LOCUS       NZ_CP027200.1          10672 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  NZ_CP027200.1 Escherichia coli strain WCHEC025943 plasmid p2_025943,
            complete sequence.
ACCESSION   NZ_CP027200
VERSION     NZ_CP027200.1
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##antiSMASH-Data-START##
            Version      :: 7.1.0
            Run date     :: 2024-06-07 21:06:56
            NOTE: This is a single region extracted from a larger record!
            Orig. start  :: 58197
            Orig. end    :: 68869
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..10672
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="True"
                     /core_location="[5000:5672]"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5672
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     cand_cluster    1..10672
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     region          1..10672
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="RiPP-like"
                     /region_number="1"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     CDS             417..1688
                     /locus_tag="ctg1_64"
                     /transl_table=11
                     /translation="MFALADINSFYASCEKVFRPDLRNEPVIVLSNNDGCVIARSPEAK
                     ALGIRMGQPWFQVRQMRLEKKIHVFSSNYALYHSMSQRVMAVLESLSPAVEPYSIDEMF
                     IDLRGINHCISPEFFGHQLREQVKSWTGLTMGVGIAPTKTLAKSAQWATKQWPQFSGVV
                     ALTAENRNRILKLLGLQPVGEVWGVGRRLTEKLNALGINTALQLAQANTAFIRKNFSVI
                     LERTVRELNGESCISLEEAPPAKQQIVCSRSFGERITDKDAMHQAVVQYAERAAEKLRG
                     ERQYCRQVTTFVRTSPFAVKEPCYSNASVEKLPLPTQDSRDIIAAACRALNHVWREGYR
                     YMKAGVMLADFTPSGIAQPGLFDEIQPRKNSEKLMKTLDELNQSGKGKVWFAGRGTAPE
                     WQMKREMLSPSYTTQWTEIPIASF"
     CDS             complement(1693..2085)
                     /locus_tag="ctg1_65"
                     /transl_table=11
                     /translation="MDDERKRKKFTLYLHPEKAADFQTLEAIESVPRSERGELFRNAFI
                     SGMALHQLDPRLPVLLTAILSEEFSADQVVTLLSQTTGWKPSQADIRAVLTELGALQSA
                     EKMPPSATDSVQEAMNDVRLKMQKLF"
     CDS             complement(2090..3061)
                     /locus_tag="ctg1_66"
                     /transl_table=11
                     /translation="MNVYCDDGSTTIKLAWNDNGKICKSLSQNSFRHGWKVDGLGIRQT
                     FNYELDGKKYTYDEVSNQSILTTHIEYQYTDVNLLAVHHALLNSGLVPQPVSLTVTLPI
                     SEFYTKECQKNELNIQRKIENLMRPIRLNKGDVFTIEHVDVMPESLPAVFSRLVMDKVG
                     QFEKSLVVDIGGTTLDVGVIVGQFDSVSAIHGNSGIGVSSVTKAAMSALRMASSDTSFL
                     VADELIKRRNDPDFVRQVINDETKTDLVLNTIEGAIASLGEQVVNELGDFHHVNRVYVV
                     GGGAPLIYDSIKTAWHHLGQKVVMMESPQTALVEAIAAFKEE"
     CDS             3290..3934
                     /gene_functions="other (smcogs) SMCOG1157:hypothetical
                     protein (Score: 48.4; E-value: 1.5e-14)"
                     /gene_kind="other"
                     /locus_tag="ctg1_67"
                     /transl_table=11
