LOCUS       NZ_CP063463.1           6747 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  NZ_CP063463.1 Escherichia coli strain EM22-18-53 plasmid
            pEM22-18-53_4, complete sequence.
ACCESSION   NZ_CP063463
VERSION     NZ_CP063463.1
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##antiSMASH-Data-START##
            Version      :: 7.1.0
            Run date     :: 2024-06-07 17:41:52
            NOTE: This is a single region extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 6747
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     CDS             complement(1..2091)
                     /locus_tag="ctg1_1"
                     /transl_table=11
                     /translation="MAIYHLSMKIISRKNGYSAVASAAYRSGSVIPDDRTGLIHDYTRK
                     RGVDDAVILTPANAPSWCGDRSVLWNAVEKAEQRRNSQLAREIELAIPREISREAAREA
                     VLAFVRENFVSRGMIADVAFHHMDRTNPHAHIMLTTRAVGETGFAGKVRDWNDRALAET
                     WRASWADHANRALANAGYQEEIDHRSYERQGLEKAPGLHLGKAACAMEKRGMETERGEQ
                     NRLINSLNLEIQVSRTRLAVIQVQENVRKRELSDAARRAAEALNLTIPAANASADTLRE
                     FIATLPQECGNAWEMTPEFVAMNGKANDIKREGNALVKERDILEKEMTGLRKSRPVASI
                     LSELPLMTWAEPEYRKRQLRFWKLEKQIESLLRTYRAVKERDIPARRQAFETQWNTWIS
                     PGMAELKEKLSAREAERRREEAEAEARRKEQEYDARLKHHDNHRLSRETALAGVITELG
                     RAREPGTGRITRYMMLSNRAGEFTVWGDELAHYPQSVHDPVNVYLSPGGAVMVSDIRER
                     MPESHETMARPERVRMYSGATVRHVLEQMRQGWPSYGFPALPHHWPDNFYFSDDRRPAA
                     SPLPSAHRVDVTAYAAPEQLMPVVFSTERNSRTLNLLLCEGPEEMLVGFVRLEDELRPV
                     LALPSPDYSHLMVSIITENGIHLAGYGEAINRDADTPYPPEPKLMQFRLKGCQDTLFAA
                     INK"
     protocluster    1..6747
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="True"
                     /core_location="[4638:6387]"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      4639..6387
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     cand_cluster    1..6747
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     region          1..6747
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="RiPP-like"
                     /region_number="1"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     CDS             2270..2545
                     /locus_tag="ctg1_2"
                     /transl_table=11
                     /translation="MSRTLEQKIAEAEARLQRLKAKSRSLDTAQKVIVGAAMLARVRRP
                     EEAQLRAFLLQFLRKEVTRQADMNRLQPLINELEKLPRPPAKPQNH"
     CDS             complement(2751..3677)
                     /locus_tag="ctg1_3"
                     /transl_table=11
                     /translation="MSAALQYFDENLPHRPYHTDDLAFGLRISGKGRALLARYIQQNQP
                     HAQFWLVFDVDREGAAIDWSDRNAPAPNITVKNPVNGHAHLLYALNIAVRTAPDSSVKA
                     LKYAAAIERSLCEKLCADVNYSGLICKNPFHLEWQVMEWREEAYTLGELADYLDLSASA
                     RRSIDKRYGMGRNCHLFEMTRKWAYRAIRQGWPAFSQWLDAVIQRVEMYNASLPVPLSP
                     AECRAIGKSIAKYTHRNFTPETFAQYVADTHTPEIQAARGRKGGKIGGAKSKRGAVATS
                     ARTLKPWETLGISRAWYYQLKKRGLVE"
     CDS             complement(4174..4317)
                     /locus_tag="ctg1_4"
                     /transl_table=11
                     /translation="MKKITGIILLLLAVIILAACQANYIRDVQGGTVSPSSTAEVTGLA
                     TQ"
     CDS             complement(4379..4636)
                     /locus_tag="ctg1_5"
