LOCUS       NZ_CP115388.1           6072 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  NZ_CP115388.1 Escherichia coli strain CUVET20-PYO2 plasmid
            pCUVET20-PYO2.2, complete sequence.
ACCESSION   NZ_CP115388
VERSION     NZ_CP115388.1
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##antiSMASH-Data-START##
            Version      :: 7.1.0
            Run date     :: 2024-06-07 21:39:02
            NOTE: This is a single region extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 6072
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     CDS             1..915
                     /locus_tag="ctg1_1"
                     /transl_table=11
                     /translation="MSAALQYFDENLPHRPYHTDDLAFGLRISGKGRALLARYIQQNQP
                     HAQFWLVFDVDREGAAIDWSDRNAPAPNITVKNPVNGHAHLLYALNIAVRTAPDSSVKA
                     LKYAAAIERSLCEKLCADVNYSGLICKNPFHLEWQVMEWREEAYTLDELADYLDLSASA
                     RRSIDKHYGMGRNCHLFEMTRKWAYRAIRQGWPEFSQWLDAVIQRVEMYNASLPVPLSP
                     PECRAIGRSIAKYTHRNFTPETFAQYVADTHTPEIQAARGRKGGSKSKRSTVATSARTL
                     KPWEALGISRAWYYQLKKRGLVE"
     protocluster    1..6072
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="True"
                     /core_location="[3619:5104]"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      3620..5104
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     cand_cluster    1..6072
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     region          1..6072
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="RiPP-like"
                     /region_number="1"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     CDS             complement(1119..1517)
                     /locus_tag="ctg1_2"
                     /transl_table=11
                     /translation="MRISNIEWLKKRIGFIRKLGEQTARQRQIIDLLDNEAGLTEQERK
                     LLHVLATAEKNDLQAQESERKQAVQKRIEGKKQRRERNHRLFLAAGLLIEAGLVDTKTG
                     ELCYKKDRILQALKELKYDLETSPNPDA"
     CDS             1694..2035
                     /locus_tag="ctg1_3"
                     /transl_table=11
                     /translation="MATYHLSVKFGGKGQAANHADYIERKGEYAKRKDLEYTEHGNMPE
                     WARDNPSHFWQAADQFERANGSTYRELEIALPRELTPEQRLELVQDFVRQEAGERHAGF
                     CHSQSESEH"
     CDS             2064..2618
                     /locus_tag="ctg1_4"
                     /transl_table=11
                     /translation="MMSQRVNDGIDRTPEQYFRRYNARYPERGGAKKDSGSLTLTQQKE
                     QLRELRKRWEVKHNEHMRKHGFERGFIDCRTLKEQGIERRPEVHLGFEAAGRLDDAQRH
                     DIMQKRSEPDYSRHLEQARTVGHDIREWLDTPVKQHELVRRLQHDVAREHGFDMRDRVS
                     RQDRTEPERVKSPSRGFSMGF"
     CDS             2621..3154
                     /locus_tag="ctg1_5"
                     /transl_table=11
                     /translation="MASTEEKLLTLLLSVEHLQHTAMEQQKAVADSLAVARETLRGYRD
                     ISDGVAKQVRDEVRNEVQRLDVAGLIGERISQDFQRLEESVANMKANADALNRDIATIH
                     SNIVQEYNTLKGYSWKWLGGVSVAFLVMFSVLGWGMKTILDRNYNATAAVYQKVVTLED
                     KLAEKPVTGKSRKN"
     CDS             3375..3611
