NZ_AP014865.1 - 2 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

NZ_AP014865.1

Region	Type	From	To	Most similar known cluster		Similarity
Region 1	lanthipeptide-class-ii,RiPP-like	85,270	109,056
Region 2	NRPS,T1PKS	143,998	248,533

NZ_AP014865.1 - Region 1 - RiPP-like,lanthipeptide-class-ii

Download region GenBank file

Download region SVG

Location: 85,270 - 109,056 nt. (total: 23,787 nt) Show pHMM detection rules used

lanthipeptide-class-ii: cds(LANC_like and DUF4135)
RiPP-like: (strepbact or Antimicrobial14 or Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or Lactococcin or Antimicrobial17 or Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Lanthipeptides

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Lanthipeptide predictions

Lanthipeptide(s) for ctg1_95

allorf_089417_089569

Leader:

MQITAQIRLRKRTKGLMIKALQGM

Core:

QGRTKGIEELSILKVKDFLECFNFNT

Core with dehydrations:

QGRDhbKGIEELDhaILKVKDFLECysFNFNDhb

antiSMASH-DB 4.0 matches for allorf_089417_089569

20.7% NZ_CP021707.1: Lanthipeptides: allorf_3956612_3956845_lanthipeptide

2

GRTKGIEELS

11

GRT+ I E+S

13

GRTQPIPEMS

22

allorf_090602_090685

Leader:

MIKLIAIIGG

Core:

TNVIWEASCLLSILQKS

Core with dehydrations:

DhbNVIWEADhaCysLLDhaILQKDha

antiSMASH-DB 4.0 matches for allorf_090602_090685

35.0% NZ_QGGL01000004.1: Lanthipeptides: allorf_252800_252931_lanthipeptide

5

WEASCLLSIL

14

W ASC SIL

2

WSASCFCSIL

11

allorf_105487_105594

Leader:

MLNYIYPHKQKRVDLLGS

Core:

TLTFLFFYFSLMFICRN

Core with dehydrations:

DhbLDhbFLFFYFDhaLMFICysRN

Legend:

Dha: Didehydroalanine
Dhb: Didehydrobutyrine

Lanthipeptides

Core peptide predictions

allorf_089417_089569 - Class II

Cleavage pHMM score: -6.20

RODEO score: 0

Monoisotopic mass: 2974.6 Da

Molecular weight: 2976.4 Da

Number of bridges: 1

Alternative weights: (assuming N unmodified Ser/Thr residues); 2994.4 Da N = 1; 3012.4 Da N = 2

allorf_090602_090685 - Class II

Cleavage pHMM score: -2.90

RODEO score: 0

Monoisotopic mass: 1832.0 Da

Molecular weight: 1833.1 Da

Number of bridges: 1

Alternative weights: (assuming N unmodified Ser/Thr residues); 1851.2 Da N = 1; 1869.2 Da N = 2; 1887.2 Da N = 3

allorf_105487_105594 - Class II

Cleavage pHMM score: -3.20

RODEO score: 0

Monoisotopic mass: 2108.1 Da

Molecular weight: 2109.5 Da

Number of bridges: 1

Alternative weights: (assuming N unmodified Ser/Thr residues); 2127.6 Da N = 1; 2145.6 Da N = 2

NZ_AP014865.1 - Region 2 - NRPS,T1PKS

Download region GenBank file

Download region SVG

Location: 143,998 - 248,533 nt. (total: 104,536 nt) Show pHMM detection rules used

NRPS: cds(Condensation and (AMP-binding or A-OX))
T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

NRPS/PKS domains

NRPS/PKS modules

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Detailed domain annotation

Selected features only

Show module domains

Module view

Legend

NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 4, location 143997 - 248533:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X - Tyr) + (ohmal) + (bOH-Tyr - Pro) + (X - ohmal) + (D-Asn) + (X - X)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

ctg1_156

AMP-binding (280..682): X

nrpys: Val, Leu, Ile, Abu, Ival

SVM prediction details:

Predicted physicochemical class:: N/A
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DVFYFGLVCK
Nearest Stachelhaus code(s):: Met DVFYLGGVFK (59% 8Å match); Ala DVFFVGLVAK (50% 8Å match); Pro DLFYLALVCK (47% 8Å match)
Stachelhaus code match:: 70% (weak)

AMP-binding (1323..1725): X

nrpys: (unknown)

SVM prediction details:

Predicted physicochemical class:: N/A
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DVFYLGGVCK
Nearest Stachelhaus code(s):: Met DVFYLGGVFK (62% 8Å match)
Stachelhaus code match:: 90% (moderate)

ctg1_158

Search NORINE for peptide: strict or relaxed

AMP-binding (461..856): Asn

nrpys: Asn

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: asp,asn (Asp, Asn)
Single AA prediction:: asn (Asn)

Stachelhaus prediction details:

Stachelhaus sequence:: DLTKIGEVGK
Nearest Stachelhaus code(s):: Asn DLTKIGEVGK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

ctg1_162

AMP-binding (448..845): X

nrpys: (unknown)