                     /transl_table=11
                     /translation="MELKNSISDYTETEFKKIIEDIINCEGDEKKQDDNLEHFISVTEH
                     PSGSDLIYYPEGNNDGSPEAVIKEIKEWRAANGKSGFKQG"
     CDS             complement(4639..6387)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: Cloacin"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg1_6"
                     /sec_met_domain="Cloacin (E-value: 1e-99, bitscore: 321.3,
                     seeds: 4, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSGGDGRGHNTGAHSTSGNINGGPTGIGVSGGASDGSGWSSENNP
                     WGGGSGSGIHWGGGSGRGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLA
                     VSISASELSAAIAGIIAKLKKVNLKFTPFGVVLSSLIPSEIAKDDPNMMSKIVTSLPAD
                     DITESPVSSLPLDKATVNVNVRVVDDVKDERQNISVVSGVPMSVPVVDAKPTERPGVFT
                     ASIPGAPVLNISVNNSTPAVQTLSPGVTNNTDKDVRPAGFTQGGNTRDAVIRFPKDSGH
                     NAVYVSVSDVLSPDQVKQRQDEENRRQQEWDATHPVEAAERNYERARAELNQANEDVAR
                     NQERQAKAVQVYNSRKSELDAANKTLADAIAEIKQFNRFAHDPMAGGHRMWQMAGLKAQ
                     RAQTDVNNKQAAFDAAAKEKSDADAALSAAQERRKQKENKEKDAKDKLDKESKRNKPGK
                     ATGKGKPVGDKWLDDAGKDSGAPIPDRIADKLRDKEFKNFDDFRRKFWEEVSKDPELSK
                     QFNPGNKKRLSQGLAPRARNKDTVGGRRSFELHHDKPISQDGGVYDMDNLRITTPKRHI
                     DIHRGQ"
     CDS             complement(6619..6747)
                     /locus_tag="ctg1_7"
                     /transl_table=11
                     /translation="MEEMPDYLFRQLGFNQTWHEWKREEQRRQQQRRPGHFRGMSM"
ORIGIN
        1 tttgttgatg gcagcaaaaa gcgtgtcctg acagccctta agcctgaact gcatcagctt
       61 cggttccggt gggtacggag tatcagcatc acggtttatg gcctcaccgt aacctgccag
      121 gtgtatcccg ttctctgtga tgatgcttac catcagatga ctgtaatccg gcgacggaag
      181 cgcaagaacg ggacgcagct cgtcctccag gcgcacaaaa ccgacaagca tttcctccgg
      241 cccttcgcac aacagcagat tcagcgtcct gctgtttcgc tctgtcgaaa agacaacggg
      301 catgagttgc tccggtgccg cataagcggt gacgtccacc cggtgcgctg acggcagcgg
      361 agaggctgcg ggcctgcggt cgtcgctgaa ataaaaatta tccggccagt gatgcggcag
      421 cgccggaaaa ccgtaagagg gccacccctg gcgcatctgt tccagtacat gccggaccgt
      481 cgcaccggaa tacattctca cacgctcagg ccgcgccatc gtctcatgag attctggcat
      541 tctctcacgt atatccgaga ccatcacagc cccgcctggc gacaggtaaa cattcaccgg
      601 gtcatgaaca ctctgggggt aatgcgccag ctcatcaccc cataccgtga attctccggc
      661 tctgttactc agcatcatgt agcgggttat cctgcccgtt cccggctctc tggcacgccc
      721 cagctccgta ataaccccgg ctaatgccgt ttcacggctc aggcgatggt tatcatggtg
      781 tttcagccgt gcgtcatatt cctgttcctt ccggcgcgct tccgcttcgg cttcctccct
      841 gcgccgctcc gcttcccgtg ctgacagttt ttctttcagc tctgccattc ccggcgaaat
      901 ccacgtattc cactgcgttt caaaggcctg acgacgggcg ggaatgtccc gttctttcac
      961 ggccctgtag gtgcggagaa gagattcaat ctgtttctcg agtttccaga agcggagttg
     1021 tcttttgcgg tattccggtt cagcccatgt catcagggga agctctgaca ggatggacgc
     1081 taccggacgg gattttctga gtccggtcat ctctttttcg agaatatccc gctctttcac
     1141 cagcgcattc ccctcacgtt tgatgtcgtt tgccttcccg ttcatcgcca cgaactccgg
     1201 tgtcatctcc catgcgttcc cgcattcctg cggcagtgtg gcaatgaatt cccgcagggt
     1261 atccgctgag gcattcgcgg cgggaatggt cagattaagg gcttccgctg cacgacgtgc
     1321 ggcatcgctg agttcccgtt tccgcacgtt ctcctgtacc tggatgactg ccagccttgt
     1381 gcgggaaacc tgtatttcca ggttaaggct gttaatcagg cggttctgct caccgcgttc
