LOCUS       NZ_CP020510.1          10672 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  NZ_CP020510.1 Escherichia coli strain 165 plasmid unnamed1, complete
            sequence.
ACCESSION   NZ_CP020510
VERSION     NZ_CP020510.1
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##antiSMASH-Data-START##
            Version      :: 7.1.0
            Run date     :: 2024-06-07 23:17:47
            NOTE: This is a single region extracted from a larger record!
            Orig. start  :: 40908
            Orig. end    :: 51580
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..10672
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5672]"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5672
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     cand_cluster    1..10672
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     region          1..10672
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="1"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     CDS             401..595
                     /locus_tag="ctg1_47"
                     /transl_table=11
                     /translation="MFEDLVKEENGKDSLTQLLNRRFIPTIMRREISIALHSRKPFALV
                     LITSSKLMIVMGIIPGIIR"
     CDS             610..927
                     /locus_tag="ctg1_48"
                     /transl_table=11
                     /translation="MIYEHIRSSDYVFRYGGEEFMILLVETELSKAKIILENLRNKMAD
                     LRIQASASESFSVTVSIGVAEYDYHPDYKLLIEKADSALYYAKSQGRNRVEVFQPHMTN
                     G"
     CDS             1022..1693
                     /gene_functions="regulatory (smcogs) SMCOG1071:GntR family
                     transcriptional regulator (Score: 138.3; E-value: 5.4e-42)"
                     /gene_kind="regulatory"
                     /locus_tag="ctg1_49"
                     /transl_table=11
                     /translation="MLIPEIKQERLYQKIANLIIKLINDNVFPPGSFLPPERELAKQLG
                     VSRSSLREALIVLEISGWIIIQSGNGVIVSDKKPPHVDYTIEEILYTRDLVDSHCARLA
                     ALANNETIINKIEALYLRMENAINENNVAEFYTLDKQFHLAISEASHNRVLLDMSRMLW
                     EQRINIPYAGLDKRSGDKNVLINLNQQHKAIIEAIRAGDADNALQQALLHLKYVREIIG
                     Y"
     CDS             complement(1752..2777)
                     /NRPS_PKS="Domain: PKS_ER (23-308). E-value: 1.2e-12.
                     Score: 39.4. Matches aSDomain:
                     nrpspksdomains_ctg1_50_PKS_ER.1"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ADH_N"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ADH_zinc_N"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1040:alcohol dehydrogenase (Score: 293.1; E-value:
                     3.9e-89)"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg1_50"
                     /sec_met_domain="ADH_N (E-value: 4.7e-33, bitscore: 106.5,
                     seeds: 66, tool: rule-based-clusters)"
