LOCUS       NZ_CP066146.1          13882 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  NZ_CP066146.1 Escherichia coli strain ZLWT plasmid unnamed2,
            complete sequence.
ACCESSION   NZ_CP066146
VERSION     NZ_CP066146.1
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##antiSMASH-Data-START##
            Version      :: 7.1.0
            Run date     :: 2024-06-07 19:55:09
            NOTE: This is a single region extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 13882
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     CDS             complement(1..834)
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Orn_Arg_deC_N"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1264:diaminopimelate decarboxylase (Score: 103.7;
                     E-value: 1.2e-31)"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg1_1"
                     /sec_met_domain="Orn_Arg_deC_N (E-value: 3.2e-22, bitscore:
                     70.0, seeds: 15, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAQKITPSKIVKHARELIIKGIESGENSFVIFDVDGALERLDHYR
                     CQLKSFFPNSSIAYSYKSNNLAQWCQIISGKGLYAEVCSVDEMNLAKRDGFKRIVFDGP
                     LKKTSELLKAIEIGALIEVDNIDECKRLNELCKLHKLTCRIHLRLSHYYDDNLSRFGLS
                     ESEAINLLEMLISKSEYLILDGFHLHVGSNLPNAEKICKAIIQYHELILRYMPDDGTLN
                     LGSGIPADSFSASSDNPTPCPEVFFSSIYDTIKNCFGTVCDKWNYIFEPGRHLVED"
     protocluster    1..13882
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="True"
                     /core_location="[2829:3882]"
                     /cutoff="10000"
                     /detection_rule="minimum(1,
                     [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE,
                     Cytolysin_C_RRE, Goadsporin_CD_RRE,
                     Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE,
                     Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE,
                     Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE,
                     Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE,
                     Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE,
                     Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE,
                     Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE,
                     Thiaglutamate_B_RRE, Thiaglutamate_G_RRE,
                     Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE,
                     Thurincin_rSAM_RRE, Trifolitoxin_RRE])"
                     /neighbourhood="10000"
                     /product="RRE-containing"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      2830..3882
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="10000"
                     /detection_rule="minimum(1,
                     [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE,
                     Cytolysin_C_RRE, Goadsporin_CD_RRE,
                     Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE,
                     Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE,
                     Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE,
                     Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE,
                     Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE,
                     Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE,
                     Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE,
                     Thiaglutamate_B_RRE, Thiaglutamate_G_RRE,
                     Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE,
                     Thurincin_rSAM_RRE, Trifolitoxin_RRE])"
                     /neighbourhood="10000"
                     /product="RRE-containing"
                     /protocluster_number="1"
     cand_cluster    1..13882
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="minimum(1,
                     [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE,