     1441 tgtttccatg ccccgttttt ccatcgcaca ggcagccttt ccgaggtgaa ggcccggcgc
     1501 tttctccagt ccctgacgct cgtatgaacg atggtctatc tcttcctggt agccggcgtt
     1561 cgcaagcgct ctgttcgcat ggtcagccca tgatgcgcgc cacgtctcgg cgagtgcccg
     1621 gtcgttccag tccctgacct ttcctgcgaa tcccgtttcc ccgacagctc tcgtggtcag
     1681 catgatgtgt gcatgggggt tggtccggtc catatgatgg aacgccacat cggcaatcat
     1741 gccccgactg acaaagtttt cccggacaaa agcgagaacg gcctcccgtg cggcctcgcg
     1801 ggaaatctca cggggaatgg cgagttcaat ctcccttgcc agctgggagt tccggcgctg
     1861 ttcggctttc tcgaccgcat tccagagaac ggaacggtca ccacaccagg acggtgcatt
     1921 cgcaggggtg agaatgaccg catcatcaac gccgcgttta cgggtgtaat cgtggattaa
     1981 tccggtacgg tcatcgggta tgacagagcc ggaacggtag gcagcagaag caacggcact
     2041 gtagccgttt tttcgcgaaa tgattttcat gctgagatga taaatcgcca tttcttccgt
     2101 aatgcctttt tgccgttttc tttcagcccc gcagggcgca cacaatcgaa acgaagtgaa
     2161 gattgtataa gtgcgcactt cgtgatgtgc cgtgcattat gccagttcag ttttgcaact
     2221 accagtgacg cgctaaaata cgatttttca ccgcgcacgg agtcacgaaa tgagcagaac
     2281 actcgaacag aagattgccg aagctgaggc cagattacag cgcctgaagg cgaagagccg
     2341 gagcctggac acggcgcaaa aagtcattgt gggggctgcc atgctggcga gggtacggag
     2401 gccggaagaa gcgcaactgc gtgcgttcct gctccagttt ctgcgaaagg aagtcacaag
     2461 acaggccgat atgaaccgac tacaaccgct gattaacgag ctggagaagc tacccagacc
     2521 accggcgaaa ccgcagaacc actaagcccc ggcgcgtaag agccacatac accaccggac
     2581 gcctttgcgc ttcctcgctc attcgctcgc tacgctcgga catgagactg cggcgagcgc
     2641 tacaacctca atcacgcgga aagccgcacc attgcaggga ggaaacgaaa aagccaccaa
     2701 acgccaacag ctcaaaaaag cgctgttatc tgatataggt tttatttggt ctactctaca
     2761 agacctcgtt ttttcagttg gtaataccag gcgcggctaa ttccaagagt ctcccacggt
     2821 ttcaacgttc gcgcagatgt ggcaacagca ccacgcttag acttagcacc tccaattttc
     2881 ccgcccttgc gaccacgtgc agcctgaatt tctggcgtgt gcgtatcagc cacatactgt
     2941 gcgaaagttt ccggcgtgaa gttcctgtgc gtgtatttcg caatgctctt gccaatagcc
     3001 cgacattcag ccggagaaag cggaacggga agcgatgcgt tgtacatttc gacacgctga
     3061 atcacggcat caagccactg tgagaatgct ggccagccct gacgaatcgc cctgtaagcc
     3121 catttgcgcg tcatttcgaa caggtggcag tttcgcccca tcccgtaacg tttatcgatg
     3181 ctacggcgcg ctgaggcact caaatcgaga taatcagcca gttcaccgag ggtataggct
     3241 tcctctcgcc actccatcac ctgccattcc aggtggaacg gatttttaca aatcagtccg
     3301 ctgtagttca catcagcaca aagtttctca cacagcgaac gttcaatcgc agcggcgtat
     3361 ttcagcgcct taaccgagct gtccggcgcg gttctcacgg cgatattgag cgcgtagagc
     3421 aggtgagcat gaccgttcac aggatttttt acggtgatgt tcggcgcggg agcgttccgg
     3481 tcactccagt caatcgctgc cccctcgcgg tcaacgtcaa aaaccagcca gaactgcgca
     3541 tgaggctggt tctgctggat gtaccgcgca agaagcgcac gccctttgcc ggagatgcga
     3601 agaccaaaag cgagatcatc cgtgtgatag ggacggtggg gtaaattttc gtcgaagtat
     3661 tgaagcgcgg cgctcatgtg ttacgattct ctccgggtta tgcgacctac acgccctctg
     3721 tatttcatgt tgaatcttgc cggatctgga catgatttca ggggcgtttg tttttcagtc
     3781 gcttacacta cacccaacgg caaatcctgt cgatccttcc cgcaaacccg atccatttac
     3841 cataattcct gttgtacgta ccccttaaaa cgctctcaga cgcgttctga cgcaaaaaac
     3901 gcaaaaagac aaggaaacac caggctgaac cagaaaaatg cgtcagaatc gtttttaggg
     3961 cgctgcataa ctatgcacga aagcgatacg cgatcggttt cgaccgttcc gaatggcgaa
     4021 caggtcaaaa tgacaagttt tctcccaccg gatggaaggt tctgcacagg gattttagat