                     /sec_met_domain="ADH_zinc_N (E-value: 1.5e-25, bitscore:
                     82.8, seeds: 86, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKVKAVYINKPGEIATRLVDYPVKKDNEVLIKVDAAGICGSDIGA
                     FRGTNPLVTYPRIIGHEVTGIVLETGEGMPEDIKQGDRVIVDPYIYCGHCYPCSVGRTN
                     CCENLKVIGVHIDGAMQEVVAHPAHLIHKIPDNVPSEMAPLAEPLTIALHALHRAQLKA
                     GEHVVIIGAGAIGLMAALSALRYHATPILVDIVDERLQYAQSLGVPYVVNSAKANVVEA
                     VKDITQGTMAQVVVEASGANSAIRSTLDLASFAGRIAYTGWPKQETSLPTNLITFKELD
                     LRGSRTSAGEFEEALQMLSTLNINPADIVSKVVTIDEVPEAVKELDQFPERHLKINVKF
                     I"
     aSDomain        complement(1854..2708)
                     /aSDomain="PKS_ER"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_ctg1_50_PKS_ER.1"
                     /evalue="1.20E-12"
                     /label="ctg1_50_PKS_ER.1"
                     /locus_tag="ctg1_50"
                     /protein_end="308"
                     /protein_start="23"
                     /score="39.4"
                     /tool="antismash"
                     /translation="KKDNEVLIKVDAAGICGSDIGAFRGTNPLVTYPRIIGHEVTGIVL
                     ETGEGMPEDIKQGDRVIVDPYIYCGHCYPCSVGRTNCCENLKVIGVHIDGAMQEVVAHP
                     AHLIHKIPDNVPSEMAPLAEPLTIALHALHRAQLKAGEHVVIIGAGAIGLMAALSALRY
                     HATPILVDIVDERLQYAQSLGVPYVVNSAKANVVEAVKDITQGTMAQVVVEASGANSAI
                     RSTLDLASFAGRIAYTGWPKQETSLPTNLITFKELDLRGSRTSAGEFEEALQMLSTLNI
                     NPAD"
     CDS             complement(2749..4038)
                     /gene_functions="transport (smcogs) SMCOG1106:major
                     facilitator transporter (Score: 334.7; E-value: 1e-101)"
                     /gene_kind="transport"
                     /locus_tag="ctg1_51"
                     /transl_table=11
                     /translation="MKRKTMGWLIVFLLFIVYMLNYMDRSALSITAPLIEKELGFNAAE
                     MGMIFSAFFVGYALFNFIGGWASDKVGPKTVFLVAALLWSVFCGMTGLVTGLWTMLAVR
                     VLFGMAEGPVSAAGNKIINNWISRKESATAIGIFSAGSPLGGAISGPVVGLLAISLGWR
                     PAFGIIFIFGLVWVLLWYFFVSDKPSKSKRLAAEERVDFENHDDIILAEDGKGTPSLGY
                     YMKQPMVWATTLAFFSYNYILFFFLTWFPSYLNHSLHLDIKEISIATVIPWVIGAIGMV
                     LGGVCSDFIYRITGNALLSRRLILGICLAGAAVCVAVSGTVTTIGSAVTLMSVSLFLLY
                     LTGPIYWAVIQDVVHKDKVGSVGGAMHGLANISGIIGPLVTGFIVQFSGKYDYAFYLAG
                     AIAIVSSLLVFVFVKNKGLKVNESQSCVHQ"
     CDS             4380..4655
                     /locus_tag="ctg1_52"
                     /transl_table=11
                     /translation="MASVSISCPSCSATDGVVRNGKSTAGHQRYLCSHCRKTWQLQFTY
                     TASQPGTHQKIIDMAMNGVGCRATARIMGVGLNTILRHLKNSGRSR"
     CDS             4719..5066
                     /locus_tag="ctg1_53"
                     /transl_table=11
