The BusyBee webserver was developed to enable the convenient analysis of metagenomic data in the form of assembled contigs or long reads (PacBio or ONT). To this end, the webserver currently provides unsupervised (i.e., reference-independent) binning, binning quality assessment, functional annotation of antibiotic resistance genes, and taxonomic annotation. The only required input are sequences in FASTA-format and all analyses are performed automatically. Upon completion, the results are visualized, thereby enabling intuitive user inspection. Moreover, a dump of the results can be downloaded.

How to use it?

We provide a detailed tutorial, F.A.Q.s, and per-site step-by-step help (see "Help" in the top navigation bar). Should you have further questions, please do not hesitate to contact us.


The results generated by the BusyBee webserver are available for 14 days upon job completion. Should you require your results to persist longer, please contact us. We might change this policy in the future depending on the availability of computational resources and server demand. Moreover, certain file size constraints are installed to assure the general availability of this webservice. If you require larger datasets to be run, please contact us.


Schmartz, P. G., Hirsch, P., Amand, J., Dastbaz, J., Fehlmann, T. Kern, F., Müller, R., & Keller, A. (2022), BusyBee Web: towards comprehensive and differential composition-based metagenomic binning. Nucleic acids research, gkac298.
Laczny, C. C., Kiefer, C., Galata, V., Fehlmann, T., Backes, C., & Keller, A. (2017). BusyBee Web: metagenomic data analysis by bootstrapped supervised binning and annotation. Nucleic acids research, 45(W1), W171-W179.