CP098529.1 - 2 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

CP098529.1

Region	Type	From	To	Most similar known cluster		Similarity
Region 1	T1PKS	1	23,597
Region 2	NRPS,T1PKS	42,919	98,093

CP098529.1 - Region 1 - T1PKS

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Location: 1 - 23,597 nt. (total: 23,597 nt) Show pHMM detection rules used

Region on contig edge.

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

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Gene details

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Gene overview

NRPS/PKS domains

NRPS/PKS modules

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Detailed domain annotation

Selected features only

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NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 0 - 23597:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (mal)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

ctg1_2

PKS_AT (533..807): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 70.8%; inactive: 58.3%; Methylmalonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 74.8
Methylmalonyl-CoA: 63.2
Methoxymalonyl-CoA: 53.7
inactive: 41.1
CHC-CoA: 34.4
2-Methylbutyryl-CoA: 32.7
Ethylmalonyl-CoA: 29.2
Propionyl-CoA: 28.4
trans-1,2-CPDA: 25.5
Isobutyryl-CoA: 16.6
Acetyl-CoA: 16.4
Benzoyl-CoA: 16.1
fatty_acid: 13.7
3-Methylbutyryl-CoA: 10.2

CP098529.1 - Region 2 - NRPS,T1PKS

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Location: 42,919 - 98,093 nt. (total: 55,175 nt) Show pHMM detection rules used

Region on contig edge.

NRPS: cds(Condensation and (AMP-binding or A-OX))
T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

NRPS/PKS domains

NRPS/PKS modules

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Detailed domain annotation

Selected features only

Show module domains

Module view

Legend

NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 2, location 42918 - 98093:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X - X - X) + (X - X - X) + (mal)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

ctg1_84

AMP-binding (472..853): X

nrpys: Gly, Ala

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Tiny, hydrophilic, transition to aliphatic (Gly, Ala)
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DVLCMVLIIK
Nearest Stachelhaus code(s):: Leu DVLIFGLIYK (56% 8Å match); Ala DVWCTAAIIK (53% 8Å match); Ser DVWHMSLIDK (50% 8Å match)
Stachelhaus code match:: 60% (weak)

ctg1_88

PKS_AT (519..812): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 66.7%; inactive: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 83.5
inactive: 46.6
Methylmalonyl-CoA: 32.4
Methoxymalonyl-CoA: 29.9
CHC-CoA: 16.1
Propionyl-CoA: 15.1
Acetyl-CoA: 14.2
2-Methylbutyryl-CoA: 11.2
Isobutyryl-CoA: 10.7
fatty_acid: 8.3
trans-1,2-CPDA: 7.9
Benzoyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0
Ethylmalonyl-CoA: -1.5

ctg1_90

AMP-binding (495..898): X

nrpys: Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DMYVLGVMWK
Nearest Stachelhaus code(s):: bAla DMLQLGVVWK (44% 8Å match)
Stachelhaus code match:: 70% (weak)

AMP-binding (1551..1965): X

nrpys: Phe, Tyr, diOH-Bz, Ph-Gly, bOH-Tyr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aromatic (Phe, Tyr, diOH-Bz, Ph-Gly, bOH-Tyr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: VDGTFSVADK
Nearest Stachelhaus code(s):: bAla VDGVVSFADK (74% 8Å match)
Stachelhaus code match:: 70% (weak)

AMP-binding (2613..3041): X

nrpys: Val, Leu, Ile, Abu, Ival

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DHPWLASTCK
Nearest Stachelhaus code(s):
Stachelhaus code match:: 0% (weak)

ctg1_91

AMP-binding (314..717): X

nrpys: Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DMYVLGVMWK
Nearest Stachelhaus code(s):: bAla DMLQLGVVWK (44% 8Å match)
Stachelhaus code match:: 70% (weak)

AMP-binding (1932..2346): X

nrpys: Phe, Tyr, diOH-Bz, Ph-Gly, bOH-Tyr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aromatic (Phe, Tyr, diOH-Bz, Ph-Gly, bOH-Tyr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: VDGTFSVADK
Nearest Stachelhaus code(s):: bAla VDGVVSFADK (74% 8Å match)
Stachelhaus code match:: 70% (weak)

AMP-binding (3001..3425): X

nrpys: Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: PLYWMGSTCK
Nearest Stachelhaus code(s):: Kiv GLYWLGSSGK (47% 8Å match)
Stachelhaus code match:: 60% (weak)