                     Cytolysin_C_RRE, Goadsporin_CD_RRE,
                     Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE,
                     Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE,
                     Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE,
                     Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE,
                     Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE,
                     Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE,
                     Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE,
                     Thiaglutamate_B_RRE, Thiaglutamate_G_RRE,
                     Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE,
                     Thurincin_rSAM_RRE, Trifolitoxin_RRE])"
                     /kind="single"
                     /product="RRE-containing"
                     /protoclusters="1"
                     /tool="antismash"
     region          1..13882
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="RRE-containing"
                     /region_number="1"
                     /rules="minimum(1, [Bottromycin_Methyltransferase_RRE,
                     Cyanobactin_D_RRE, Cytolysin_C_RRE, Goadsporin_CD_RRE,
                     Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE,
                     Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE,
                     Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE,
                     Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE,
                     Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE,
                     Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE,
                     Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE,
                     Thiaglutamate_B_RRE, Thiaglutamate_G_RRE,
                     Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE,
                     Thurincin_rSAM_RRE, Trifolitoxin_RRE])"
                     /tool="antismash"
     CDS             complement(819..1622)
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1089:methyltransferase (Score: 60.7; E-value:
                     2.4e-18)"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg1_2"
                     /transl_table=11
                     /translation="MTIKHAEELTEEVILNMNYPDFVALMQQDNTPPGAEYTIDYWIKH
                     GCINKKSHLLDLACSTGFSSRECFKKEGASAEGIDISESAVMVANEKAKKLKANNLLKY
                     YVADACDLPFEDNSFTHVLGGCNFAFIQNRLIALNETHRCLNHMGSMCISNFYYRRKIS
                     DKLINDVYNAINFRPNPLWTLEYWHQFFSERFTLVSEENHEMESQSEDELKADIYDYIF
                     NRNEFTKGLNGSLQNVFFERFLKIRRPLNIQRDYQGVTLQIWRKK"
     CDS             complement(1619..2833)
                     /locus_tag="ctg1_3"
                     /transl_table=11
                     /translation="MNTANILSVNYGAYNAIRMLIGVYHAAFLISTGVTISQLAVLQIV
                     FSVTVLILDFPLSVIADRFYRKQVVVSGVFFTILFYPLCIQSPDMLALVFSEIFYAIGI
                     CCISGAIEGWVLSALDGRNEKFTLCAHLCERVSSFGSVITGAVGILVVYFYDSYKSGYI
                     VSFFLMFVVFIVFLCVKTNGSGGEKEARDSIIKQAKESIGLITGSYNGTYFLSITCMFT
                     IGIQVIYHFWQPIMLSGESIGSLSKRELITLLMCHIGAFSMQYIANYLMPKIDVRCKLY
                     ILVCTLCSFLSSIACILLFMLITDNKVVLGVAIFSIIHGMISTIPVGAQNIFISRLNGN
                     YKNIISGALGLVSLSCRVASISVLGVISVLPGNMPVTTYIFISAIACVLCGCLFLMWSF
                     KKEMV"
     CDS             complement(2830..3882)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RRE-containing: Pantocin_Microcin_RRE"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ThiF"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg1_4"
                     /sec_met_domain="Pantocin_Microcin_RRE (E-value: 5.8e-21,
                     bitscore: 65.6, seeds: 9, tool: rule-based-clusters)"
                     /sec_met_domain="ThiF (E-value: 7.5e-48, bitscore: 154.0,