                     /locus_tag="ctg1_6"
                     /transl_table=11
                     /translation="MSGGDGRGHNSGAHNTGGNINGGPTGLGGNGGASDGSGWSSEITH
                     GVAVPVVVFTGEVAPAMAMAGGIVIPVVAAIHP"
     CDS             3620..5104
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: Cloacin"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg1_7"
                     /sec_met_domain="Cloacin (E-value: 1.6e-111, bitscore:
                     360.1, seeds: 4, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAFGFPALAAPGAGTLGISVSGEALSAAIADIFAALKGPFKFSAW
                     GIALYGILPSEIAKDDPNMMSKIVTSLPAETVTNVQVSTLPLDQATVSVTKRVTDVVKD
                     TRQHIAVVAGVPMSVPVVNAKPTRTPGVFHASFPGVPSLTVSTVKGLPVSTTLPRGITE
                     DKGRTAVPAGFTFGGGSHEAVIRFPKESGQKPVYVSVTDVLTPAQVKQRQDEEKRLQQE
                     WNDAHPVEVAERNYEQARAELNQANKDVARNQERQAKAVQVYNSRKSELDAANKTLADA
                     KAEIKQFERFAREPMAAGHRMWQMAGLKAQRAQTDVNNKKAAFDAAAKEKSDADVALSS
                     ALERRKQKENKEKDAKAKLDKESKRNKPGKATGKGKPVNNKWLNNAGKDLGSPVPDRIA
                     NKLRDKEFKSFDDFRKKFWEEVSKDPELSKQFSRNNNDRMKVGKAPKTRTQDVSGKRTS
                     FELHHEKPISQNGGVYDMDNISVVTPKRHIDIHRGK"
     CDS             5106..5369
                     /locus_tag="ctg1_8"
                     /transl_table=11
                     /translation="MELKNSISDYTEAEFVQLLKEIEKENVAATDDVLDVLLEHFVKIT
                     EHPDGTDLIYYPSDNRDDSPEGIVKEIKEWRAANGKPGFKQG"
     CDS             5431..5574
                     /locus_tag="ctg1_9"
                     /transl_table=11
                     /translation="MKKITGIILLLLAAIILAACQANYIRDVQGGTVSPSSTAELTGVE
                     TQ"
ORIGIN
        1 atgagcgccg cgcttcaata cttcgacgaa aatttacccc accgtcccta tcacacggat
       61 gatctcgctt ttggtcttcg catctccggc aaagggcgtg cgcttcttgc gcggtacata
      121 cagcagaacc agcctcatgc acagttctgg ctggtttttg acgttgaccg cgagggggca
      181 gcgattgact ggagcgaccg gaacgctccc gcgccgaaca tcaccgtaaa aaatcctgtg
      241 aacggtcatg ctcacctgct ctacgcgctc aatatcgccg tgagaaccgc gccggacagc
      301 tcggttaagg cgctgaaata cgccgctgcg attgaacgtt cgctgtgtga gaaactttgt
      361 gcggatgtga actacagcgg actgatttgt aaaaatccgt tccacctgga atggcaggtg
      421 atggagtggc gagaggaagc ctataccctc gatgaactgg ctgattatct cgatttgagc
      481 gcctcagcgc gccgtagcat cgataaacat tacgggatgg ggcgaaactg ccacctgttc
      541 gaaatgacgc gcaaatgggc ttacagggcg attcgtcagg gctggcctga attctcacag
      601 tggcttgatg ccgtgattca gcgtgtcgaa atgtacaacg catcgcttcc cgttccgctt
      661 tcaccgcctg aatgtcgggc tattggcagg agcattgcga aatacacgca caggaacttc
      721 acgccggaaa ctttcgcaca gtatgtggct gatacgcaca caccagaaat tcaggctgca
      781 cgcggtcgca agggcggttc taagtctaag cgcagcacag tggctacatc tgcacgcacg
      841 ctgaaaccgt gggaggcatt agggatcagt cgcgcctggt attaccaact gaaaaaacga
      901 ggtctcgtag agtagaccaa ataagcctat atcagataac agcgcctttt tggcgtcttt
      961 ttgagcagca ggttttttgc cgtttttccc cgttcagccc tgcaatggtg ctgttttccg
     1021 catgattgcg ctggtagtgc tcgccgcagt ctcatgaccg agcgtagcga gcgaatgagc
     1081 gaggaagcac cagggaatcg gaacacggat ggaggcactt acgcgtctgg gtttggggat