                     /translation="MSRQRWLFYAYDSLRKTVVAHVFGERTMATLGRLMSLLSPFDVVI
                     WMTDGWPLYESRLKGKLHVISKRYTQRIERHNLNLRQHLARLGRKSLSFSKSVELHDKV
                     IGHYLNIKHYQ"
     CDS             complement(5001..5672)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: Cloacin"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg1_54"
                     /sec_met_domain="Cloacin (E-value: 5.2e-52, bitscore:
                     169.6, seeds: 4, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSDNEGSVPTEGIDYGDTMVVWPSTGRIPGGDVKPGGSSGLAPSM
                     PPGWGDYSPQGIALVQSVLFPGIIRRIILDKELEEGDWSGWSVSVHSPWGNEKVSAART
                     VLENGLRGGLPEPSRPAAVSFARLEPASGNEQKIIRLMVTQQLEQVTDIPASQLPAAGN
                     NVPVKYRLTDLMQNGTQYMAIIGGIPMTVPVVDAGNDSNLLIVFYVQIMPDDFVMQLHR
                     F"
     CDS             5900..6208
                     /locus_tag="ctg1_55"
                     /transl_table=11
                     /translation="MPAGQQTHRLVRLSDSWYVSQLQTMVATLKIPMERRNKRTGRTEK
                     ARIWEVTDRKVRTWIGEAVAAADGVTFSVPVTPHTFRHSYAMHMLYAAEGPAEPDGA"
     CDS             complement(6470..6745)
                     /locus_tag="ctg1_56"
                     /transl_table=11
                     /translation="MVKKPSQQALNRAAVTVEQAEALAQRLADKPYGAPEKPEPEKQCR
                     TTISLGESMLVTIEDLALRNKRNGKDPKNVSAIVRVALEQYLKTLT"
     CDS             complement(6739..7383)
                     /gene_functions="other (smcogs) SMCOG1157:hypothetical
                     protein (Score: 48.4; E-value: 1.5e-14)"
                     /gene_kind="other"
                     /locus_tag="ctg1_57"
                     /transl_table=11
                     /translation="MITVVGGNKGGSGKTTIASNLAIALANKGREVCLLNGDLQRTAAK
                     HHAEREAAGLLPAITLVEKFDNLTQTLQALNEKFDDVIVDVAGRNSKEFITSGVVAHQI
                     IAPLQCSQPDLDTLTELEQQIDAMRNLNPELKVYCLQSMATTNPVLRGNERKEFLEYLE
                     EFPTIQVLDSVICFRKVYRDCMSNGTGVVETNNTAARAEIEHLMNEVFGPW"
     CDS             7612..8583
                     /locus_tag="ctg1_58"
                     /transl_table=11
                     /translation="MNVYCDDGSTTIKLAWNDNGKICKSLSQNSFRHGWKVDGLGIRQT
                     FNYELDGKKYTYDEVSNQSILTTHIEYQYTDVNLLAVHHALLNSGLVPQPVSLTVTLPI
                     SEFYTKECQKNELNIQRKIENLMRPIRLNKGDVFTIEHVDVMPESLPAVFSRLVMDKVG
                     QFEKSLVVDIGGTTLDVGVIVGQFDSVSAIHGNSGIGVSSVTKAAMSALRMASSDTSFL
                     VADELIKRRNDPDFVRQVINDETKTDLVLNTIEGAIASLGEQVVNELGDFHHVNRVYVV
                     GGGAPLIYDSIKTAWHHLGQKVVMMESPQTALVEAIAAFKEE"
     CDS             8588..8980
                     /locus_tag="ctg1_59"
                     /transl_table=11
                     /translation="MDDERKRKKFTLYLHPEKAADFQTLEAIESVPRSERGELFRNAFI
                     SGMALHQLDPRLPVLLTAILSEEFSADQVVTLLSQTTGWKPSQADIRAVLTELGALQSA