                     /translation="MITVVGGNKGGSGKTTIASNLAIALANKGREVCLLNGDLQRTAAK
                     HHAEREAAGLLPAITLVEKFDNLTQTLQALNEKFDDVIVDVAGRNSKEFITSGVVAHQI
                     IAPLQCSQPDLDTLTELEQQIDAMRNLNPELKVYCLQSMATTNPVLRGNERKEFLEYLE
                     EFPTIQVLDSVICFRKVYRDCMSNGTGVVETNNTAARAEIEHLMNEVFGPW"
     CDS             3928..4203
                     /locus_tag="ctg1_68"
                     /transl_table=11
                     /translation="MVKKPSQQALNRAAVTVEQAEALAQRLADKPYGAPEKPEPEKQCR
                     TTISLGESMLVTIEDLALRNKRNGKDPKNVSAIVRVALEQYLKTLT"
     CDS             complement(4465..4773)
                     /locus_tag="ctg1_69"
                     /transl_table=11
                     /translation="MPAGQQTHRLVRLSDSWYVSQLQTMVATLKIPMERRNKRTGRTEK
                     ARIWEVTDRKVRTWIGEAVAAADGVTFSVPVTPHTFRHSYAMHMLYAAEGPAEPDGA"
     CDS             5001..5672
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: Cloacin"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg1_70"
                     /sec_met_domain="Cloacin (E-value: 3.4e-52, bitscore:
                     169.6, seeds: 4, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSDNEGSVPTEGIDYGDTMVVWPSTGRIPGGDVKPGGSSGLAPSM
                     PPGWGDYSPQGIALVQSVLFPGIIRRIILDKELEEGDWSGWSVSVHSPWGNEKVSAART
                     VLENGLRGGLPEPSRPAAVSFARLEPASGNEQKIIRLMVTQQLEQVTDIPASQLPAAGN
                     NVPVKYRLTDLMQNGTQYMAIIGGIPMTVPVVDAGNDSNLLIVFYVQIMPDDFVMQLHR
                     F"
     CDS             complement(5607..5954)
                     /locus_tag="ctg1_71"
                     /transl_table=11
                     /translation="MSRQRWLFYAYDSLRKTVVAHVFGERTMATLGRLMSLLSPFDVVI
                     WMTDGWPLYESRLKGKLHVISKRYTQRIERHNLNLRQHLARLGRKSLSFSKSVELHDKV
                     IGHYLNIKHYQ"
     CDS             complement(6018..6293)
                     /locus_tag="ctg1_72"
                     /transl_table=11
                     /translation="MASVSISCPSCSATDGVVRNGKSTAGHQRYLCSHCRKTWQLQFTY
                     TASQPGTHQKIIDMAMNGVGCRATARIMGVGLNTILRHLKNSGRSR"
     CDS             6635..7924
                     /gene_functions="transport (smcogs) SMCOG1106:major
                     facilitator transporter (Score: 334.7; E-value: 1e-101)"
                     /gene_kind="transport"
                     /locus_tag="ctg1_73"
                     /transl_table=11
                     /translation="MKRKTMGWLIVFLLFIVYMLNYMDRSALSITAPLIEKELGFNAAE
                     MGMIFSAFFVGYALFNFIGGWASDKVGPKTVFLVAALLWSVFCGMTGLVTGLWTMLAVR
                     VLFGMAEGPVSAAGNKIINNWISRKESATAIGIFSAGSPLGGAISGPVVGLLAISLGWR
                     PAFGIIFIFGLVWVLLWYFFVSDKPSKSKRLAAEERVDFENHDDIILAEDGKGTPSLGY
                     YMKQPMVWATTLAFFSYNYILFFFLTWFPSYLNHSLHLDIKEISIATVIPWVIGAIGMV
                     LGGVCSDFIYRITGNALLSRRLILGICLAGAAVCVAVSGTVTTIGSAVTLMSVSLFLLY
                     LTGPIYWAVIQDVVHKDKVGSVGGAMHGLANISGIIGPLVTGFIVQFSGKYDYAFYLAG
                     AIAIVSSLLVFVFVKNKGLKVNESQSCVHQ"
     CDS             7896..8921
                     /NRPS_PKS="Domain: PKS_ER (23-308). E-value: 1.2e-12.
