LOCUS       CP128701.1              7915 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  CP128701.1 Klebsiella pneumoniae strain KPN23 plasmid pKPN23_2,
            complete sequence.
ACCESSION   CP128701
VERSION     CP128701.1
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##antiSMASH-Data-START##
            Version      :: 7.1.0
            Run date     :: 2024-06-07 18:02:57
            NOTE: This is a single region extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 7915
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..7915
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="True"
                     /core_location="[1229:2915]"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      1230..2915
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     cand_cluster    1..7915
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     region          1..7915
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="RiPP-like"
                     /region_number="1"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     CDS             complement(2..88)
                     /locus_tag="ctg1_1"
                     /transl_table=11
                     /translation="MEKRHRRGPAVLPHMLVPCLSTESVGNFV"
     CDS             complement(729..878)
                     /locus_tag="ctg1_2"
                     /transl_table=11
                     /translation="MKKVKTIFLFILIVAGFLLAACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
     CDS             complement(963..1220)
                     /locus_tag="ctg1_3"
                     /transl_table=11
                     /translation="MGLKLRLTWFDKKTEEFKGEEYSKDFGDDGSVIESLGMPLKDNIN
                     NGEFDLKKEWVPLLQPFFKNQIKPDVDNCYIAFDYRDGNW"
     CDS             complement(1230..2915)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: Cloacin"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg1_4"
                     /sec_met_domain="Cloacin (E-value: 1.6e-110, bitscore:
                     357.5, seeds: 4, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IMTSLPADAVTETPASTLPLDQATVRVRQRVVDVVKDERQHIAVVAGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPTAKAPPKGIVAEKGDSRPAGFTAGGNSREAVIR
                     FPKETGQKPVYVSVTDVLTPAQVKQRQEEEKRRQQAWDAAHPEEGLKRDYDKAKAELDA
                     EDKNIATLNSRIASTEKALPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQASLNAMNESLSRDKAALTGAMESRKQKEKKAKDAENKLNE
                     EKNKPRKGTKDYGHDYHPVPKTEDIKGLGELKRGDPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
     CDS             3242..3487
                     /locus_tag="ctg1_5"
                     /transl_table=11
                     /translation="MTHLKLTPAEEKRLADAFVAQALAMGLKGNKTLPKAVKQQVRERV
                     LRAIQDEREARRAQEMRREKYEATETTFTWQPQRRR"
     CDS             3761..4132
                     /locus_tag="ctg1_6"
                     /transl_table=11
                     /translation="MRLIPFILLALCSPLLLTACKHSTTQPPKIYTLDKSSKISSYAAA
                     IKTAVEMQMFEPEAYAGKMCTVRISIQRDGTLNSAIAEGGDPKLCKAAISAVTHAKIPP