     1141 gtctccagat cgtacttaag ttccttaagt gcctgtaata tcctgtcttt tttatagcat
     1201 agctcgcctg tttttgtgtc taccagtcct gcttcgatca gtagcccagc cgcgagaaag
     1261 agccggtgat tgcgttcccg tcgttgtttt tttccctcga ttcgtttttg gaccgcctgt
     1321 tttcgttcgc tctcctgtgc ctgtaagtcg tttttttccg ctgttgccag tacgtggagg
     1381 agtttgcgtt cctgttctgt gagtcctgct tcgttgtcga gcaggtcaat aatctgtctc
     1441 tgtcgtgcgg tctgttctcc gagcttcctg atgaatccga tccgtttctt cagccattcg
     1501 atgttgctga tgcgcatgtt cgtggtgctc cctggtgttt tttgcggtgt gttgtggtcg
     1561 tgcagtatat cgcttgcggt cggtgggtgc aggggggtat cttccgtgct aattcgtaaa
     1621 attagaagga agcgcactta tatgtttctc tccttcgtcg agaaacagtg cgctcctgcg
     1681 gagggctgaa accgtggcaa cctatcacct cagcgtcaaa tttggaggca agggacaggc
     1741 tgcaaaccac gccgattaca tcgagcgcaa aggggagtat gcgaaacgga aagatctgga
     1801 atacacggag cacggaaaca tgccggaatg ggccagggat aatccttccc atttctggca
     1861 ggcggctgac cagtttgaac gtgcgaacgg ttcgacttat cgggaactgg aaatcgcgct
     1921 gccccgtgaa ctgacaccgg aacagcgcct tgaactggtg caggattttg tacgtcagga
     1981 agccggcgaa cggcacgccg gtttttgcca ttcacaatcc gaaagcgagc attgacggcg
     2041 gcgaacagcc tcatgcgcat atcatgatga gccagcgtgt gaatgatggc attgaccgta
     2101 caccggaaca gtatttccgg cgctataacg ccaggtatcc cgagcgggga ggtgcgaaaa
     2161 aggattccgg aagcctgact ctcactcagc aaaaagagca gttgcgggaa ttgcgaaaac
     2221 gctgggaggt gaagcacaac gaacatatga gaaagcatgg ctttgaaagg ggcttcattg
     2281 actgccggac gctgaaagaa cagggcattg aacgacgtcc cgaagtgcat ctgggttttg
     2341 aagctgccgg acggcttgat gatgcccagc gtcatgacat catgcaaaaa cggtctgaac
     2401 cggactattc ccgccatctt gaacaggcgc gaacggtcgg gcatgacatc agggaatggc
     2461 tggacactcc ggttaagcaa catgaactgg tcagaaggct tcaacatgat gttgcgcggg
     2521 agcatggctt tgacatgaga gacagagtga gcagacagga ccgaactgaa cctgaacggg
     2581 ttaaatcacc gtcacgtgga ttttctatgg gattctgaac atggccagca cagaagaaaa
     2641 attactcacg ctcctgttat cggtggagca tctgcaacat accgcaatgg agcaacagaa
     2701 agctgttgct gattctctgg ctgtggcaag ggaaacactt cgcggatatc gggatatttc
     2761 tgacggtgtg gcaaaacagg tcagggatga agtcagaaac gaagtgcaga ggctggatgt
     2821 tgcagggctg ataggtgagc gcatcagtca ggattttcag cggctggaag agtcagtcgc
     2881 aaacatgaag gcaaacgcgg atgccctgaa cagggatatt gcgacaattc acagcaatat
     2941 cgtgcaggaa tacaacaccc ttaaagggta tagctggaaa tggctgggtg gggtgtcagt
     3001 ggcctttctg gttatgttca gtgtgctcgg atgggggatg aaaacaattc tcgacaggaa
     3061 ctacaacgcc acagcggcgg tgtatcagaa agttgtgaca ctggaagata aactggcgga
     3121 gaaacctgtc acgggtaaga gccggaaaaa ctgacgcgct tcgtttgtcc aacgcctcac
     3181 ggaccgcgaa aggatttcgc gaaccgttca ccgtttccat gagtgactcc tccgaactgc
     3241 caaaaaatat ccctccctga cttgacagag aaaataatgg cgagtacttt ttgatctgta
     3301 cataaaacca gtggttttat gtacagtatt aactatgtaa ttaattgttt taacgcttaa
     3361 aagagggaat ttttatgagc ggtggagatg gacgtggcca caacagtggc gcacataaca
     3421 caggtggtaa cattaatggc ggccctacgg ggcttggtgg aaatggtggg gcttctgacg
     3481 gctccggatg gagttcggaa ataacccatg gggtggcggt tccggtagtg gtgttcactg
     3541 gggaggtggc tccggccatg gcaatggcgg ggggaatagt aattccggtg gtggcagcaa