                     Score: 39.4. Matches aSDomain:
                     nrpspksdomains_ctg1_74_PKS_ER.1"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ADH_N"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ADH_zinc_N"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1040:alcohol dehydrogenase (Score: 293.1; E-value:
                     3.9e-89)"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg1_74"
                     /sec_met_domain="ADH_N (E-value: 3.1e-33, bitscore: 106.5,
                     seeds: 66, tool: rule-based-clusters)"
                     /sec_met_domain="ADH_zinc_N (E-value: 9.8e-26, bitscore:
                     82.8, seeds: 86, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKVKAVYINKPGEIATRLVDYPVKKDNEVLIKVDAAGICGSDIGA
                     FRGTNPLVTYPRIIGHEVTGIVLETGEGMPEDIKQGDRVIVDPYIYCGHCYPCSVGRTN
                     CCENLKVIGVHIDGAMQEVVAHPAHLIHKIPDNVPSEMAPLAEPLTIALHALHRAQLKA
                     GEHVVIIGAGAIGLMAALSALRYHATPILVDIVDERLQYAQSLGVPYVVNSAKANVVEA
                     VKDITQGTMAQVVVEASGANSAIRSTLDLASFAGRIAYTGWPKQETSLPTNLITFKELD
                     LRGSRTSAGEFEEALQMLSTLNINPADIVSKVVTIDEVPEAVKELDQFPERHLKINVKF
                     I"
     aSDomain        7965..8819
                     /aSDomain="PKS_ER"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_ctg1_74_PKS_ER.1"
                     /evalue="1.20E-12"
                     /label="ctg1_74_PKS_ER.1"
                     /locus_tag="ctg1_74"
                     /protein_end="308"
                     /protein_start="23"
                     /score="39.4"
                     /tool="antismash"
                     /translation="KKDNEVLIKVDAAGICGSDIGAFRGTNPLVTYPRIIGHEVTGIVL
                     ETGEGMPEDIKQGDRVIVDPYIYCGHCYPCSVGRTNCCENLKVIGVHIDGAMQEVVAHP
                     AHLIHKIPDNVPSEMAPLAEPLTIALHALHRAQLKAGEHVVIIGAGAIGLMAALSALRY
                     HATPILVDIVDERLQYAQSLGVPYVVNSAKANVVEAVKDITQGTMAQVVVEASGANSAI
                     RSTLDLASFAGRIAYTGWPKQETSLPTNLITFKELDLRGSRTSAGEFEEALQMLSTLNI
                     NPAD"
     CDS             complement(8980..9651)
                     /gene_functions="regulatory (smcogs) SMCOG1071:GntR family
                     transcriptional regulator (Score: 138.3; E-value: 5.4e-42)"
                     /gene_kind="regulatory"
                     /locus_tag="ctg1_75"
                     /transl_table=11
                     /translation="MLIPEIKQERLYQKIANLIIKLINDNVFPPGSFLPPERELAKQLG
                     VSRSSLREALIVLEISGWIIIQSGNGVIVSDKKPPHVDYTIEEILYTRDLVDSHCARLA
                     ALANNETIINKIEALYLRMENAINENNVAEFYTLDKQFHLAISEASHNRVLLDMSRMLW
                     EQRINIPYAGLDKRSGDKNVLINLNQQHKAIIEAIRAGDADNALQQALLHLKYVREIIG
                     Y"
     CDS             complement(9746..10063)