                     APDENTWQIFKNAPLDFRP"
     CDS             4129..4494
                     /locus_tag="ctg1_7"
                     /transl_table=11
                     /translation="MKAFTLTVLAAMFLTPVHAIPLKPVTTLQPTYPVQAAAKRIEGQV
                     TATFDILDDGRVDNVSLTGCHLFFRETLTAMRHWLYPAGHPAMRQVINFEFSLKNDGLQ
                     DPRPVTTQKLNDDGCRQ"
     CDS             4669..4947
                     /locus_tag="ctg1_8"
                     /transl_table=11
                     /translation="MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEK
                     RSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWGTENEQDAPPCHR"
     CDS             4935..5249
                     /locus_tag="ctg1_9"
                     /transl_table=11
                     /translation="MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKT
                     LPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDREMDQIVIVTVRHQKESGYPGADSL
                     "
     CDS             5413..5841
                     /locus_tag="ctg1_10"
                     /transl_table=11
                     /translation="MAKTLLNLSQAAQAAGITRRTLYNHVKQGKVTVSRDGKNNPVVDV
                     SELIRVYGNVNIPEKQIPGISHRENTQKNFPQEQLLAMQKELADLRQAVTLMLEDKTSR
                     EEERRQHDDERRKLQAEVDRLTTELTQKKKRFWSGWFS"
     CDS             5867..6046
                     /locus_tag="ctg1_11"
                     /transl_table=11
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
     CDS             complement(6073..6603)
                     /locus_tag="ctg1_12"
                     /transl_table=11
                     /translation="MALYMVFIEDVEDKDGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGENPQWERDKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIEVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
     CDS             complement(6610..7341)
                     /locus_tag="ctg1_13"
                     /transl_table=11
                     /translation="MTLEQYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDVIPFQP
                     SRVSAAQLPHRLAVQSLRLSMEGRGATGWRYLHRMALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRRANGIDEIWYICPDRPTLVRVQRAILAVDEIVNPQTGEA
                     RKTVELDRERLFACFKFMTTE"
ORIGIN
        1 atacaaagtt acccacagat tccgtggata agcaggggac taacatgtga ggcaaaacgg
       61 cagggccgcg ccggtggcgt ttttccatag gctccgccct cctgccagag ttcacataaa
      121 cagacgcttt ttcggtgcat ctgtgggagc cgtgaggctc aaccatgaat ctgacagtac
      181 gggcgaaacc cgacaggact taaagatccc caccgtttcc ggtaggtcgc tccctcttgc
      241 gctctcctgt tccgaccctg ccgtttaccg gatacctgtc cgccgttctc ccttgcggga
      301 agtgtggcgc tttctcatag ctcacacact ggtatctcgg ctcggtgtag gtcgttcgct
      361 ccaagctggg ctgtaagcaa gaactccccg ttcagcccga ctgctgcgcc ttatccggta
      421 actgttcact tgagtccaac ccgggaaagc acgcaaaacg ccactggcag cagccattgg
      481 taactgggag ttcgcagagg atttgtttag ctaaacacgc ggttgctctt gaagtgtgcg
      541 ccaaagtccg gctacactgg aaggacagat ttggttgctg tgctctgcga aagccagtta
      601 ccacggttaa gcagttcccc aactgactta accttcgatc aaaccacctc cccaggtggt