                     EKMPPSATDSVQEAMNDVRLKMQKLF"
     CDS             complement(8985..10256)
                     /locus_tag="ctg1_60"
                     /transl_table=11
                     /translation="MFALADINSFYASCEKVFRPDLRNEPVIVLSNNDGCVIARSPEAK
                     ALGIRMGQPWFQVRQMRLEKKIHVFSSNYALYHSMSQRVMAVLESLSPAVEPYSIDEMF
                     IDLRGINHCISPEFFGHQLREQVKSWTGLTMGVGIAPTKTLAKSAQWATKQWPQFSGVV
                     ALTAENRNRILKLLGLQPVGEVWGVGRRLTEKLNALGINTALQLAQANTAFIRKNFSVI
                     LERTVRELNGESCISLEEAPPAKQQIVCSRSFGERITDKDAMHQAVVQYAERAAEKLRG
                     ERQYCRQVTTFVRTSPFAVKEPCYSNASVEKLPLPTQDSRDIIAAACRALNHVWREGYR
                     YMKAGVMLADFTPSGIAQPGLFDEIQPRKNSEKLMKTLDELNQSGKGKVWFAGRGTAPE
                     WQMKREMLSPSYTTQWTEIPIASF"
ORIGIN
        1 atggacgaac agtggggata cgtcggggct aaatcgcgcc agcgctggct gttttacgcg
       61 tatgacaggc tccggaagac ggttgttgcg cacgtattcg gtgaacgcac tatggcgacg
      121 ctggggcgtc ttatgagcct gctgtcaccc tttgacgtgg tgatatggat gacggatggc
      181 tggccgctgt atgaatcccg cctgaaggga aagctgcacg taatcagcaa gcgatatacg
      241 cagcgaattg agcggcataa cctgaatctg aggcagcacc tggcacggct gggacggaag
      301 tcgctgtcgt tctcaaaatc ggtggagctg catgacaaag tcatcgggca ttatctgaac
      361 ataaaacact atcaataagt tggagtcatt accctcttcc atgttcgaag atctggtaaa
      421 agaagaaaac ggtaaagatt ctctgacaca actattaaat cggcgcttta ttccgacgat
      481 tatgcggcgg gaaatctcta tcgccctgca ctcacgtaaa ccgttcgccc tcgtattgat
      541 tacttcaagc aaattaatga tcgttatggg cataataccg gggataatac gctgaaaaat
      601 gtctctgcga tgatttatga acatattcgc agcagtgatt atgttttccg ctatggtggc
      661 gaagaattta tgattttgct tgtcgaaaca gaattatcaa aagccaaaat tattctcgaa
      721 aatctacgga acaaaatggc tgatttgcgt atccaggctt cagccagtga aagtttttcg
      781 gtcacggtca gtattggcgt cgccgaatat gattatcatc ctgactataa gctattgata
      841 gaaaaggcag atagtgcact ttattacgcc aaaagtcagg gccgaaaccg ggtagaagtt
      901 ttccagccac atatgacaaa cggctgattt ttcgtcattg tcactcgaaa gcagtagatc
      961 cgaaccaggg atggtatgat aattcgtcgg ataactaacg ataaaatcga gcgggaggca
     1021 catgctaatt ccagaaatta aacaggaaag gctctatcaa aagatagcga atcttattat
     1081 taagctcatc aatgataatg tgtttccacc aggtagcttt ttacctccag aacgtgaact
     1141 ggcgaaacaa ctgggggtta gtcgttcatc gctaagagaa gcgcttattg ttctggagat
     1201 atctggctgg attattattc agtctggaaa tggggtgatt gtcagcgata aaaaaccccc
     1261 tcatgtcgat tacactatcg aagaaattct ttatacccgt gatttagttg atagtcattg
     1321 tgcccgactt gcagcactgg ctaataatga aacaatcatt aataaaattg aggcacttta
     1381 tttacgaatg gaaaatgcca ttaacgaaaa taatgtggct gaattttata cgcttgataa
     1441 gcagtttcat ctggccattt ctgaagccag tcataatcgg gtgttattag atatgtcacg
     1501 gatgctttgg gagcagcgca tcaatatacc ctatgccggg ctggataaac gttccggcga
     1561 taagaatgta cttatcaacc ttaatcaaca gcataaagca atcattgagg cgattcgtgc