                     /locus_tag="ctg1_76"
                     /transl_table=11
                     /translation="MIYEHIRSSDYVFRYGGEEFMILLVETELSKAKIILENLRNKMAD
                     LRIQASASESFSVTVSIGVAEYDYHPDYKLLIEKADSALYYAKSQGRNRVEVFQPHMTN
                     G"
     CDS             complement(10078..10272)
                     /locus_tag="ctg1_77"
                     /transl_table=11
                     /translation="MFEDLVKEENGKDSLTQLLNRRFIPTIMRREISIALHSRKPFALV
                     LITSSKLMIVMGIIPGIIR"
ORIGIN
        1 ccggctgacc cttcaggcga tgacagttac gtgcgaccgt tgtttgccga tcgttgccag
       61 gccggttttc cttcgcctgc cactgattat gctgagcagg aactggatct gaacagctat
      121 tgcatcagca gacctgcagc caccttcttt ctgcgcgcca gcggtgaatc gatgaaccag
      181 gctggcgtgc agaatggtga tctgctggta gtggacaggg ccgaaaaacc acaacacggg
      241 gacatcgtta tcgctgagat cgacggtgag ttcaccgtca aacgactgct gttgcgccca
      301 cgcccggcac tggagccggt ttcagacagc ccggagttcc gcacactgta tccggaaaac
      361 atctgtattt ttggtgttgt cactcacgtg atacacagga cgcgggagtt acgctgatgt
      421 ttgcactggc tgatatcaac agtttctacg cctcatgtga aaaagttttc cgcccggacc
      481 ttcgcaacga accagtcatc gtactcagca ataacgatgg ctgtgtgatc gcgcgcagcc
      541 cggaggcaaa agcccttggc atcagaatgg ggcagccctg gtttcaggtg agacagatgc
      601 gcctggagaa gaaaatacat gtattttcca gcaattatgc gctgtaccac agcatgagcc
      661 aacgggttat ggctgttctg gagtcgcttt ctcccgcagt tgagccctac tcaattgatg
      721 aaatgttcat tgatttgcgg gggataaatc attgcatctc tccggagttt tttggtcatc
      781 agctcaggga acaggtaaaa agctggacag gactcaccat gggggtgggc attgcgccta
      841 caaaaacgct ggctaaaagt gcacagtggg caacaaagca atggccacag ttttccggtg
      901 tggtcgcgct gacggcagaa aaccgtaatc ggatcttgaa gctactgggg ctgcagccag
      961 ttggtgaggt ctggggagta ggacgcagac tgacggaaaa gctgaatgcg ctgggtatta
     1021 acacagcact gcagctggcg caggctaaca cggcattcat ccggaaaaac ttcagcgtca
     1081 ttcttgagcg tacggtacgc gaactcaacg gcgagtcctg catatccctg gaagaagcac
     1141 caccggcaaa acagcagatt gtctgtagtc gcagttttgg tgaacgaatc acagacaaag
     1201 atgccatgca ccaggctgtt gttcagtatg cagagcgggc cgcagagaaa ctacgtgggg
     1261 agcgtcagta ttgccggcag gtgacgacat ttgtacggac atcccccttt gcagtaaaag
     1321 aaccctgtta cagcaatgcc tctgtggaaa agcttccatt acccacacag gacagccggg
     1381 acattattgc cgcagcatgc agagcgttaa accatgtctg gcgtgaaggg taccgctata
     1441 tgaaggcagg agtcatgctg gctgatttca caccatcggg tatagcgcag ccgggattat
     1501 ttgatgagat ccagccccgt aaaaacagcg aaaagttaat gaaaacactc gatgaactga
     1561 accagtctgg aaaagggaaa gtgtggtttg cggggcgagg aaccgcccct gaatggcaaa
     1621 tgaaacggga aatgctctcg ccttcgtata caacacaatg gactgagatc cccattgctt
     1681 cattctgata tatcagaata atttttgcat tttaaggcgc acatcattca ttgcctcctg
     1741 caccgaatca gtagcagaag gaggcatttt ttcagcagat tgcaaagcac cgagttcagt