                     seeds: 54, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDYILGRYVKIARYGSGGLVGGGGKEQYVENLALWENIIKTAYCF
                     ITPSSYTAALETANIPEKDFSNCFRFLKENFFIIPGEYNNSTENNRYSRNFLHYQSYGA
                     NPVLVQDKLKNAKVVILGCGGIGNHVSVILATSGIGEIILIDNDQIENTNLTRQVLFSE
                     DDVGKNKTEVIKRELLKRNSEISVSEIALNINDYTDLHKVPEADIWVVSADHPFNLINW
                     VNKYCVRANQPYINAGYVNDIAVFGPLYVPGKTGCYECQKVVADLYGSEKENIDHKIKL
                     INSRFKPATFAPVNNVAAALCAADVIKFIGKYSEPLSLNKRIGIWSDEIKIHSQNMGRS
                     PVCSVCGNRM"
     CDS             complement(4438..4896)
                     /locus_tag="ctg1_5"
                     /transl_table=11
                     /translation="MTARNAGLSFRSPDDDDSHRTRYSELQQAFDFYNQRLFDGELPEC
                     LITFQRVTQDLFQGKFALSWFDRFSVQVEQQKEMAAVIEQWRETIAQAHQNAEPGLILK
                     RCCQCHRRTGAMITICLLRLLRTSQKGTKSNISVGVWCCCLGEVRTEY"
     CDS             complement(5159..5566)
                     /locus_tag="ctg1_6"
                     /transl_table=11
                     /translation="MSLTLPLMYFQTGADLSKITHHEVHYQDGFVQQAWLQPEIQLFVV
                     CIARKKGSRREIKGGMAVLTSIPASNGAELAKALDNVMRQSDELRPLAGKDENVFIPAR
                     IGISGEEPPTEQELLSVFYQQCLKHGSWGSA"
     CDS             complement(5621..5998)
                     /locus_tag="ctg1_7"
                     /transl_table=11
                     /translation="MSGARFTAMQEKLNVVIEERREDAEREAAERAERERKRQELLQLI
                     AGEGFSPEELLGITEDAPKTRKKTLPKAPPKYQFEENGETKYWSGRGRAPKPIAEALAG
                     GRSLDEFIIKKDASSSAGDEH"
     CDS             6046..6339
                     /locus_tag="ctg1_8"
                     /transl_table=11
                     /translation="MAEHTYVCDRKPDQCGKDSAVRFATRTVWQEAQAQSRAIYQKAPP
                     EIREGFGYSAKVALFRLLSVAFHALTGIKKPAPEVTHSFKVSFAWFFATITT"
     CDS             complement(6205..6477)
                     /locus_tag="ctg1_9"
                     /transl_table=11
                     /translation="MTKHDFVSFVSGELRQGAVRFSLAFNSKGEIVLHWTNKAGIRVWR
                     ILSGNRGKKPSKANLERMSNFRRWLFDARQGMEGYTQQPEQSNLS"
     CDS             complement(6947..7276)
                     /locus_tag="ctg1_10"
                     /transl_table=11
                     /translation="MKVLTFKNDTVSVGDIFVSSWGYEQTNVTFYQVLSVHGKKTVTVR
                     EIRANSEYTDSMVGFKTPVLNDFTGECFKRQIKDFGDELAIKIEDFETAYKTLPEEKHR
                     FSSYY"
     CDS             complement(7369..7584)
                     /locus_tag="ctg1_11"
                     /transl_table=11
                     /translation="MQNKPTPEEVKNARVAAGLTLKEAADIFGYQLNSWQMKESAGKAS
                     RSLSIGEYQYLLLLANMHPSYRLVKK"
     CDS             complement(7574..7819)
                     /locus_tag="ctg1_12"
                     /transl_table=11
                     /translation="MDKDKIIKKNRGNYSYVIRTMDEDGDTVFHVLKYVKTIDKTKSRK
                     TVRKLIMDEKLNLASLMLLDNWVLCDCLTKGDENAE"
     CDS             complement(7864..8187)
                     /locus_tag="ctg1_13"
                     /transl_table=11
                     /translation="MQNYAKSVATEILRQLGGNRFIVMTGAKSFSYFDENGECGLTFRL
                     PSNFAMKGINLVKIKLDFTDTYQVKFSRVRGAEVKDISRFDNIYCDQLARLFTQETGLH
                     TVL"
     CDS             complement(8333..8614)
                     /locus_tag="ctg1_14"
                     /transl_table=11
                     /translation="MTYELAFDRRALKEWQKLGHTIREQFKKKLAERLENPRVPAARLH
                     GHADRYKIKLRASGYRLVYQVIDEKVVLLVIAVGRRESSEVYQIADLR"
     CDS             complement(8604..8855)
                     /locus_tag="ctg1_15"
                     /transl_table=11
                     /translation="MSYQILTTTAASITDLKKNPMGTVAEGEGDAVAILNRNEPAFYCV
                     PPKLYAYYRELAEDAELNAVADERMKNPEIVKVNLDDL"
     CDS             10077..10916
                     /locus_tag="ctg1_16"
                     /transl_table=11
                     /translation="MNLLSVTKKTKIRHRNEINSTFSSLPLSARRILFMAMAQIDSREE