     1621 aggagatgca gacaatgcct tgcaacaggc actgctacat ttgaagtatg tgcgagaaat
     1681 aattggttat taaccttttc tgcgctaaaa aaacgcgtag tgaattcact acgcgttttg
     1741 atttttgcta attaaatgaa tttcacgtta attttcagat ggcgttccgg gaattgatct
     1801 aactctttaa cagcctcagg aacttcatca attgtcacga ctttactcac gatatctgcc
     1861 ggattaatat tcagcgttga taacatttgc agagcttctt caaattcacc ggcactggta
     1921 cgggaaccgc gtaaatccag ttctttaaag gtgatcaaat tagttggtaa cgatgtttcc
     1981 tgttttggcc aacccgtata ggcaatacgc ccggcaaatg aggccagatc cagggtactt
     2041 ctgatcgcgc tatttgcacc agaggcttcc accacaacct gagccatcgt cccttgtgta
     2101 atatctttca ctgcctcaac aacgttagct tttgcggagt taactacgta aggcacaccg
     2161 agcgattgtg cgtattgcaa ccgttcatcg acaatatcaa ccagaatggg ggttgcgtga
     2221 tagcgcaacg cactcagtgc cgccataagt ccaatggcac cagcaccaat aatgacgaca
     2281 tgttcacctg ctttcaattg cgcccgatgt aatgcgtgca gggcaatagt taagggttca
     2341 gccagtggcg ccatttctga cggtacattg tcaggaattt tgtgaattaa gtgagccgga
     2401 tgcgcaacca cttcttgcat agcgccatca atatgtacgc cgatgacttt taagttctca
     2461 cagcaattag ttctgccgac agaacatgga taacagtgac cgcaataaat atagggatct
     2521 acaataaccc ggtcgccctg cttaatatct tctggcatcc cctcaccagt ttccagaaca
     2581 atcccggtga cttcatgtcc aataattcgc ggataagtta ccagtgggtt agtgccacga
     2641 aatgcaccga tgtcagaacc acaaatcccc gctgcatcca ctttaattaa aacttcatta
     2701 tcttttttta ccggataatc gaccaggcga gtggcaattt caccaggttt attgatgtac
     2761 acagctttga ctttcattga ctttcaatcc tttattttta acaaatacga ataccagcag
     2821 acttgaaaca attgctatgg cacctgccag atagaatgcg taatcatatt taccgctgaa
     2881 ctgaacaata aaaccggtta ccaatggacc aataatgccg gaaatatttg ccagcccgtg
     2941 catcgcaccg cctacgctac ctactttatc tttatgcacc acatcctgaa taacagccca
     3001 ataaataggt ccagttaaat aaagcaggaa taaggaaacc gacatcagcg taactgcact
     3061 accaatagtg gtaaccgttc cggaaactgc gacacagact gcggcccccg caaggcaaat
     3121 accgagaatt aagcggcgag aaagtaaggc atttcccgta atgcgataaa taaagtcaga
     3181 gcaaacgcca cctagcacca tgccgattgc accaataacc cacgggataa cagttgcaat
     3241 actaatttct ttaatatcaa ggtgtaacga atggttcaaa tagcttggga accaggtaag
     3301 aaagaaaaac agaatatagt tgtagctaaa gaatgccaga gtagtcgccc ataccatcgg
     3361 ctgtttcatg taataaccca gcgaaggcgt acctttacca tcttcggcca gaataatatc
     3421 gtcatgattt tcaaaatcga cacgctcttc agctgccaga cgtttacttt ttgacggttt
     3481 atcactgaca aagaagtacc acagtaatac ccaaaccagg ccaaatataa agatgatgcc
     3541 aaatgccgga cgccagccaa gtgatatcgc caataagcca acaaccggac cagaaatagc
     3601 accgccaagt ggtgaaccag cactaaaaat accgatggct gttgccgact ctttacgcga
     3661 gatccagtta ttaataattt tattgcctgc tgcactaacc gggccttctg ccataccaaa
     3721 taaaacgcgt acggcaagca tcgtccataa cccggtgacc aggccggtca taccacaaaa
     3781 aacagaccat aacagtgcag caaccaaaaa tactgttttc ggtccaactt tgtcactggc
     3841 ccaaccgcca atgaagttaa ataaagcata accaacgaaa aatgcgctga aaatcatccc