      661 tttttcgttt acagggcaaa agattacgcg cagaaaaaaa ggatctcaag aagatccttt
      721 gatcttttct actgaaccgc gatccccgtc agttcagagg aggacgatgg tgccaccgtt
      781 ccgccctgaa catcccggat ataatttgcc tgacatgcgg ccagaagaaa gccagcaaca
      841 atcaatataa aaagaaaaat agtctttacc tttttcatat actttccctg tatttaaaga
      901 ctccggcatt catccctgaa caccggaagt cagtatttca aatcatcctt gataattttt
      961 tgttaccagt taccgtcccg ataatcaaaa gctatataac aattatccac atcaggtttt
     1021 atttgatttt taaaaaatgg ctgtagtaaa ggaacccatt ctttcttcaa atcaaactca
     1081 ccattattaa tattatcctt taaaggcatt cccaagcttt caatgacaga accatcgtca
     1141 ccaaagtctt tagaatactc ttccccttta aactcttcag ttttcttatc aaaccatgtc
     1201 aaacgtaatt taagccccat atttacctct caaagatatt ttttaatgtt ccgttttgga
     1261 tctggccctt taacctgctt acccgttttg ggatcaaatg cgcctaggtg ttcgccatca
     1321 ctggcacgat acccttcaag ctcaccgtgc tgggaatccc attcataaat tttacgtttt
     1381 ttatcgccat accatcgggc ccgcttacca ccaccacctt gtttaggtgt tttaggatca
     1441 cctcgcttta actctccaag tccctttatg tcttctgttt taggtactgg atgataatca
     1501 tggccgtagt ctttagtccc cttgcgaggc ttgttctttt cctcatttaa cttattttct
     1561 gcatccttcg cttttttctc cttttgtttc cggctctcca tcgccccggt aagtgcagcc
     1621 ttatctctgg ataaggattc attcattgcg tttaaagatg cctgagccgc ttttaatttc
     1681 tcattctgat tctgaatatc cttatcaaga tagcgaacct gatcctgcca cccggagcca
     1741 tattcatgag gcgggttata agtcacaaaa tcatctttat ttgcctctgc ctcttttacc
     1801 tttttatcgg cttcctgaac agcagccctt gcaccgggga gcgccttctc tgtcgatgca
     1861 atgcggctgt ttaaggtcgc aatattttta tcttcggcat ccagctcagc tttcgcttta
     1921 tcatagtctc ttttcagccc ctcttccgga tgagcggcgt cccatgcctg ctggcgacgc
     1981 ttttcttcct cctgacgctg ttttacctgt gccggggtaa gaacatctgt caccgacaca
     2041 taaaccggct tctgtccggt ctctttcggg aaacgaataa cggcctcacg ggagttacca
     2101 ccggctgtaa aaccagccgg acgtgaatca cctttttcag caacaatgcc ttttggcggg
     2161 gctttcgctg tcggaacacc tttcggtacg ctcacctgca gagacgggag acccggaatc
     2221 gacacactga atacccccgg acgttttgtc ggtttcgcat ccaccacagg gacactcatt
     2281 ggccgacctg cgacaaccgc aatatgctgc cgctcatcct tcaccacatc cacaacccgt
     2341 tgtctgacac gaaccgtcgc ctggtccagt ggtaaagtac ttgccggagt ctccgtcacc
     2401 gcatcggccg gtaatgacgt cataattttt gacatcattt tcggatcatc ttttgcaatc
     2461 tcagaaggca gcacaccgta gatcgcaatc ccccacagac caaacttaaa cggccccttc
     2521 agggcagcca gcacatcagc aacggcggcc gacaacgcat cgccggaaac tgataaagcc
     2581 agtccttcag cccccggagt agccagggca ggcagaccga aggccatcgc agtggccgaa
     2641 gactgtccgc caccagagct accgctatga ttgccgctgt taccaccatt accaccgggt
     2701 ttactcccgc cgttaccaaa ctcccccgga ttatagttat gaatatctcc attcggcgta
     2761 tgcgtcgtac cccagcccgg cccgctgttt ggatccgtac caccacctga tgaagggcca
     2821 cctttaccag acgtaccgtt cacattccca ctggcctgac caccattatg tcccagacct
     2881 gaattacccg gacctcgacc gtctccacca ctcataccgt tttcctcttt gacatttaaa
     2941 acaataaatt aaagccaaca tactgtacat atacacagta taattattac cttactcctc
     3001 ccgttttcag gtgtcaatat atgagattag ttctcatctt caggcacaaa aaagcgcccg