                     LSEGATFRITAREYAFIADIDVTGAYRQLKEGAEELQASVIRIPRNQLLTAQGNQSSGH
                     TKKRGKLPSDAVRLMNLTAFCDYVESEGYIDIAFSHVIEPYITMLKDSYTTQVLISSVR
                     LADQNSNMLYQFIRKQYSSGKKTFFDIEVDVLKDELELFRIDNGEKVYLYQNFKDFNKV
                     FLQKNIEKINQYTEINHLEVKIIERVARRASKLRFSYKIDKESEGLDTRIPYGFRGA"
     CDS             10937..11779
                     /locus_tag="ctg1_17"
                     /transl_table=11
                     /translation="MEQIKLHKQGGAAVIIIPASMIENTGWEDGTLLDVLEINEALHIR
                     RKTGSGALATKSCPSLFSSTMRHVNYDKIIRYNSGLNLLVNELSFSARRVFFICLYYYF
                     ENKLESDKSLLIRADKYAEFVGVDRSVAYKQMKDAADFFSRNKKLITHCDYISNEGLLR
                     VMFSSKTINYITAIDARKYQVTELPLKSALSLQGRYSWPLYQLIKFSHLKSFTIGVDEL
                     KKEIGYIQNQKFSFFKRDGINIAIDEITERTDVKNIKCVNAERQGRRVSKVRFEIEMR"
     CDS             11783..12226
                     /locus_tag="ctg1_18"
                     /transl_table=11
                     /translation="MAKIYQFPQGDERGKFREEIARERKKRFAVKTGSTFVKWLGWTWF
                     YLRLLVASVLHFISVFVLAILGAFKWAVFWLGGILCVITWYHLDHQLWTPQNLTIPVIT
                     ATWIFGLLAEPLMELLNKTMPWHKLLVPEPAGESTENSSETSQ"
     CDS             12366..12722
                     /locus_tag="ctg1_19"
                     /transl_table=11
                     /translation="MKYQVREFITDKYAKAVNILKDNLKENYHVFYGVRLSEILFPASE
                     YGTDAFFKEFELINSVILPLVIFDLTQRKPMMIISFDKIPDASLLEGTNIVVLECTTLA
                     DLLTNDNIGFLYKS"
     CDS             complement(12719..13696)
                     /locus_tag="ctg1_20"
                     /transl_table=11
                     /translation="MRYLTELICQLYRLTDHHKLTAATFKNLADIKLVEPTADADTILQ
                     LENIFSEYSLRYIDRDLAEILSNIITTDKNHSLDFDFNKIQSLTASKSFGCGWNKVING
                     SWFKNLYSWGEGMYPAKNLKAENISDWEDNIWHIEHEGFNPRDPINVKYYSWLDRYVAL
                     NSGGSHHAAMVVYQSVRDKLEYKREAVIEQLSINSNTVEILGQNYYSFIFQIKHPSNKT
                     ENYTSEYEFTDALKDFGQNRHTTLKPVNYVSNIKLAFIPKDALKTNSETFRNWFYSAIS
                     YGKIISLPDYLKNPAHYHTYHYSHELDSITLGNPSRKYKLRGDS"
ORIGIN
        1 gtcttcgacc agatgtctcc ctggttcaaa aatatagttc catttatcac atacagtacc
       61 gaagcaattt tttattgtat catatatcga tgagaaaaat acctccggac atggagttgg
      121 gttatcactg gatgctgaga aagaatctgc aggtatccca ctaccaagat taagagtgcc
      181 atcatctggc atatacctaa gaatcaactc atgatactgt ataatagctt tacatatttt
      241 ttccgcattg ggtagattag agccaacatg taaatggaac ccgtctaaaa tgagatactc
      301 tgacttactg atcaacatct ccagtaaatt aatagcctca ctttcagata agccaaatct
      361 tgataaatta tcatcatagt aatgcgataa tctcaaatga attctacatg tcagcttatg
      421 caacttacat agttcattaa gtcttttaca ttcatcaata ttgtcaactt caattaatgc
      481 cccaatctct attgccttta atagttcact cgtttttttt aaagggccat caaaaacaat
      541 ccgcttaaaa ccgtctcttt tggcgaggtt catttcatca acagagcaca cctctgcata
      601 caaaccttta cctgagataa tctggcacca ttgtgccaga ttatttgact tatatgaata
      661 tgcgatacta ctgttgggga aaaaactctt aagttgacac ctgtaatgat ccagacgctc
      721 cagagctcca tctacatcaa aaatgacaaa tgagttttcg cccgattcaa tccccttaat
      781 aattaactcc cgggcatgtt taacaatttt agatggtgtt attttttgcg ccatatttgc
      841 aatgtaactc cctgataatc tctttggata tttaatggtc tgcgtatttt taaaaatctt
      901 tcaaaaaata cattctgaag tgaaccattc aaaccttttg taaactcatt tctattgaaa
      961 atgtaatcat aaatatcggc ttttaactca tcttcacttt gagattccat ctcatggttt
     1021 tcttctgaaa caagagtaaa tctttcagaa aaaaactgat gccagtattc caaagtccac
     1081 aaaggattgg gtctgaaatt tattgcatta tatacatcat tgataagttt atctgatatt
     1141 ttacgcctgt aataaaaatt tgaaatgcac atgcttccca tgtggtttaa acatcgatga
     1201 gtttcattca aagctattaa ccgattttgt ataaatgcaa aattacatcc accgagaaca
     1261 tgtgtaaaag agttatcttc aaatggtaaa tcacaagcat cagctacata atatttcaat
     1321 aaattattag ccttaagctt ttttgccttt tcatttgcga ccattacagc tgattcagat
     1381 atatcaatac cctctgcaga tgcaccttct tttttgaagc attcacgtga actaaaaccc