     3901 catttcagca gcattaaagc ctaactcttt ttcaataagt ggggccgtaa tcgataatgc
     3961 agaacgatcc atatagttca gcatgtaaac tataaacaga agaaaaacga taagccatcc
     4021 cattgttttt cttttcatga tatttcctct tgctgtggtc tagcggcctg accacaatat
     4081 ttaatattta tttttagatc atcatgaaaa gaaggatatt gtgatcaata taacgaaaat
     4141 tggataacca gtattcaatg aaaattacaa tcgcgctcta attgcatgat tttgttgatt
     4201 ttatttactc tgttttgagt ggttaatatc acactcagga aataaacaat atggattatt
     4261 ttcatccgga aactaaaaga tgcagtattt aatgcagtaa ggatttattt tacgcataaa
     4321 aataggtgat gctgccaact tactgattta gtgtatgatg gtgtttttga ggtgctccag
     4381 tggcttctgt ttctatcagc tgtccctcct gttcagctac tgacggggtg gtgcgtaacg
     4441 gcaaaagcac cgccggacat cagcgctatc tctgctctca ctgccgtaaa acatggcaac
     4501 tgcagttcac ttacaccgct tctcaacccg gtacgcacca gaaaatcatt gatatggcca
     4561 tgaatggcgt tggatgccgg gcaacagccc gcattatggg cgttggcctc aacacgattt
     4621 tacgtcactt aaaaaactca ggccgcagtc ggtaacctcg cgcatacagc cgggcagtga
     4681 cgtcatcgtc tgcgcggaaa tggacgaaca gtggggctat gtcgcgccag cgctggctgt
     4741 tttacgcgta tgacagtctc cggaagacgg ttgttgcgca cgtattcggt gaacgcacta
     4801 tggcgacgct ggggcgtctt atgagcctgc tgtcaccctt tgacgtggtg atatggatga
     4861 cggatggctg gccgctgtat gaatcccgcc tgaagggaaa gctgcacgta atcagcaagc
     4921 gatatacgca gcgaattgag cggcataacc tgaatctgag gcagcacctg gcacggctgg
     4981 gacggaagtc gctgtcgttc tcaaaatcgg tggagctgca tgacaaagtc atcgggcatt
     5041 atctgaacat aaaacactat caataagttg gagtcattac cggcatccac tactggcact
     5101 gtcatcggaa tacctccgat aatagccata tattgtgtac cattctgcat aaggtccgtc
     5161 agacgatatt ttaccggtac attattaccc gctgctggta actggctggc agggatatcc
     5221 gttacctgct ccagttgctg tgtaaccata agacgaataa ttttttgctc atttccggaa
     5281 gcaggctcca gacgggcaaa agaaacagca gccgggcgag acggttctgg caaaccacca
     5341 cgtaaaccat tttcaagaac tgttcgtgca gcggaaactt tctcgtttcc ccaggggcta
     5401 tgcacactga cagaccatcc cgaccagtct ccctcttcaa gttccttatc aagaataatc
     5461 cggcgaataa ttccaggaaa aagaacactt tgtacaagtg cgataccttg tgggctgtaa
     5521 tccccccatc ccggaggcat ggatggagcg agacctgatg agcctccggg tttcacatca
     5581 ccgcccggaa ttcgtcctgt tgacggccac acaaccattg tgtccccgta atcaatacct
     5641 tctgtcggta cactaccttc attatcactc atagaaaacc tctttgactt taaaaacaat
     5701 caattaaaat taaatgctgt acatataacc actggtttta tgtacagatc aaaaattacg
     5761 ccgctgtgtt ttctctgtca agactgtgga tttcaatttt tgtgaaaacg atcaaaaaac
     5821 aggcactcac accacgcgct attctcgcct gatgccacaa aaaactgcac aagcattact
     5881 ggtcaggacg gcaggaataa tgcccgccgg tcagcagact caccgactgg ttcggctctc
     5941 ggactcctgg tacgtcagcc agctgcagac gatggtggcc acactgaaaa tccccatgga
     6001 acggcgtaat aaacgaacag gcaggacaga gaaagcgcgg atctgggaag tgacggacag
     6061 aaaggtcagg acctggattg gggaagcggt tgctgccgct gatggtgtga cgttctctgt
     6121 cccggtcacg ccacatacgt tccgccattc ctatgcgatg cacatgctgt atgccgctga