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Aliphatic chain or phenyl group with -OH (Ser, Thr, Dhpg, Hpg)
Small clusters prediction:: thr (Thr)
Single AA prediction:: thr (Thr)

Stachelhaus prediction details:

Stachelhaus sequence:: DMWNTGMVHK
Nearest Stachelhaus code(s):: Ser DMWNTGLVHK (82% 8Å match)
Stachelhaus code match:: 90% (moderate)

PKS_AT (1551..1847): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 83.3%; 2-Rhyd-Malonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 133.6
inactive: 95.1
Methoxymalonyl-CoA: 43.2
Methylmalonyl-CoA: 24.0
Propionyl-CoA: 15.4
Isobutyryl-CoA: 9.4
trans-1,2-CPDA: 0.0
fatty_acid: 0.0
Ethylmalonyl-CoA: 0.0
CHC-CoA: 0.0
Benzoyl-CoA: 0.0
Acetyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0
2-Methylbutyryl-CoA: 0.0

ctg1_163

Search NORINE for peptide: strict or relaxed

AMP-binding (8..410): Ser

nrpys: Ser

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Aliphatic chain or phenyl group with -OH (Ser, Thr, Dhpg, Hpg)
Small clusters prediction:: ser (Ser)
Single AA prediction:: ser (Ser)

Stachelhaus prediction details:

Stachelhaus sequence:: DVWHFSLIDK
Nearest Stachelhaus code(s):: Ser DVWHFSLIDK (91% 8Å match)
Stachelhaus code match:: 100% (strong)

ctg1_167

PKS_AT (5..291): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 62.5%; 2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 54.8
Methoxymalonyl-CoA: 32.2
inactive: 24.9
Methylmalonyl-CoA: 14.8
trans-1,2-CPDA: 0.0
fatty_acid: 0.0
Propionyl-CoA: 0.0
Isobutyryl-CoA: 0.0
Ethylmalonyl-CoA: 0.0
CHC-CoA: 0.0
Benzoyl-CoA: 0.0
Acetyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0
2-Methylbutyryl-CoA: 0.0

ctg1_172

Search NORINE for peptide: strict or relaxed

AMP-binding (274..685): bOH-Tyr

nrpys: bOH-Tyr

SVM prediction details:

Predicted physicochemical class:: N/A
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DAQDIAAVDK
Nearest Stachelhaus code(s):: bOH-Tyr DASTIAAVDK (62% 8Å match)
Stachelhaus code match:: 80% (moderate)

AMP-binding (1329..1728): Pro

nrpys: Pro

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Cyclic aliphatic chain (polar NH2 group) (Pro, Hpr)
Small clusters prediction:: Tiny, hydrophilic, transition to aliphatic (Gly, Ala)
Single AA prediction:: pro (Pro)

Stachelhaus prediction details:

Stachelhaus sequence:: DVLNLAMVIK
Nearest Stachelhaus code(s):: Ala DVHNLAIVYK (76% 8Å match); Ser DVWNVAMVHK (62% 8Å match)
Stachelhaus code match:: 70% (weak)

ctg1_173

PKS_AT (1897..2192): mal

ATSignature: 2-Rhyd-Malonyl-CoA

Top 3 matches:: 2-Rhyd-Malonyl-CoA: 95.8%; Malonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 107.2
inactive: 74.4
Methoxymalonyl-CoA: 52.2
Methylmalonyl-CoA: 43.4
Propionyl-CoA: 32.6
CHC-CoA: 15.8
Ethylmalonyl-CoA: 15.0
2-Methylbutyryl-CoA: 13.5
Isobutyryl-CoA: 12.6
3-Methylbutyryl-CoA: 10.0
trans-1,2-CPDA: 0.0
fatty_acid: 0.0
Benzoyl-CoA: 0.0
Acetyl-CoA: 0.0

ctg1_177

Search NORINE for peptide: strict or relaxed

AMP-binding (477..875): X

nrpys: (unknown)

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aromatic (Phe, Tyr, diOH-Bz, Ph-Gly, bOH-Tyr)
Large clusters prediction:: Aromatic side chain (Phe, Trp, Ph-Gly, Tyr, bOH-Tyr)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DAMVFGAVCK
Nearest Stachelhaus code(s):: Leu DAMLVGAVCK (71% 8Å match)
Stachelhaus code match:: 80% (moderate)

AMP-binding (1538..1942): Tyr

nrpys: Tyr

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Aromatic side chain (Phe, Trp, Ph-Gly, Tyr, bOH-Tyr)
Small clusters prediction:: Polar aromatic ring (Tyr, bOH-Tyr)
Single AA prediction:: tyr (Tyr)

Stachelhaus prediction details:

Stachelhaus sequence:: DSSDIGEVSK
Nearest Stachelhaus code(s):: Lys DAEDIGTVSK (65% 8Å match)
Stachelhaus code match:: 70% (weak)