg tgagaaactc cacgatgatg cggagcatct
     1561 tttccgtaca ctgtcctcga gactggatgc attacaggat ggtaactgat ggccagagtg
     1621 tctattagtg aggctgcccg actgacgggt aagagtagaa caacactgca caggttaatt
     1681 aaagcaggtg acctgtccac ctgtagcggt gaacgcaatg caaaaatggt agatacttca
     1741 gagcttttac gtgtctttgg tccctttgag caaccgaagg gtgaacacca ttctggactg
     1801 gtgtctgaac agcgtgatac agcctctgct gaccatagtg aacaggtgat tcgtcatctg
     1861 aaacaggaga ttgaacacct gaatacctta cttttgtcca aggattcgca cattgacagc
     1921 ctgaaacagg ccatgctcct tcttgaaaat caccaggcaa aattaactga atccccggcg
     1981 ccatggtgga aattctggaa aaaatcctga ccccgtcgtg cttcagtttt cctctccggc
     2041 catcccgggg agttcctgaa ccttcccaaa aatcgccaga tctctgaacg ctcttcacca
     2101 ctaaaaatac ccacacacta cgcaaaatta aaattttgca gatttgtatt taatatcagt
     2161 agtttacttt catgaaattt cgttttttaa attttattta catagatccg actctatgac
     2221 gactcgtcat agagtccccg gcgagtcgtc atagagtcgg taacgactca ggactataaa
     2281 aacaatgagt taataacgac ccgactctag acccgactcc ccgtttggtt gttttttaga
     2341 cgcggcaact ctgggtacga ctctagtgag gagtcgccgg tgaatcgtgt ggtgagtcgt
     2401 ccggcgactt gccataacgt gattgccggc cgtcgacgaa cccgattcct gaacgtgctg
     2461 aaaactggtt ttccggctgc gccggcaacg cttcacggcc acgcaataaa ttgcgtcccc
     2521 gttcaccgtt ttcggatggg aagaacgccg gaaacaggac ttaccctggc tgaatggcgt
     2581 gaattttctc gctatgcact tgcgtgcata ctcatgcatg ccttaaaaac agagcctgcg
     2641 cgtttctggc gggttttcgg gtggtttgtt gcctgtttta ccggtttccc gtcagaaacg
     2701 ccctgaggcc gttttagcgg tgcgtacaat tatggcttta tgttaaatca ttgccgcaaa
     2761 cagccacgaa aagcttactt ggcatcggct ctactagcct gttaaaactc atattttgaa
     2821 ctcaatcatt taaatttcgt cttttcaggc gtacaacttc gataactaaa aatttatgtg
     2881 aaatagatat ttatccaaca gttatgtaga taagaataaa acatcaatat ttttttgaga
     2941 gtgtcaagga agttatagtg agaatttttc taaaagtatt gacagattca aagaagatga
     3001 ataaatttat actgtttatt tatacagttg tttttttgtc aaagaggtaa tgtttatggg
     3061 tattgaaaat tcaaatcagg ggccaggatg gcatgatcat gatactcctt atggcactat
     3121 tcacagctat gatggtgata ctggtacgta tggaggtggt ggaactggag ggaatgctgg
     3181 tggtgggcat accggtctta ctgcaaatac atgggtagat atcgcgacaa caaaagatta
     3241 taacggtaga tatatgtcta tgtgtttaaa taatcttaaa gaccatgaag gttcaattaa
     3301 tacaatgtat agagactcta aaggtaatat taccgttggt attggtcatt accttggttc
     3361 tgctgagatg gctcaggcat taccttttaa tcgaacaaaa gttatgcatg tgcatggtga
     3421 tgatcttgaa agtgaggtcg ccgtatctaa gtctgatata gcttcagcat ttaatgcata
     3481 caaatcaaac ccaagagatg ttcctagtat gcatctttca aatgctgatg taataggtca
     3541 gtgtattaag gatgttcaaa ctactgaaaa tggtttgcgt ggactttacc caggttatga
     3601 tcgtttttca gatccacgaa aaacagcatt ggtagatatg ggttttaatc ttggtattcc
     3661 tacgcttaag tctaaatttc cgaagtttaa cgatgcagtt aatcgtggtg attggaatgt
     3721 tgctgcagat gaaagtcatc gagatcttaa tcaaagaggt gataagcgta ataaagatac
     3781 ggcagatcaa ttgcgttata atcggtaatg atttagattt acaactcctg ccatcaaatg
     3841 gcaggagtgt cctcacgata ttaaaactaa gtcatgaggt ttctaatctt atatttt
//