     3061 gagccacctg taagcagatc gcgtcttttc ccgaaaattt ctcatggcaa caaattttat
     3121 tatttaagac attcatcaca cttacatttc agatggtata gacacatctg aaagctaact
     3181 ggcatgagga gaggacctgc ggttaaactg ttcgggaaac ctgagacgcc cggaggccac
     3241 tatgacgcac ctgaaactga ctcccgctga agaaaagcgc ctggcggacg cctttgtcgc
     3301 tcaggcactg gccatgggcc tgaaagggaa caaaacgctg ccaaaggcgg taaaacaaca
     3361 ggtgcgggaa agggtgcttc gtgccattca ggacgaacgt gaagccaggc gggcgcagga
     3421 aatgcgtcgg gagaaatacg aggctacgga aaccaccttc acctggcagc cacagcgccg
     3481 ccggtaaggg gtatttacca taacgtcgca ttgcccgcac cgctcagacg gtctgagggc
     3541 gtttccggcg gctacgggat aaaacaggca acaaaccacc cggaaaaccg ccggaaacgc
     3601 gcccggtaaa ttgtaacccg ctgcatgcct atgcacgcaa ctgcatggcg ggccgtttgg
     3661 tgtaaaaacg atacgataag ccgtgtttat gtaaatgaac gtggccgtaa agagtgacaa
     3721 atatacatgt aacatttttt actgacaggt catcaacccg atgcgtttaa taccctttat
     3781 cctgctggca ttgtgcagcc ccttactcct gacggcctgt aagcattcca ccacccagcc
     3841 gccgaaaatt tatactctgg ataagtcatc taaaatcagt agctacgctg cggccatcaa
     3901 gaccgctgtt gaaatgcaga tgtttgaacc agaggcctat gcgggaaaaa tgtgcaccgt
     3961 gcggatatcc atacaacgtg atggcactct gaacagcgcc attgctgaag gaggagatcc
     4021 gaaactctgt aaagcagcaa tatcagccgt tacccacgcc aaaattccgc cggcaccgga
     4081 tgaaaatacc tggcaaatat tcaaaaacgc ccctctggat ttcagaccat gaaagctttt
     4141 actctgacag tgctggcggc catgttcctc actccggtac atgcgatacc actaaaaccc
     4201 gtaaccacac tgcagccaac ctacccggta caggccgcag caaaacgcat cgaaggtcag
     4261 gtgaccgcca cgtttgatat tctggacgat ggacgagtgg acaacgtgtc gcttaccggc
     4321 tgtcaccttt tttttcggga aacgctgacg gcaatgaggc actggctata tcccgcaggg
     4381 cacccggcaa tgcgacaggt tataaatttt gaattcagtc tgaaaaatga cggcttacag
     4441 gatccgcgac cagtcaccac ccaaaagctc aatgatgatg gttgccgcca gtaacaggtc
     4501 ctttaacccg caggctgcaa tagtcctgga tgatgagtga gcctgtagca gccctgatta
     4561 gtggaagcga agctgaaaaa tgggcagcaa aagccgtatt tctggcaaat agcgccgcca
     4621 cggattaaag tagtcatgtg taatacatat gactacaaag gggcttttat gtcatcagca
     4681 acatccatca ggctcgatga agaacttaag gaccggctga aaacgctggc cgatgaccgg
     4741 caccgctcag cgcacgcgct aatgctggag gcaatcaccg aatacatcga ccgggaagag
     4801 aagcgcagcc agtacctgcg ggacggacag gccgcatggc agcactatca ggaaacgggt
     4861 ctgcacctta ctgcggaaga agcggaagcc tggataagta cctggggaac tgagaacgag
     4921 caggatgctc cgccatgcca caggtaacga tttccgcgct ggcacagcgc gatttacaac
     4981 gccttcagga ctttctcaaa accaaaaatc ggctggcagc cagaaaagga ggtgaggtga
     5041 tcgtccgggc tatccagcaa ctgaaaacat tgccagacat tggccgcccg gtgccgtttc
     5101 tgccgctgga atataaggaa ctggtgatcg ggtttggcga cagtggctat gtgatgctct
     5161 accgccacga ccgggaaatg gaccagattg tgatcgtcac cgtcaggcat cagaaagaat
     5221 ccgggtatcc gggggcagac agcctctaat aaccgatgac gggggcgcaa ttcattgcgt
     5281 ggccgtcgcc ggtttgacaa gcgcagcgcg tcagaaaacc acatcctgaa cggaaaaacg
     5341 ggtaaaattc agcgatacat ttaccgtgag aaattacaca cctttcacac ccgataaaat
     5401 caggggtata cagtggcgaa aacattgctc aacctttcac aggcagcaca agccgcgggc
     5461 attaccagaa gaacgctata caatcatgta aaacagggaa aggttaccgt gtcacgtgac