     1801 caacacagct ctgatatcgg cctgagaagg cttccatcct gtcgtctgac tgagtaaggt
     1861 tacaacctga tctgctgaaa actcctcgct caaaatggca gtcagcaata caggcaggcg
     1921 aggatcaagt tggtgtaacg ccattccaga aataaatgca ttccggaaca gctcaccacg
     1981 ctcagaacgc ggaacggact ctatcgcttc caaagtttga aagtctgcag ctttttccgg
     2041 atgcagataa agtgtgaatt ttttcctttt ccgttcatcg tccacaatat tactcctctt
     2101 tgaaagccgc gatagcttca accagtgctg tctgtggcga ctccatcatc actacttttt
     2161 gcccaagatg gtgccatgcc gtttttatcg aatcatagat caacggggcc ccaccaccga
     2221 caacataaac acgattcaca tgatgaaaat caccgagctc attgactact tgctccccca
     2281 gactggcaat agccccctca atggtattaa ggaccagatc agtttttgtt tcgtcattta
     2341 taacctgccg aacaaagtcc gggtcattac gacgtttaat caattcatct gcaacgagga
     2401 aactggtatc actggatgcc atgcgcaatg cactcattgc agctttcgta actgaagaca
     2461 caccaatgcc agaattacca tgaatggcac ttacagagtc aaattgacca acgataacac
     2521 caacatcaag cgtagtgccg ccaatatcaa ctaccagcga tttttcgaac tgaccaactt
     2581 tatccatgac cagacgagaa aacacagctg gcaatgattc aggcatcaca tcaacgtgtt
     2641 caatggtgaa aacatcgcct ttattaagac gaatcggcct cattaaattc tcaattttac
     2701 gctgaatgtt gagttcattt ttttgacact ctttcgtgta gaactcactg ataggcaaag
     2761 tcacagtcaa tgatacaggc tgaggcacta atccactatt cagtaaagcg tgatgcacag
     2821 ctaacaggtt aacatccgta tattgatact caatatgagt cgtaagaata gattggttgc
     2881 taacttcatc atacgtgtat tttttaccat ccagttcata gttaaatgtc tggcgaatcc
     2941 ctaacccgtc aaccttccat ccatgacgaa atgagttttg cgacaacgac ttacagattt
     3001 tcccattgtc attccatgcc aacttgattg ttgttgaacc atcatcgcag tatacgttca
     3061 tctatagccc cttaaatact caatctgagt aaatcactgt ttctggttgc aaggtaatac
     3121 tcaatttgag cactatcata gttatgaaat cacaaaacac aagtaatatt tattacatca
     3181 tatgacatac ttcatatttc atacttcata aatatgaatg catgttgaca catcaggtta
     3241 ccgtgtcaaa atatcatcat acttcacaca tcacatttat gaggttgcaa tgattactgt
     3301 agttggtggg aacaaaggtg gttcagggaa aactaccatt gcatcaaacc tggctattgc
     3361 tctcgcaaat aaaggaaggg aagtctgcct gttaaacgga gatctacaac gaacagccgc
     3421 aaaacatcat gcggaacgag aagctgcagg actgttacca gcaataaccc ttgttgagaa
     3481 atttgataac ctgacacaaa cactacaggc tctgaatgaa aagttcgatg atgtaatagt
     3541 ggatgtcgca ggaagaaact caaaggaatt cataacgagt ggagttgtcg cacatcaaat
     3601 aattgcgcca ctacagtgtt ctcaaccaga tctggatact cttacagaac tagaacaaca
     3661 gatagacgca atgcgtaacc ttaacccgga acttaaggtt tattgtttac aaagtatggc
     3721 aacaaccaat ccggttttga ggggaaatga gagaaaagaa ttccttgaat atcttgaaga
     3781 attcccaaca attcaagtcc tggattcagt tatttgtttc agaaaagtct atcgcgattg
     3841 catgagcaac ggcaccggtg tagtagaaac gaacaataca gcagccagag cagaaattga
     3901 acatttaatg aatgaggtat ttggcccatg gtgaagaaac ccagccagca agcattaaac
     3961 agagctgcag ttacggtaga acaagcagag gctctggctc agcgccttgc ggataaacca