     4081 cccatctccc acatggaact tttgtgacat ttcaaaaaaa agcgcatcag aatcagcctg
     4141 acacgctttg tttttgtcaa agaggatttc gggttactgc gttgctaatc cggtcacttc
     4201 agctgttgat gacggagata cggtcccgcc ctgaacatcc cggatatagt ttgcctgaca
     4261 tgcagccaga ataatgactg caagaagcaa taaaataatc cctgttattt ttttcataac
     4321 gactccttgt tgtgaaagaa tgccagtcat tcatccttaa acaactggca ttcatatttc
     4381 agccctgttt aaatcctgac ttaccgttag cagctcgcca ttctttaatc tctttaataa
     4441 cagcttcagg gctaccatca ttattacctt ctgggtaata aatcagatca gaaccactag
     4501 gatgctcagt aacacttata aaatgctcga ggttatcatc ctgttttttt tcatcacctt
     4561 cacaattgat gatgtcttca ataatttttt tgaattcagt ttcagtgtaa tcactaatgc
     4621 tgtttttcag ttccattttt actgacctct gtgaatatca atatggcgtt tcggcgtagt
     4681 gatacggagg ttgtccatat cataaacacc accatcctga ctgattggct tgtcgtgatg
     4741 aagctcaaat gagcgacgcc caccgacagt atctttattc ctagcccgtg gagctaatcc
     4801 ttggctcaag cgttttttat tacctggatt aaattgctta cttaactcag gatctttcga
     4861 cacttcttcc cagaattttc tccgaaaatc gtcgaagttt ttaaattctt tatcacgcaa
     4921 cttatcagca atgcgatctg gaattggcgc tcctgaatct ttacctgcat catccagcca
     4981 tttatcacca actggtttac ctttacctgt cgccttccct ggcttattcc gtttactctc
     5041 cttatctaat ttatccttag cgtccttttc tttattttcc ttctgtttgc ggcgctcctg
     5101 cgcggcactt aatgcagcat cagcatctga cttctctttt gcagcagcat caaatgcagc
     5161 ctgcttatta tttacatccg tctgcgcccg ctgagcttta agcccggcca tttgccacat
     5221 tctgtgaccg ccagccattg ggtcatgggc aaatcgatta aattgtttta tttcagctat
     5281 tgcatcagca agagttttat tcgctgcatc aagttcgctt ttacgcgaat tataaacctg
     5341 aacagcttta gcctgtcgct cctgatttct ggcaacatct tcatttgcct gattcagctc
     5401 tgcacgcgcg cgttcataat ttcgctcagc cgcttcaacc ggatgcgtag catcccattc
     5461 ctgctgacgg cgattttctt catcctgacg ttgttttacc tggtcaggac taagaacatc
     5521 actcactgaa acatatacgg cattatgacc gctgtccttc gggaatcgaa taactgcatc
     5581 cctggtatta ccaccctgag taaatcctgc cgggcgaaca tccttatcag tattatttgt
     5641 aacacctggg cttaatgtct gtactgctgg cgtactgtta ttaactgaaa tattcagaac
     5701 aggtgcacct ggaattgatg ccgtaaaaac acctggacgt tcggtaggtt ttgcatcaac
     5761 caccggaaca ctcatcggaa cacctgaaac aaccgaaata ttctgtcgtt cgtcttttac
     5821 atcatcaaca acacgaacat ttacgtttac tgttgcctta tcgagaggta atgaactgac
     5881 aggtgattca gtaatatcat ctgcgggtaa tgacgtcaca atctttgaca tcatattggg
     5941 gtcatctttc gctatttccg acggaattaa tgaagataag acaaccccaa aaggagtgaa
     6001 tttaagattt acttttttta atttagcaat aataccagca atagctgccg ataattcgct
     6061 tgcagaaata ctgacagcca gaccgccagc tcctggagtg gaaagcgccg gaaaaccaaa
     6121 tgccactggc gcagctactg ctgacaaatt accgcctgtt cccgagccac caccggaatt
     6181 gccattaccc ccgccattac cacgaccgga gccacctccc cagtgaatgc cgctaccgga
     6241 accaccaccc cacgggttat tttccgaact ccatcctgaa ccatcagaag caccaccact
     6301 tacaccaatc ccggtcgggc caccattaat gttaccactt gtgctatgcg cgcccgtgtt
     6361 atggccgcgt ccatctccac cgctcataaa agtccctctt ttaagcgtta aaacaattaa
     6421 ttacatgatt aatactgtac ataaaaccac tggttttatg tacagatcaa aaagtacccg
     6481 tcatcgtttt ttctgtcaag ttcaggtgga aaatttttta gaagtcagga gggataacac
     6541 ttagaaaacg cagagcgggc cacaaaaaat tttgtggccc gtgatgtgtt ggcggggtcc
     6601 ggagtgcagc cccggacatc acatactcat gccccggaag tgcccgggac ggcgttgttg
     6661 ttgcctgcgc tgttcctccc gcttccactc atgccaggtc tgattaaatc cgagttgacg
     6721 gaagagataa tccggcatct cttccgg
//