     6181 aggtcctgca gagcctgatg gggcataagt ccatcagctc aacggaggtc tacacgaagg
     6241 tgtttgcgct ggatgtggct gcacggcacc gggtgtagtt ttcgatgcct gagtccgatg
     6301 cggtcacaat gctgaaaaac agacacgcat aattcataaa tcataattat gaattgtgat
     6361 ttattctata aaagaagaga ccactgcaat atgtgatctc ttgtatgcaa gggtgcttaa
     6421 acagtgtgaa ttcacaagtg tgatttcata agtaataact tcttgattat tacgttagtg
     6481 tttttaagta ttgctcaagg gctactctga cgatggcact tacatttttt ggatctttac
     6541 cattgcgttt attcctcaat gccagatctt ctattgtgac aagcatgctt tcaccaagag
     6601 agattgtcgt ccgacattgt ttttctggtt cgggtttttc cggcgcgccg tatggtttat
     6661 ccgcaaggcg ctgagccaga gcctctgctt gttctaccgt aactgcagct ctgtttaatg
     6721 cttgctggct gggtttcttc accatgggcc aaatacctca ttcattaaat gttcaatttc
     6781 tgctctggct gctgtattgt tcgtttctac tacaccggtg ccgttgctca tgcaatcgcg
     6841 atagactttt ctgaaacaaa taactgaatc caggacttga attgttggga attcttcaag
     6901 atattcaagg aattcttttc tctcatttcc cctcaaaacc ggattggttg ttgccatact
     6961 ttgtaaacaa taaaccttaa gttccgggtt aaggttacgc attgcgtcta tctgttgttc
     7021 tagttctgta agagtatcca gatctggttg agaacactgt agtggcgcaa ttatttgatg
     7081 tgcgacaact ccactcgtta tgaattcctt tgagtttctt cctgcgacat ccactattac
     7141 atcatcgaac ttttcattca gagcctgtag tgtttgtgtc aggttatcaa atttctcaac
     7201 aagggttatt gctggtaaca gtcctgcagc ttctcgttcc gcatgatgtt ttgcggctgt
     7261 tcgttgtaga tctccgttta acaggcagac ttcccttcct ttatttgcga gagcaatagc
     7321 caggtttgat gcaatggtag ttttccctga accacctttg ttcccaccaa ctacagtaat
     7381 cattgcaacc tcataaatgt gatgtgtgaa gtatgatgat attttgacac ggtaacctga
     7441 tgtgtcaaca tgcattcata tttatgaagt atgaaatatg aagtatgtca tatgatgtaa
     7501 taaatattac ttgtgttttg tgatttcata actatgatag tgctcaaatt gagtattacc
     7561 ttgcaaccag aaacagtgat ttactcagat tgagtattta aggggctata gatgaacgta
     7621 tactgcgatg atggttcaac aacaatcaag ttggcatgga atgacaatgg gaaaatctgt
     7681 aagtcgttgt cgcaaaactc atttcgtcat ggatggaagg ttgacgggtt agggattcgc
     7741 cagacattta actatgaact ggatggtaaa aaatacacgt atgatgaagt tagcaaccaa
     7801 tctattctta cgactcatat tgagtatcaa tatacggatg ttaacctgtt agctgtgcat
     7861 cacgctttac tgaatagtgg attagtgcct cagcctgtat cattgactgt gactttgcct
     7921 atcagtgagt tctacacgaa agagtgtcaa aaaaatgaac tcaacattca gcgtaaaatt
     7981 gagaatttaa tgaggccgat tcgtcttaat aaaggcgatg ttttcaccat tgaacacgtt
     8041 gatgtgatgc ctgaatcatt gccagctgtg ttttctcgtc tggtcatgga taaagttggt
     8101 cagttcgaaa aatcgctggt agttgatatt ggcggcacta cgcttgatgt tggtgttatc
     8161 gttggtcaat ttgactctgt aagtgccatt catggtaatt ctggcattgg tgtgtcttca
     8221 gttacgaaag ctgcaatgag tgcattgcgc atggcatcca gtgataccag tttcctcgtt
     8281 gcagatgaat tgattaaacg tcgtaatgac ccggactttg ttcggcaggt tataaatgac
     8341 gaaacaaaaa ctgatctggt ccttaatacc attgaggggg ctattgccag tctgggggag