     4021 tacggcgcgc cggaaaaacc cgaaccagaa aaacaatgtc ggacgacaat ctctcttggt
     4081 gaaagcatgc ttgtcacaat agaagatctg gcattgagga ataaacgcaa tggtaaagat
     4141 ccaaaaaatg taagtgccat cgtcagagta gcccttgagc aatacttaaa aacactaacg
     4201 taataatcaa gaagttatta cttatgaaat cacacttgtg aattcacact gtttaagcac
     4261 ccttgcatac aagagatcac atattgcagt ggtctcttct tttatagaat aaatcacaat
     4321 tcataattat gatttatgaa ttatgcgtgt ctgtttttca gcattgtgac cgcatcggac
     4381 tcaggcatcg aaaactacac ccggtgccgt gcagccacat ccagcgcaaa caccttcgtg
     4441 tagacctccg ttgagctgat ggacttatgc cccatcaggc tctgcaggac cttcagcggc
     4501 atacagcatg tgcatcgcat aggaatggcg gaacgtatgt ggcgtgaccg ggacagagaa
     4561 cgtcacacca tcagcggcag caaccgcttc cccaatccag gtcctgacct ttctgtccgt
     4621 cacttcccag atccgcgctt tctctgtcct gcctgttcgt ttattacgcc gttccatggg
     4681 gattttcagt gtggccacca tcgtctgcag ctggctgacg taccaggagt ccgagagccg
     4741 aaccagtcgg tgagtctgct gaccggcggg cattattcct gccgtcctga ccagtaatgc
     4801 ttgtgcagtt ttttgtggca tcaggcgaga atagcgcgtg gtgtgagtgc ctgttttttg
     4861 atcgttttca caaaaattga aatccacagt cttgacagag aaaacacagc ggcgtaattt
     4921 ttgatctgta cataaaacca gtggttatat gtacagcatt taattttaat tgattgtttt
     4981 taaagtcaaa gaggttttct atgagtgata atgaaggtag tgtaccgaca gaaggtattg
     5041 attacgggga cacaatggtt gtgtggccgt caacaggacg aattccgggc ggtgatgtga
     5101 aacccggagg ctcatcaggt ctcgctccat ccatgcctcc gggatggggg gattacagcc
     5161 cacaaggtat cgcacttgta caaagtgttc tttttcctgg aattattcgc cggattattc
     5221 ttgataagga acttgaagag ggagactggt cgggatggtc tgtcagtgtg catagcccct
     5281 ggggaaacga gaaagtttcc gctgcacgaa cagttcttga aaatggttta cgtggtggtt
     5341 tgccagaacc gtctcgcccg gctgctgttt cttttgcccg tctggagcct gcttccggaa
     5401 atgagcaaaa aattattcgt cttatggtta cacagcaact ggagcaggta acggatatcc
     5461 ctgccagcca gttaccagca gcgggtaata atgtaccggt aaaatatcgt ctgacggacc
     5521 ttatgcagaa tggtacacaa tatatggcta ttatcggagg tattccgatg acagtgccag
     5581 tagtggatgc cggtaatgac tccaacttat tgatagtgtt ttatgttcag ataatgcccg
     5641 atgactttgt catgcagctc caccgatttt gagaacgaca gcgacttccg tcccagccgt
     5701 gccaggtgct gcctcagatt caggttatgc cgctcaattc gctgcgtata tcgcttgctg
     5761 attacgtgca gctttccctt caggcgggat tcatacagcg gccagccatc cgtcatccat
     5821 atcaccacgt caaagggtga cagcaggctc ataagacgcc ccagcgtcgc catagtgcgt
     5881 tcaccgaata cgtgcgcaac aaccgtcttc cggagactgt catacgcgta aaacagccag
     5941 cgctggcgcg acatagcccc actgttcgtc catttccgcg cagacgatga cgtcactgcc
     6001 cggctgtatg cgcgaggtta ccgactgcgg cctgagtttt ttaagtgacg taaaatcgtg
     6061 ttgaggccaa cgcccataat gcgggctgtt gcccggcatc caacgccatt catggccata
     6121 tcaatgattt tctggtgcgt accgggttga gaagcggtgt aagtgaactg cagttgccat
     6181 gttttacggc agtgagagca gagatagcgc tgatgtccgg cggtgctttt gccgttacgc
