NZ_CP026396.1 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

NZ_CP026396.1

Region	Type	From	To	Most similar known cluster		Similarity
Region 1	NRPS,T1PKS	1	41,470

NZ_CP026396.1 - Region 1 - NRPS,T1PKS

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Location: 1 - 41,470 nt. (total: 41,470 nt) Show pHMM detection rules used

Region on contig edge.

NRPS: cds(Condensation and (AMP-binding or A-OX))
T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

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Gene overview

NRPS/PKS domains

NRPS/PKS modules

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Detailed domain annotation

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NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 0 - 41470:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (mal - Cys) + (Ala - Cys) + (Asp)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

ctg1_5

Search NORINE for peptide: strict or relaxed

AMP-binding (446..850): Asp

nrpys: Asp

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: asp,asn (Asp, Asn)
Single AA prediction:: asp (Asp)

Stachelhaus prediction details:

Stachelhaus sequence:: DLTKLGHVGK
Nearest Stachelhaus code(s):: Asp DLTKIGHVGK (71% 8Å match); OH-Asp DLTKVGHVGK (68% 8Å match); Asn DLTKLGEVGK (53% 8Å match)
Stachelhaus code match:: 90% (moderate)

ctg1_9

Search NORINE for peptide: strict or relaxed

AMP-binding (22..407): Ala

nrpys: Ala

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Tiny, hydrophilic, transition to aliphatic (Gly, Ala)
Single AA prediction:: ala (Ala)

Stachelhaus prediction details:

Stachelhaus sequence:: DLFNNALTYK
Nearest Stachelhaus code(s):: Ala DLFNNALTYK (91% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (1056..1462): Cys

nrpys: Cys

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Polar, uncharged (aliphatic with -SH) (Cys)
Small clusters prediction:: Polar, uncharged (aliphatic with -SH) (Cys)
Single AA prediction:: Polar, uncharged (aliphatic with -SH) (Cys)

Stachelhaus prediction details:

Stachelhaus sequence:: DLYNFSLIWK
Nearest Stachelhaus code(s):: Cys DLYNMSLIWK (79% 8Å match)
Stachelhaus code match:: 90% (moderate)

ctg1_10

Search NORINE for peptide: strict or relaxed

PKS_AT (535..826): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 87.5%; inactive: 83.3%; 2-Rhyd-Malonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 153.1
inactive: 97.7
Methylmalonyl-CoA: 44.8
Methoxymalonyl-CoA: 44.4
Propionyl-CoA: 37.8
Ethylmalonyl-CoA: 26.0
Benzoyl-CoA: 24.7
fatty_acid: 24.4
Isobutyryl-CoA: 24.3
Acetyl-CoA: 22.6
2-Methylbutyryl-CoA: 15.3
CHC-CoA: 14.3
trans-1,2-CPDA: 0.0
3-Methylbutyryl-CoA: 0.0

AMP-binding (1472..1867): Cys

nrpys: Cys

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Polar, uncharged (aliphatic with -SH) (Cys)
Small clusters prediction:: Polar, uncharged (aliphatic with -SH) (Cys)
Single AA prediction:: Polar, uncharged (aliphatic with -SH) (Cys)

Stachelhaus prediction details:

Stachelhaus sequence:: DLYNMSLIWK
Nearest Stachelhaus code(s):: Cys DLYNMSLIWK (94% 8Å match)
Stachelhaus code match:: 100% (strong)