     1441 gtggaacatg ctaaatctaa taggtgagac tttttgttaa tacatccatg cttgatccag
     1501 tagtctatgg tgtactctgc tccaggagga gtgttatcct gttgcatcag agcaacaaaa
     1561 tcagggtaat tcatatttaa aataacttct tccgtcaatt cttcagcatg tttaatcgtc
     1621 ataccatctc ctttttaaag gaccacatta agaacaaaca accgcacagt acacatgcaa
     1681 ttgcagagat aaatatataa gtggtgacag gcatattacc tggtaataca gatataactc
     1741 ccaacacaga aatggaagca acacgacatg aaagagaaac aagaccaaga gctcccgaaa
     1801 taatattctt ataattgcca ttaagtctgc ttatgaatat attctgtgct ccaacaggta
     1861 ttgttgaaat catcccatgg attatagaga aaatagctac gccgagaaca accttattat
     1921 ctgttattaa cataaatagt aaaatacagg cgattgaact aagaaatgaa cataatgtac
     1981 aaacaagtat ataaagttta caccttacat ctatctttgg cataagatag tttgctatat
     2041 attgcattga aaatgcacca atatgacaca tcagtaaagt tataagctct ctttttgata
     2101 atgagccaat actttcccca gatagcataa ttggctgcca aaaatgataa attacctgta
     2161 taccaattgt aaacatacat gttatagata aaaaatatgt cccattataa cttcctgtta
     2221 tcaatccaat actctctttt gcctgtttaa taatactatc tcgtgcttct ttttcacctc
     2281 cactgccatt agttttcaca cataaaaaaa cgataaagac aacaaacatc aagaagaacg
     2341 atactatata gccagattta taagaatcat agaagtagac aactaatatt ccaacagcac
     2401 cagttatcac actcccaaag gaagataccc tttcgcataa atgagcacat aaggtaaact
     2461 tttcatttct accatccaga gcacttaaca cccatccctc tatagcccca gatatacaac
     2521 aaataccaat ggcataaaat atttcagaaa aaaccaatgc cagcatatca ggagactgaa
     2581 tacaaagagg gtaaaataat atagtaaaaa atacgccact aactacaacc tgtttcctgt
     2641 aaaatctgtc tgcaatgaca gataaaggaa aatcaagaat cagcactgtc acagaaaaaa
     2701 cgatctgtaa aacagcaagt tgagatatag tgacacctgt agatataaga aatgctgcat
     2761 ggtagacacc aattaacata cgtatagcat tataagctcc atagttcaca gataaaatat
     2821 ttgcagtatt cacattctat ttccacacac agagcaaaca ggcgaacggc ccatattttg
     2881 ggaatgtatt tttatttcat ctgaccagat gcctattctt ttattaagtg ataatggctc
     2941 ggagtatttt ccaatgaact taattacgtc tgccgcacat aatgcagcgg caacattatt
     3001 cacaggtgca aatgttgccg gtttaaatct gctattgatt agcttaatct tatgatcaat
     3061 gttttccttt tctgaaccat atagatctgc cacgactttt tggcactcat aacatcctgt
     3121 tttaccagga acatataatg gaccaaagac tgcaatatca ttaacatacc cagcatttat
     3181 atagggctga tttgctctaa cgcaatattt attaacccag ttaatcagat taaatggatg
     3241 atctgcagaa acaacccata tatcagcttc tggtacctta tgaagatcag tgtaatcgtt
     3301 tatattcaaa gcgatttcag acaccgaaat ttcagagttt ctttttaata actcgcgttt
     3361 tataacttct gttttatttt taccgacatc atcctctgaa aataaaacct gccttgtcaa
     3421 atttgtattt tctatttgat cattatcaat tagtataatt tcgccaatac cagacgttgc
     3481 gagtataaca gacacatgat ttcctatacc cccacaacca agaattacta ccttagcatt
     3541 ttttagttta tcttgcacaa gaacaggatt agcaccataa ctttgataat gtaaaaagtt
     3601 acgagagtaa cgattattct cagtagagtt attatactcg ccaggtataa taaagaaatt
     3661 ttccttaagg aacctaaagc aattagagaa atctttttca ggtatattgg ccgtttccaa
     3721 cgctgcagta taactcgatg gggttatgaa acaatatgcg gtttttataa tattttccca
     3781 aagcgctaaa ttctcaacat attgctcctt tccgcctcca ccaactaatc caccgctccc
     3841 gtatcgagca attttgacat agcgacccaa tatataatcc ataaataaac ctcgcagata
     3901 acttttgtat gccaccctgt acagggtggc atttaatgga catttatatt tttccattag
     3961 tttgcattac cagtacgcat tttacgcctc ctattactgt gattaattca caagtgtttt
     4021 tatcactata aaaaacaata ttccttataa aaatatacaa acatagattt atttcacaaa
     4081 aaaagaacag cacaatcata ttatttgcga ttctacattt ttattctaaa atttattaat
     4141 ttatatattt agacataaat aaccatacag aatataatca tctgcgttta aatcattttg
     4201 taataaatat gctattatgc aataaaagaa tttgatacat ccatcgttca ttacattgga
     4261 aaccaataat cctgattacc agtcacgttg agaagccgct caatgagcgg tggtgcgcta
     4321 cgtgaaatct gagttttttc gggtgggatt ggacacgaga acttatgaga aagcatcagg
     4381 aaatacaatg aaaatagaga ttaattttcg atgaaaggca gctcgcaatc gccgcattca
     4441 atattcagtc cggacttctc ccagacagca gcaccacacc ccgacactga tatttgactt
     4501 tgttcctttt tggcttgttc tcaaaagccg caacaaacag atcgttatca ttgctcccgt