     6241 accaccccgt cagtagctga acaggaggga cagctgatag aaacagaagc cactggagca
     6301 cctcaaaaac accatcatac actaaatcag taagttggca gcatcaccta tttttatgcg
     6361 taaaataaat ccttactgca ttaaatactg catcttttag tttccggatg aaaataatcc
     6421 atattgttta tttcctgagt gtgatattaa ccactcaaaa cagagtaaat aaaatcaaca
     6481 aaatcatgca attagagcgc gattgtaatt ttcattgaat actggttatc caattttcgt
     6541 tatattgatc acaatatcct tcttttcatg atgatctaaa aataaatatt aaatattgtg
     6601 gtcaggccgc tagaccacag caagaggaaa tatcatgaaa agaaaaacaa tgggatggct
     6661 tatcgttttt cttctgttta tagtttacat gctgaactat atggatcgtt ctgcattatc
     6721 gattacggcc ccacttattg aaaaagagtt aggctttaat gctgctgaaa tggggatgat
     6781 tttcagcgca tttttcgttg gttatgcttt atttaacttc attggcggtt gggccagtga
     6841 caaagttgga ccgaaaacag tatttttggt tgctgcactg ttatggtctg ttttttgtgg
     6901 tatgaccggc ctggtcaccg ggttatggac gatgcttgcc gtacgcgttt tatttggtat
     6961 ggcagaaggc ccggttagtg cagcaggcaa taaaattatt aataactgga tctcgcgtaa
     7021 agagtcggca acagccatcg gtatttttag tgctggttca ccacttggcg gtgctatttc
     7081 tggtccggtt gttggcttat tggcgatatc acttggctgg cgtccggcat ttggcatcat
     7141 ctttatattt ggcctggttt gggtattact gtggtacttc tttgtcagtg ataaaccgtc
     7201 aaaaagtaaa cgtctggcag ctgaagagcg tgtcgatttt gaaaatcatg acgatattat
     7261 tctggccgaa gatggtaaag gtacgccttc gctgggttat tacatgaaac agccgatggt
     7321 atgggcgact actctggcat tctttagcta caactatatt ctgtttttct ttcttacctg
     7381 gttcccaagc tatttgaacc attcgttaca ccttgatatt aaagaaatta gtattgcaac
     7441 tgttatcccg tgggttattg gtgcaatcgg catggtgcta ggtggcgttt gctctgactt
     7501 tatttatcgc attacgggaa atgccttact ttctcgccgc ttaattctcg gtatttgcct
     7561 tgcgggggcc gcagtctgtg tcgcagtttc cggaacggtt accactattg gtagtgcagt
     7621 tacgctgatg tcggtttcct tattcctgct ttatttaact ggacctattt attgggctgt
     7681 tattcaggat gtggtgcata aagataaagt aggtagcgta ggcggtgcga tgcacgggct
     7741 ggcaaatatt tccggcatta ttggtccatt ggtaaccggt tttattgttc agttcagcgg
     7801 taaatatgat tacgcattct atctggcagg tgccatagca attgtttcaa gtctgctggt
     7861 attcgtattt gttaaaaata aaggattgaa agtcaatgaa agtcaaagct gtgtacatca
     7921 ataaacctgg tgaaattgcc actcgcctgg tcgattatcc ggtaaaaaaa gataatgaag
     7981 ttttaattaa agtggatgca gcggggattt gtggttctga catcggtgca tttcgtggca
     8041 ctaacccact ggtaacttat ccgcgaatta ttggacatga agtcaccggg attgttctgg
     8101 aaactggtga ggggatgcca gaagatatta agcagggcga ccgggttatt gtagatccct
     8161 atatttattg cggtcactgt tatccatgtt ctgtcggcag aactaattgc tgtgagaact
     8221 taaaagtcat cggcgtacat attgatggcg ctatgcaaga agtggttgcg catccggctc
     8281 acttaattca caaaattcct gacaatgtac cgtcagaaat ggcgccactg gctgaaccct
     8341 taactattgc cctgcacgca ttacatcggg cgcaattgaa agcaggtgaa catgtcgtca
     8401 ttattggtgc tggtgccatt ggacttatgg cggcactgag tgcgttgcgc tatcacgcaa
