NZ_CP100719.1 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

NZ_CP100719.1

Region	Type	From	To	Most similar known cluster		Similarity
Region 1	NRPS,T1PKS	408	62,093

NZ_CP100719.1 - Region 1 - NRPS,T1PKS

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Location: 408 - 62,093 nt. (total: 61,686 nt) Show pHMM detection rules used

NRPS: cds(Condensation and (AMP-binding or A-OX))
T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

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Gene overview

NRPS/PKS domains

NRPS/PKS modules

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Detailed domain annotation

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NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 407 - 62093:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Ser - Ser - Ile) + (Val - ohmal - D-X)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

ctg1_29

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AMP-binding (549..974): Val

nrpys: Val

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: val (Val)

Stachelhaus prediction details:

Stachelhaus sequence:: DAFWLGGTFK
Nearest Stachelhaus code(s):: Val DAFWLGGTFK (79% 8Å match)
Stachelhaus code match:: 100% (strong)

PKS_AT (1706..1991): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 75.0%; inactive: 70.8%; Propionyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 145.1
inactive: 70.0
Methoxymalonyl-CoA: 57.4
Methylmalonyl-CoA: 46.3
Propionyl-CoA: 39.7
CHC-CoA: 17.1
Ethylmalonyl-CoA: 15.5
fatty_acid: 14.9
Isobutyryl-CoA: 14.1
Acetyl-CoA: 11.9
2-Methylbutyryl-CoA: 10.3
trans-1,2-CPDA: 8.7
Benzoyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0

AMP-binding (3134..3558): X

nrpys: Asp, Asn

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: asp,asn (Asp, Asn)
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DAPRVGQVGK
Nearest Stachelhaus code(s):: OH-Asn or Asn DATKVGEVGK (47% 8Å match)
Stachelhaus code match:: 70% (weak)

ctg1_30

Search NORINE for peptide: strict or relaxed

AMP-binding (38..452): Ser

nrpys: Ser

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Aliphatic chain or phenyl group with -OH (Ser, Thr, Dhpg, Hpg)
Small clusters prediction:: ser (Ser)
Single AA prediction:: ser (Ser)

Stachelhaus prediction details:

Stachelhaus sequence:: DVWHFSLIDK
Nearest Stachelhaus code(s):: Ser DVWHFSLIDK (97% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (1107..1533): Ser

nrpys: Ser

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Aliphatic chain or phenyl group with -OH (Ser, Thr, Dhpg, Hpg)
Small clusters prediction:: ser (Ser)
Single AA prediction:: ser (Ser)

Stachelhaus prediction details:

Stachelhaus sequence:: DVWHFSLIDK
Nearest Stachelhaus code(s):: Ser DVWHFSLIDK (97% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (2194..2609): Ile

nrpys: Ile

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: leu (Leu)

Stachelhaus prediction details:

Stachelhaus sequence:: DGFFLGVVYK
Nearest Stachelhaus code(s):: Ile DGFFLGVVYK (74% 8Å match); Leu DGFFLGVVYK (71% 8Å match)
Stachelhaus code match:: 100% (strong)