NZ_CP064203.1 - 8 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

NZ_CP064203.1

Region	Type	From	To
Region 1	NAPAA	356,482	390,480
Region 2	NRP-metallophore	621,625	674,827
Region 3	redox-cofactor	1,559,325	1,581,515
Region 4	NI-siderophore	1,683,615	1,720,058
Region 5	betalactone	1,995,250	2,024,331
Region 6	arylpolyene	2,532,650	2,573,879
Region 7	arylpolyene	2,855,618	2,899,220
Region 8	NRPS,hserlactone,arylpolyene	3,300,415	3,383,644

NZ_CP064203.1 - Region 1 - NAPAA

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Location: 356,482 - 390,480 nt. (total: 33,999 nt) Show pHMM detection rules used

NAPAA: (AMP-binding and TIGR02353)

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NRPS/PKS domains

NRPS/PKS modules

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Detailed domain annotation

Selected features only

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NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 356481 - 390480:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Lys)

Direct lookup in NORINE database: strict or relaxed

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NRPS/PKS substrate predictions

ctg1_314

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AMP-binding (29..418): Lys

nrpys: Lys

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DMEHNGTVSK
Nearest Stachelhaus code(s):: Lys DAEDNGTVSK (56% 8Å match)
Stachelhaus code match:: 80% (moderate)

NZ_CP064203.1 - Region 2 - NRP-metallophore

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Location: 621,625 - 674,827 nt. (total: 53,203 nt) Show pHMM detection rules used

NRP-metallophore: (VibH_like or Cy_tandem or (cds(Condensation and AMP-binding) and ((IBH_Asp and not SBH_Asp) or IBH_His or TBH_Asp or CyanoBH_Asp1 or CyanoBH_Asp2 or IPL or SalSyn or (EntA and EntC) or (GrbD and GrbE) or (FbnL and FbnM) or PvdO or PvdP or (Orn_monoox and not (KtzT or MetRS-like)) or Lys_monoox or VbsL)))

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

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NRPS/PKS substrates

NRPS/PKS substrate predictions

ctg1_549

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AMP-binding (31..417): Thr

nrpys: Thr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Aliphatic chain or phenyl group with -OH (Ser, Thr, Dhpg, Hpg)
Small clusters prediction:: thr (Thr)
Single AA prediction:: thr (Thr)

Stachelhaus prediction details:

Stachelhaus sequence:: DMFSAGLIWK
Nearest Stachelhaus code(s):: Thr DMFVAGLIWK (91% 8Å match)
Stachelhaus code match:: 90% (moderate)

ctg1_559

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AMP-binding (37..442): diOH-Bz

nrpys: diOH-Bz

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aromatic (Phe, Tyr, diOH-Bz, Ph-Gly, bOH-Tyr)
Large clusters prediction:: Hydroxy-benzoic acid derivates (diOH-Bz, Sal)
Small clusters prediction:: Hydroxy-benzoic acid derivates (diOH-Bz, Sal)
Single AA prediction:: dhb (diOH-Bz)

Stachelhaus prediction details:

Stachelhaus sequence:: MLPSQGVVNK
Nearest Stachelhaus code(s):: diOH-Bz PLPSQGVVNK (94% 8Å match)
Stachelhaus code match:: 90% (moderate)

NZ_CP064203.1 - Region 3 - redox-cofactor

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Location: 1,559,325 - 1,581,515 nt. (total: 22,191 nt) Show pHMM detection rules used

redox-cofactor: (cds(PF04055 and (TIGR04085 or SPASM)) and (TIGR02111 or TIGR03966) and minimum(1, [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE, Cytolysin_C_RRE, Goadsporin_CD_RRE, Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE, Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE, Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE, Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE, Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE, Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE, Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE, Thiaglutamate_B_RRE, Thiaglutamate_G_RRE, Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE, Thurincin_rSAM_RRE, Trifolitoxin_RRE]))

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

NZ_CP064203.1 - Region 4 - NI-siderophore

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Location: 1,683,615 - 1,720,058 nt. (total: 36,444 nt) Show pHMM detection rules used

NI-siderophore: IucA_IucC

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		NT	AA		NT	AA

NZ_CP064203.1 - Region 5 - betalactone

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Location: 1,995,250 - 2,024,331 nt. (total: 29,082 nt) Show pHMM detection rules used

betalactone: (HMGL-like and AMP-binding)

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NZ_CP064203.1 - Region 6 - arylpolyene

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Location: 2,532,650 - 2,573,879 nt. (total: 41,230 nt) Show pHMM detection rules used

arylpolyene: (APE_KS1 or APE_KS2)

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Detailed domain annotation

Selected features only

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NRPS/PKS substrates

NRPS/PKS substrate predictions

NZ_CP064203.1 - Region 7 - arylpolyene

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Location: 2,855,618 - 2,899,220 nt. (total: 43,603 nt) Show pHMM detection rules used

arylpolyene: (APE_KS1 or APE_KS2)

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Detailed domain annotation

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NRPS/PKS substrates

NRPS/PKS substrate predictions

ctg1_2764

AMP-binding (114..289): X

nrpys: Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: IYFIFSVGIK
Nearest Stachelhaus code(s):
Stachelhaus code match:: 0% (weak)

NZ_CP064203.1 - Region 8 - NRPS,arylpolyene,hserlactone

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Location: 3,300,415 - 3,383,644 nt. (total: 83,230 nt) Show pHMM detection rules used

NRPS: cds(Condensation and (AMP-binding or A-OX))
hserlactone: Autoind_synth
arylpolyene: (APE_KS1 or APE_KS2)

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NRPS/PKS domains

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Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Detailed domain annotation

Selected features only

Show module domains

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NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 8, location 3300414 - 3383644:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Gly)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 9, location 3300414 - 3344374:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Gly)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

ctg1_3173

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AMP-binding (491..872): Gly

nrpys: Gly

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Tiny, hydrophilic, transition to aliphatic (Gly, Ala)
Single AA prediction:: gly (Gly)

Stachelhaus prediction details:

Stachelhaus sequence:: DILQLGVVWK
Nearest Stachelhaus code(s):: Gly DILQLGVVWK (85% 8Å match)
Stachelhaus code match:: 100% (strong)

ctg1_3176

CAL_domain (162..486): pk

minowa: NH2

Prediction, score:

NH2: 119.3
Acetyl-CoA: 60.3
fatty_acid: 21.4
shikimic_acid: 0.0
AHBA: 0.0

ctg1_3184

AMP-binding (38..443): X

nrpys: Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: AYYASAHMVK
Nearest Stachelhaus code(s):
Stachelhaus code match:: 0% (weak)