     4561 tctacggtga cactgacagc accgcttcaa tatcaatccc ggttcagcat tttgatgtgc
     4621 ctgagcaata gtttcccgcc attgttcgat gacggcggcc atctcttttt gttgttcaac
     4681 ctggacagag aagcgatcaa accagctcaa agcaaatttc ccctgaaaca gatcctgtgt
     4741 gacgcgctgg aacgtaatca gacactcggg gagttcaccg tcaaaaaggc gctggttata
     4801 gaaatcaaac gcctgttgta gctcgctgta gcgcgttctg tggctatcgt catcatcagg
     4861 actcctgaaa gacaacccag catttcgcgc cgtcatattc attgccactt tctgcaataa
     4921 acacgttatc ttcagtatct accgctatca ctctggggcg ctcatgctga ggggcgcggt
     4981 gaatattttt ccagcaatac acctctccgt cccaaatcag aatcacaccc gaatcagcgt
     5041 gccccgggtt gtgctgacgc catcccctgg caatatcaag cccagggaga gcatcgagcg
     5101 cgtaatattg ctggcaagaa aggtaagcag ggggtatccc tgctttatgt aacagaaatc
     5161 aagcactgcc ccatgagcca tgtttcaggc attgctggta aaagacactt agaagctctt
     5221 gctctgttgg cggttcttcg ccagaaattc caattctggc tgggataaat acgttttcgt
     5281 ctttccctgc cagtggccgc aattcatcag actggcgcat aacgttatcc aacgcttttg
     5341 ccaactccgc tccattgctg gccggaatac tggtcagaac ggccattcca cctttaatct
     5401 cgcgacggga gccttttttc ctggcgatac acacaacaaa tagttgtatt tcaggttgca
     5461 accatgcctg ctgaacaaag ccgtcctgat agtgcacttc atgatgtgtg attttgctga
     5521 gatctgcacc tgtctggaaa tacattaatg gtaatgtcag actcaaaaca tcctcctgtt
     5581 tatcaaaaaa gtctcctgcg taaaccagag gcttattata ttaatgttcg tcgccagcag
     5641 agctggaagc atcttttttg atgataaact catcaagaga gcgaccgcct gccagtgctt
     5701 cagcaatcgg ttttggtgca cgcccacgac cagaccagta tttcgtttca ccattttctt
     5761 caaattgata cttgggcggc gcttttggta acgttttttt acgtgttttt ggcgcatctt
     5821 cggtaatgcc aagcagttct tccggtgaga aaccctctcc ggcgattaac tgaagcagtt
     5881 cctgacgttt ccgttcacgc tctgctcgtt cagccgcttc acgttcagca tcttcacgac
     5941 gttcttcaat aacaacgttg agcttttcct gcatcgccgt gaatctggcg ccgctcataa
     6001 atagtgcttg agcatcaaca aaaatgcaaa aaggctgatg ttgtcatggc tgaacatacg
     6061 tacgtctgcg accggaaacc cgatcaatgc ggtaaggaca gtgccgtcag gttcgctacg
     6121 cgaacggtct ggcaggaagc gcaagcgcag tcccgcgcaa tatatcagaa agcacctccc
     6181 gaaatacggg agggcttcgg ctattcagct aaggttgctc tgttcaggtt gctgagtgta
     6241 gccttccatg ccctgacggg catcaaaaag ccagcgccgg aagttactca ttctttcaag
     6301 gttagctttg cttggttttt tgccacgatt accacttaat atccgccaga ctcgtattcc
     6361 tgctttatta gtccagtgca gaacaatttc tcctttgctg ttaaaagcaa gagaaaagcg
     6421 tacagcgcct tgccgcaatt ctccactgac aaaggacaca aaatcatgtt ttgtcataaa
     6481 gcctccggtt tatttatctg ttctcttttg tgtctttttt tccagtgctt ttctgagtaa
     6541 tgccatatac tcaggaaaga catggaatgg catcaaaggg aaaaccggac gcccaccctt
     6601 ttcaacgtca tttattgctg ataatatgcg ttccgacatt ttattcctcg caatagaatc
     6661 aggtgtcacg gtatcaggtt gccccacaac ctgataccat ctttatcttt actttattaa
     6721 cttctcaatt gcttcttttt ttgctttctc ctgttctaac tttctcctgt tctgatggtg
     6781 ggtatgcaag gtcataccag ttatcaatac aatgaataac tctttcttta gataatccac
     6841 attgctcaaa ccaaaatttt atatttcggt ttaatgatgc ttttgcgcta aactctcttt
     6901 ccattttcag acctttacta aaagggggaa tatcccccct gattaattag tagtaagaag
     6961 aaaatcgatg tttttcttcc ggtagagttt tatacgcagt ttcaaaatct tcgattttga
     7021 ttgccagctc atcaccaaaa tcttttatct gacgcttaaa acattcacca gtaaaatcat
     7081 ttaaaacggg agttttaaag ccgaccattg aatcggtata ttctgaattg gcgcgaatct
     7141 cgcgaacggt gacggttttt ttaccgtgaa cagaaagaac ctggtagaag gttacattgg
     7201 tttgctcata gccccatgat gatacaaaga tatcaccaac agagacagta tcatttttaa
     7261 aagttaatac tttcattttt agctcccgcc cagcttgggc tctggatgaa agagaacctg
     7321 tttccctttc ctgtgtttta ttatacctgc atttgcaggt atggcaagtt atttttttac
     7381 cagcctgtaa gacggatgca tatttgctaa taacaataaa tactgatatt caccaataga
     7441 taaagaacga ctggccttac ctgcactttc tttcatctgc caggaattca gttgataacc
     7501 aaaaatatca gcagcttctt taagagtaag acctgccgca acccgcgcat tctttacttc
     7561 ttcaggtgta ggtttattct gcattttcat ccccttttgt cagacaatca cacaaaaccc
     7621 aattatccag aagcatcaat gatgccaggt tgagtttttc gtccattatc aattttctta