     3601 ttcatccgta gcagcaccta tggcatttgg ttttcctgct ttggctgctc ctggtgccgg
     3661 aactcttggt atttccgtat ctggtgaggc tttatctgcg gcaatagcgg atatctttgc
     3721 agccctgaaa ggtccgttta aattcagcgc atggggtatt gcgctttacg gcattctgcc
     3781 atctgaaata gcaaaagatg acccgaatat gatgtcaaag attgtgacgt cattaccggc
     3841 agaaacagtg acgaatgttc aggtcagtac acttccgctg gaccaggcaa ctgtcagcgt
     3901 gacgaaacgt gtgacagatg ttgtgaagga cacacgacag catattgccg tagttgcagg
     3961 tgtgcctatg agtgtgcctg ttgtgaatgc caaaccaaca cgtactccag gtgtatttca
     4021 cgcatcattt cctggtgtcc cttctctgac agtcagcact gtcaaaggcc ttccggtgtc
     4081 aacaaccctt ccccgtggta ttacggagga taaaggccgg actgccgttc ctgcgggatt
     4141 tacctttggt ggtggttcac atgaagcggt gattcgcttt ccgaaagaaa gtgggcagaa
     4201 gccggtttat gtatcagtga cagatgttct taccccagca caggtaaaac aacgtcagga
     4261 tgaggaaaaa cgcctccagc aggaatggaa tgacgcacat ccggtggaag tggctgaacg
     4321 caattatgaa caggcccgcg cagaactgaa tcaggctaat aaagatgttg ccagaaacca
     4381 ggaacgacag gctaaagctg ttcaggttta taattctcgt aaaagtgaac ttgatgcagc
     4441 gaataaaact cttgctgatg caaaggctga aataaaacaa ttcgagcgat ttgcccgaga
     4501 accaatggct gctggtcaca gaatgtggca aatggcaggg cttaaggccc agcgggcaca
     4561 gacggatgta aataataaga aggctgcatt tgatgctgct gcaaaagaaa agtcagatgc
     4621 tgatgttgca ttgagttctg cgttggagcg ccgcaaacag aaagaaaata aagaaaagga
     4681 cgctaaagcc aaattagata aggagagtaa acggaataag ccagggaagg caacaggtaa
     4741 aggaaaacct gtcaataata agtggttaaa taatgcaggt aaagacttag gttctcctgt
     4801 tccagatcgt atagctaata aactacgtga taaggagttt aaaagtttcg atgattttcg
     4861 taagaaattc tgggaagaag tgtcaaaaga tcctgagtta agtaaacaat ttagtaggaa
     4921 caataatgat cgaatgaagg ttggaaaagc gcccaagact agaacccagg atgtttcagg
     4981 gaagagaact tcattcgagc ttcatcatga gaagccgatc agccaaaatg gtggtgtcta
     5041 tgatatggat aacatcagcg tggtaacacc taaaaggcat attgatattc accgaggtaa
     5101 ataatatgga actgaaaaat agtattagtg attacacaga ggctgagttt gttcaacttc
     5161 ttaaggaaat tgaaaaagag aatgttgctg caactgatga tgtgttagat gtgttactcg
     5221 aacactttgt aaaaattact gagcatccag atggaacgga tctgatttat tatcctagtg
     5281 ataatagaga cgatagcccc gaagggattg tcaaggaaat taaagaatgg cgagctgcta
     5341 acggtaagcc aggatttaaa cagggctaaa atatgaatgc cggttgttta aggatgaatg
     5401 actggcattc tttcacaaca aggagtcgtt atgaaaaaaa taacagggat tattttattg
     5461 cttcttgcag ccattattct tgctgcatgt caggcaaact atatccgtga tgttcagggc
     5521 gggacagtat caccgtcgtc aactgctgaa ctgaccggag tggaaacgca gtaacctgca
     5581 atcctcattt gacaagaaca aagcgtgtca ggctgattct gacgcgcttt ttttgttgaa
     5641 gtgccacaaa aattccatgt gggagatggg atctaaaatc tccgtgcaga accttccatc
     5701 aggagggaga aaacttttcg ttttgagccg ttcggtgttc agaacgcacg aaaccgatcg
     5761 cacatcgctt tcgtgcatag ttatgcaggc ccctaaaaac gattctgacg catttttctg
     5821 gttcagcctg gtgtttcctt gtctttttgc gttttttgcg tcagaaagcg attgagcgcg
     5881 ttttagcggt gcgtttaacg ggagttatgg taaatggatc gggttttcgg gaaggttcga
     5941 caggatttgc cgttgggtgt agtgtaagcg actgaaaaac aaacgcccct gaaatcatgt
     6001 ccagatccgg caagattcaa catgaaatac agagggcgtg taggtcgcat aacccggaga
     6061 gaatcgtaac ac
//