     5521 gggaaaaaca accctgtcgt ggatgtgtca gaactgatcc gtgtatacgg aaatgtgaat
     5581 atccctgaga aacaaattcc cggcatttca caccgtgaaa atacacaaaa aaatttccca
     5641 caggaacagc ttctggcaat gcaaaaggaa ctggccgatc tccggcaggc tgtcaccctg
     5701 atgctggaag ataaaacatc ccgggaagaa gaacgccgcc agcacgatga tgagcgccgg
     5761 aaactccagg ccgaagtgga cagattgaca acagaactga cacagaagaa aaaacgcttc
     5821 tggtccggct ggtttagctg agaacttacc gaagggaatg gagaacatga cgaagcagga
     5881 aaaaactgcc ctcaatatgg ctaaattcat taaggatcag tcgctgctgt tgctggaaaa
     5941 gctgaacgag ctggacatgg ataccccggc cgacatgtgc gaacggcttc acgaggatgc
     6001 ggaaacactc tggcaggtga tgcgggataa gctcggggaa gaataacagc accggccatg
     6061 ccggtgctgg cgttattttt tgccgaacgg ccaccaccgg cgggcgggtt tgtcctccgg
     6121 cagttcaggc accggcacgt taaaatagcg gctgttatat tcccgcatca gctccgcttt
     6181 cagcttctcg ttcaccgcgc tttccccgtg ccgctcgctg accacctcaa tcatcgcgtt
     6241 aatctgctcc ggtgagcact tcccggcatc aaccacctgc acaccgttct cgatggcgaa
     6301 ccgctccacc tttcgataga ggtaatcaca cagcttcatt cctgtgggtt tgtcccgttc
     6361 ccattgcgga ttttcaccgg tgaaaaagta cgtggctttt tcgtcgctgt tgagccgctc
     6421 gtcatacatc ccgatggatt tcatcatcgc cagatgctgg gcccggtcga caaaacagaa
     6481 aacatacgcc ccggtctgcc aggcgagcac caccatgtct tccagatgct cgctggttgg
     6541 cataatgcag cgctcgccgt ctttgtcttc cacgtcctca ataaatacca tgtacagcgc
     6601 cataatccct cactccgttg tcataaattt aaagcacgcg aacagcctct cccggtccag
     6661 ctccaccgtt ttgcgggcct cgccggtctg tggattcacg atttcatcca ccgccagaat
     6721 cgcccgctgt acccggacaa gcgtcggccg gtcagggcag atgtaccaga tttcatcgat
     6781 gccgttcgcc cggcggttga acagataccc ggacaccact tcctgatacc gcctgcggct
     6841 cttgacggtg cgctctacct caaatgccac cgttctgccg tccagctccg ccagcgcgtc
     6901 cggcactttc atccctttca gggccatacg gtgcaggtac cgccagccgg tggccccccg
     6961 gccctccatg gagaggcgca gggactgcac ggccagccgg tgcggcagct gcgccgccga
     7021 cacccttgac ggctgaaacg gaataacgtc cgtcagcggc tcgtcctcgt cgaacgagaa
     7081 cgccaccccg tgcggcgtca gcccccacac gctgacctga cggccgtcaa ccgggtattt
     7141 cgcccggacc agcagccctt tcgccaccag actgtccagg aagcggtaaa ccggctgtac
     7201 cgccgcaatc cccagccgtc gcgcaaccag ctccgccgtg gaacacagct cttcccgcag
     7261 ccagcgcagg gtcagccgtg ttttctccgc gttccgcgcc tgccgcttcg cgtggctgac
     7321 gttgtactgc tccagtgtca tcagtaaagc cccgcaaaat catcatccgc ttttggtccg
     7381 ccgccgtcgg ccggaggcag gtactccgcc agcgggtccg ttttcgccag caccgggaag
     7441 gttttcagcg aaatttcccg gcgctcaacc cgcagcgggc tgctgaacgc cagccgcgcc
     7501 acgcccagcc cggtcatcac cgcgaacccg tcaggcaggt gttgcagggt gttcacgtca
     7561 aacagcggcc gcgcctcctt ctggatgaag ctgtcgccgc tgatgtgctc cacgttcccc
     7621 gcctcccggc tggcccggcg gcgctccacg tccacccgga tctccccggt ctggcccgcc
     7681 gcccactgcg cggactcctg gctggccgcg cgatagaacc agcggatcgg cgtgttatcc
     7741 agtaccgtgg ttttcacaaa atcagcgggc agatcctgcc cgcactgacc gaagtcgccc
     7801 agcgactggt gcgcgagcag caggttgcag tcccggctgc gcagcgtccc cagcgcgttc
     7861 agcacgtact tcgacagcag atatttaatc tcatcgagca tgatgctcgc gtgcg
//