     8461 cccccattct ggttgatatt gtcgatgaac ggttgcaata cgcacaatcg ctcggtgtgc
     8521 cttacgtagt taactccgca aaagctaacg ttgttgaggc agtgaaagat attacacaag
     8581 ggacgatggc tcaggttgtg gtggaagcct ctggtgcaaa tagcgcgatc agaagtaccc
     8641 tggatctggc ctcatttgcc gggcgtattg cctatacggg ttggccaaaa caggaaacat
     8701 cgttaccaac taatttgatc acctttaaag aactggattt acgcggttcc cgtaccagtg
     8761 ccggtgaatt tgaagaagct ctgcaaatgt tatcaacgct gaatattaat ccggcagata
     8821 tcgtgagtaa agtcgtgaca attgatgaag ttcctgaggc tgttaaagag ttagatcaat
     8881 tcccggaacg ccatctgaaa attaacgtga aattcattta attagcaaaa atcaaaacgc
     8941 gtagtgaatt cactacgcgt ttttttagcg cagaaaaggt taataaccaa ttatttctcg
     9001 cacatacttc aaatgtagca gtgcctgttg caaggcattg tctgcatctc ctgcacgaat
     9061 cgcctcaatg attgctttat gctgttgatt aaggttgata agtacattct tatcgccgga
     9121 acgtttatcc agcccggcat agggtatatt gatgcgctgc tcccaaagca tccgtgacat
     9181 atctaataac acccgattat gactggcttc agaaatggcc agatgaaact gcttatcaag
     9241 cgtataaaat tcagccacat tattttcgtt aatggcattt tccattcgta aataaagtgc
     9301 ctcaatttta ttaatgattg tttcattatt agccagtgct gcaagtcggg cacaatgact
     9361 atcaactaaa tcacgggtat aaagaatttc ttcgatagtg taatcgacat gagggggttt
     9421 tttatcgctg acaatcaccc catttccaga ctgaataata atccagccag atatctccag
     9481 aacaataagc gcttctctta gcgatgaacg actaaccccc agttgtttcg ccagttcacg
     9541 ttctggaggt aaaaagctac ctggtggaaa cacattatca ttgatgagct taataataag
     9601 attcgctatc ttttgataga gcctttcctg tttaatttct ggaattagca tgtgcctccc
     9661 gctcgatttt atcgttagtt atccgacgaa ttatcatacc atccctggtt cggatctact
     9721 gctttcgagt gacaatgacg aaaaatcagc cgtttgtcat atgtggctgg aaaacttcta
     9781 cccggtttcg gccctgactt ttggcgtaat aaagtgcact atctgccttt tctatcaata
     9841 gcttatagtc aggatgataa tcatattcgg cgacgccaat actgaccgtg accgaaaaac
     9901 tttcactggc tgaagcctgg atacgcaaat cagccatttt gttccgtaga ttttcgagaa
     9961 taattttggc ttttgataat tctgtttcga caagcaaaat cataaattct tcgccaccat
    10021 agcggaaaac ataatcactg ctgcgaatat gttcataaat catcgcagag acatttttca
    10081 gcgtattatc cccggtatta tgcccataac gatcattaat ttgcttgaag taatcaatac
    10141 gagggcgaac ggtttacgtg agtgcagggc gatagagatt tcccgccgca taatcgtcgg
    10201 aataaagcgc cgatttaata gttgtgtcag agaatcttta ccgttttctt cttttaccag
    10261 atcttcgaac atggaagagg gtaatgactc caacttattg atagtgtttt atgttcagat
    10321 aatgcccgat gactttgtca tgcagctcca ccgattttga gaacgacagc gacttccgtc
    10381 ccagccgtgc caggtgctgc ctcagattca ggttatgccg ctcaattcgc tgcgtatatc
    10441 gcttgctgat tacgtgcagc tttcccttca ggcgggattc atacagcggc cagccatccg
    10501 tcatccatat caccacgtca aagggtgaca gcaggctcat aagacgcccc agcgtcgcca
    10561 tagtgcgttc accgaatacg tgcgcaacaa ccgtcttccg gagcctgtca tacgcgtaaa
    10621 acagccagcg ctggcgcgat ttagccccga cgtatcccca ctgttcgtcc at
//