     7681 ctgttttcct gcttttagtt ttatcaatcg tcttaacata ttttaagacg tgaaaaaccg
     7741 tatccccatc ttcatccatc gttctgatta cataggagta attacctctg tttttcttaa
     7801 ttatcttatc tttatccata ttattcctca taactaaaaa aggggcttaa tgcccctttt
     7861 tatctataac acggtatgta accctgtttc ctgtgtaaat aaacgcgcta actgatcaca
     7921 atagatatta tcgaatcttg aaatatcttt aacttcagca ccacgtacac gagaaaattt
     7981 cacctggtac gtatcagtaa aatccagttt aatttttact aagttgatgc ctttcattgc
     8041 aaaatttgac ggcaaacgga aagttaaccc acactcaccg ttttcatcaa agtaagaaaa
     8101 acttttagca ccagtcataa caataaaacg attaccacca agttgacgta aaatctctgt
     8161 ggctacagat ttagcgtagt tttgcatttt taacctcccg cccactttgg gctctggatg
     8221 aaagagaacc tgtttccctt tcttgtgttt tattatacct gcatttgcag gtattgcaaa
     8281 tttttaatgc tctcgctctg actgtaaaca gtcaatcatt tgatgattaa tcttagcgca
     8341 aatctgcgat ctgatagact tcgctgcttt cccttcttcc aacggcaata acgagtaaaa
     8401 cgactttctc atcaatgact tgatatacaa gtctgtagcc agatgcacga agtttaattt
     8461 tatagcgatc agcatgacca tgtaaccggg ctgcgggtac gcgtggattt tccagccgtt
     8521 ctgccagttt ctttttgaat tgttcacgga tggtgtggcc gagtttctgc cattccttca
     8581 gtgctctacg gtcaaaagcc agttcatagg tcatccaggt taaccttcac aatttccggg
     8641 tttttcatgc gctcatcagc aacagcgttt aactcagcat cttcagcgag ttcccgatag
     8701 taggcgtaaa gttttggtgg aacgcaatag aacgccggtt cgtttcggtt caggatcgca
     8761 acagcgtccc cttcaccttc agctacggtt cccataggat tttttttcag gtcagtaata
     8821 ctggccgctg tggttgtcag aatttgatag ctcataagcc ctccgcttgt ctcattggca
     8881 taatagcatc tttaaaagca ctcttaaaag tgcttatgtt gagaaaaaca tcagaacagg
     8941 tgagatatgg ctctttccga catcgcctta aaacgctctg taacgcgttc tgagcgtttt
     9001 ttcccgtttc gttgtagtca gctacggttt aacggctttt aacgccgctc aacgcttttt
     9061 agcggccttg ttgctatgat ccggatagga gttcaatctt gatcaaaatt tcctcaaggc
     9121 aacgactgtt ttgtaaatac cctctaaaac gcgctgtatc gcattctgag cgattttttc
     9181 gatttcacat ggattttctt atggttgtac tgaacgaagc tgcagaaagc gattcagggc
     9241 ttctcagtgg aagatccaaa aaggatcaat caacggatcg tatgatctga actcttctgg
     9301 ttttgagaaa ggagacaccg ctcgccgcag ccgaacgacc gagcgtagcg agttagtgag
     9361 cgaggaagcg gaacagcaca gggataacat caggcaatta cgcacagtgg ttaaaaagcg
     9421 agcatattgt ttttatgtgt ggttgtcctg gttcagagtg atctgtcctc tgggtttacg
     9481 caaagttatc cacaggcact gtgaattttt tgttttgagc agagttatcc acagattttt
     9541 tgtgtttttt tgcttattaa acttcttgtt tttaagatct taaatgtaga tcggtagatc
     9601 tactttaaga tctttaactt actttaactt attctgcaaa cattgtgcca gcttttttat
     9661 caataatttc attaagatag gtgttgtttt ttgtcgcctc aaaatgaaag gtggatgggt
     9721 acgcactgaa aggtggatgg gtacgcactg taattttcat gaaaggtgga tggctgcgca
     9781 ctgaaaggtg gatgggtacg cactgaaagg tggatgggta cgcattttac tgtttttatt
     9841 cagatattca tctctttgca tcttcatttc tttagttctt tatagcgttc caatccaacg
     9901 gtaaaagtta aaaggtggat acctgcgcat ttctatcata ttgaatataa atgataaaaa
     9961 ttctttcatg tgatctgtgt tttggtggaa atgtataagg tggataaacc actttacaca
    10021 tttccgcttt tgtggttatg atttcaccat caggacttaa aaaacacagg taatgtatga
    10081 acttactgag cgttacaaag aaaacaaaaa ttcgccacag aaatgaaatt aacagcacat
    10141 tctcatcgtt gcctttgtct gccagaagaa ttttgtttat ggctatggcg cagattgatt
    10201 cacgtgaaga gttatctgag ggagcaacct ttcgtattac ggctcgggaa tatgcattta
    10261 tcgctgatat tgatgtaaca ggtgcttacc ggcagttaaa agagggggca gaagaacttc
    10321 aggctagtgt tattcgcatt cctcgaaatc aacttctcac agctcagggc aatcaaagct
    10381 ctggacatac gaagaagaga ggtaaattac cttctgatgc agtcaggctt atgaacctga
    10441 ctgcgttttg tgattatgtt gaaagtgaag gatatattga tattgctttt tcacatgtca
    10501 ttgagccata tatcacgatg ttaaaggatt cttacactac tcaggttttg atatcgtctg
    10561 taagactcgc tgatcaaaat tcaaatatgc tttatcagtt tatcaggaaa caatacagca
    10621 gtggtaaaaa gacatttttc gacattgaag tagatgtatt aaaggatgaa cttgaattat
    10681 tcagaataga taatggcgaa aaagtttacc tttatcagaa ttttaaggat tttaataagg
    10741 tttttctgca aaaaaacatt gaaaaaataa atcaatatac agagattaat catctcgaag