     8401 caagtagtca atgagctcgg tgattttcat catgtgaatc gtgtttatgt tgtcggtggt
     8461 ggggccccgt tgatctatga ttcgataaaa acggcatggc accatcttgg gcaaaaagta
     8521 gtgatgatgg agtcgccaca gacagcactg gttgaagcta tcgcggcttt caaagaggag
     8581 taatattgtg gacgatgaac ggaaaaggaa aaaattcaca ctttatctgc atccggaaaa
     8641 agctgcagac tttcaaactt tggaagcgat agagtccgtt ccgcgttctg agcgtggtga
     8701 gctgttccgg aatgcattta tttctggaat ggcgttacac caacttgatc ctcgcctgcc
     8761 tgtattgctg actgccattt tgagcgagga gttttcagca gatcaggttg taaccttact
     8821 cagtcagacg acaggatgga agccttctca ggccgatatc agagctgtgt tgactgaact
     8881 cggtgctttg caatctgctg aaaaaatgcc tccttctgct actgattcgg tgcaggaggc
     8941 aatgaatgat gtgcgcctta aaatgcaaaa attattctga tatatcagaa tgaagcaatg
     9001 gggatctcag tccattgtgt tgtatacgaa ggcgagagca tttcccgttt catttgccat
     9061 tcaggggcgg ttcctcgccc cgcaaaccac actttccctt ttccagactg gttcagttca
     9121 tcgagtgttt tcattaactt ttcgctgttt ttacggggct ggatctcatc aaataatccc
     9181 ggctgcgcta tacccgatgg tgtgaaatca gccagcatga ctcctgcctt catatagcgg
     9241 tacccttcac gccagacatg gtttaacgct ctgcatgctg cggcaataat gtcccggctg
     9301 tcctgtgtgg gtaatggaag cttttccaca gaggcattgc tgtaacaggg ttcttttact
     9361 gcaaaggggg atgtccgtac aaatgtcgtc acctgccggc aatactgacg ctccccacgt
     9421 agtttctctg cggcccgctc tgcatactga acaacagcct ggtgcatggc atctttgtct
     9481 gtgattcgtt caccaaaact gcgactacag acaatctgct gttttgccgg tggtgcttct
     9541 tccagggata tgcaggactc gccgttgagt tcgcgtaccg tacgctcaag aatgacgctg
     9601 aagtttttcc ggatgaatgc cgtgttagcc tgcgccagct gcagtgctgt gttaataccc
     9661 agcgcattca gcttttccgt cagtctgcgt cctactcccc agacctcacc aactggctgc
     9721 agccccagta gcttcaagat ccgattacgg ttttctgccg tcagcgcgac cacaccggaa
     9781 aactgtggcc attgctttgt tgcccactgt gcacttttag ccagcgtttt tgtaggcgca
     9841 atgcccaccc ccatggtgag tcctgtccag ctttttacct gttccctgag ctgatgacca
     9901 aaaaactccg gagagatgca atgatttatc ccccgcaaat caatgaacat ttcatcaatt
     9961 gagtagggct caactgcggg agaaagcgac tccagaacag ccataacccg ttggctcatg
    10021 ctgtggtaca gcgcataatt gctggaaaat acatgtattt tcttctccag gcgcatctgt
    10081 ctcacctgaa accagggctg ccccattctg atgccaaggg cttttgcctc cgggctgcgc
    10141 gcgatcacac agccatcgtt attgctgagt acgatgactg gttcgttgcg aaggtccggg
    10201 cggaaaactt tttcacatga ggcgtagaaa ctgttgatat cagccagtgc aaacatcagc
    10261 gtaactcccg cgtcctgtgt atcacgtgag tgacaacacc aaaaatacag atgttttccg
    10321 gatacagtgt gcggaactcc gggctgtctg aaaccggctc cagtgccggg cgtgggcgca
    10381 acagcagtcg tttgacggtg aactcaccgt cgatctcagc gataacgatg tccccgtgtt
    10441 gtggtttttc ggccctgtcc actaccagca gatcaccatt ctgcacgcca gcctggttca
    10501 tcgattcacc gctggcgcgc agaaagaagg tggctgcagg tctgctgatg caatagctgt
    10561 tcagatccag ttcctgctca gcataatcag tggcaggcga aggaaaaccg gcctggcaac
    10621 gatcggcaaa caacggtcgc acgtaactgt catcgcctga agggtcagcc gg
//