    10801 ttaaaattat tgagagagtc gcaagaagag catcaaagct aagattttcg tataaaatag
    10861 ataaagaaag cgaaggactt gataccagaa taccgtatgg tttcagaggt gcttaatata
    10921 taaaggtaaa tacactatgg agcaaataaa attacataaa caaggtggtg cggcagtaat
    10981 tatcattcct gccagtatga tagaaaatac cggatgggaa gatggaacct tattggatgt
    11041 actagaaata aatgaggctc ttcatataag aagaaagaca ggatcaggag ctttagctac
    11101 taaatcttgt ccttctcttt tttcatctac tatgaggcat gttaactacg ataaaatcat
    11161 tagatataat agcggattaa atctgttagt taatgaactg tcattttcgg caagaagggt
    11221 cttttttatt tgtttatatt attactttga aaataaactt gaatcggata agtcgctatt
    11281 gatccgggct gataagtatg cagaatttgt tggtgttgat cgttctgtcg catataagca
    11341 aatgaaggat gcagcagatt tcttttcaag aaataaaaaa ttaattacac attgtgacta
    11401 tatttcgaat gagggattgc ttcgtgttat gttttcgtct aagacgataa attatattac
    11461 agcgatagac gcccgtaaat atcaggttac cgagcttcct ctaaaatctg ctttgtctct
    11521 tcaaggaaga tactcatggc ctttatatca actgataaaa tttagccatc ttaaatcttt
    11581 cactataggt gtagatgaac ttaagaaaga aataggttat attcaaaatc aaaagttttc
    11641 tttctttaaa agagatggaa tcaatatagc tattgatgaa ataacagaaa ggacagatgt
    11701 taaaaacatt aaatgtgtta atgctgaaag gcagggtaga agagtcagca aagtccgttt
    11761 tgagatcgaa atgaggtaac tcatggctaa aatctatcaa ttcccccaag gggacgaacg
    11821 cggtaaattc agggaagaaa tcgccagaga acgtaagaaa cgttttgcgg tgaaaacagg
    11881 gagcactttt gttaagtggc ttggatggac ctggttttat cttcgtcttc tggtcgccag
    11941 tgttttacat tttatctctg tttttgtgtt agccattctt ggcgcgttta aatgggctgt
    12001 gttctggctg ggtggcattc tgtgtgtcat aacctggtat catctcgatc accagttatg
    12061 gacgccacaa aatctgacta taccggtgat tacggcaacc tggatttttg gtctgttggc
    12121 tgaaccgctg atggagctgc tcaacaaaac catgccctgg cacaagttac tggttcccga
    12181 acctgcgggc gaaagcacag agaacagcag cgagacgagc cagtaatgac gctacttagt
    12241 cgttatgcca gcccctgcct tttcactgcg atgcaggata gggcaaacac cgcaaaatga
    12301 cgtctctgac gccattccgc aacgtttgcc cggtgaccta cagtcggttc ttgtcggaga
    12361 attttatgaa atatcaggtg agagaattta ttactgataa atatgcgaag gctgttaata
    12421 ttttaaagga taaccttaaa gaaaactacc atgtttttta tggtgtgaga ttaagtgaga
    12481 ttctttttcc agccagtgag tatggcactg atgctttctt taaagagttt gaattaatta
    12541 acagtgtaat acttccatta gtaatctttg atttaacaca acgcaagcca atgatgatta
    12601 tcagcttcga taagatacct gatgccagtt tgttggaagg aacaaatatt gtggttcttg
    12661 aatgtactac cctcgccgat cttctgacaa atgataacat tggtttttta tataaaagct
    12721 aagaatctcc tctcagttta tattttctgc ttgggttgcc aagtgttatc gagtctagtt
    12781 catgtgagta atgatatgta tgataatgtg ctggattttt cagataatca gggagtgata
    12841 ttattttccc gtagctaatt gcagaataaa accagttgcg gaatgtctcg ctgtttgttt
    12901 ttaaagcatc tttgggaata aatgctaatt ttatgttaga aacgtaatta acaggtttaa
    12961 gtgtggtatg cctattttgt ccaaagtctt ttaaagcatc tgtaaattca tattctgatg
    13021 tataattttc tgtcttatta ctagggtgtt ttatctggaa tatgaatgaa tagtagttct
    13081 ggccaagtat ttcaacggtg tttgaattta tgctcagttg ttcaataacc gcttctcttt
    13141 tatactctaa tttgtctctg acagattgat atacaaccat tgctgcatga tgtgaaccac
    13201 cactgtttaa agctacatat cggtcaagcc aagagtaata tttaacatta ataggatcgc
    13261 gagggttgaa accttcatgc tcaatgtgcc atatattatc ctcccagtct gaaatgtttt
    13321 cagctttcag gtttttggcg ggatacatcc cttcccccca tgaataaagg tttttgaacc
    13381 aactaccatt tattactttg ttccatccac aaccgaaact tttcgaggca gtcagagact
    13441 gtatcttgtt aaaatcaaaa tccagagagt ggtttttatc tgtagttatt atgttactca
    13501 gaatttcagc cagatccctg tcaatatatc ttagtgagta ttcgctaaat atattttcaa
    13561 gctgcaatat ggtgtctgca tccgctgtag gttcaactaa cttaatatca gcaagatttt
    13621 taaacgttgc agcggtcagt ttatggtgat ctgtcaacct gtagagttga cagattaatt
    13681 cggttaaata tctcatagat aatcatccat acttctatgg ttagaatttt gccgattcca
    13741 gtaaccagtg attctttatg ttttcatcct ggctgttgta catcgccagc gctgctttga
    13801 gaacggttgt tttgctctgc cctgttctga tagcttcagc ttccagtatg tcctcaaaat
    13861 gggctgaaag tctgaagttc at
//
